EMAGE mouse gene expression spatial database

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EMAGE:17162

Fxr1 fragile X mental retardation gene 1, autosomal homolog ( MGI:104860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001545. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001545_01	euxassay_001545_02	euxassay_001545_03	euxassay_001545_04

euxassay_001545_05	euxassay_001545_06	euxassay_001545_07	euxassay_001545_08	euxassay_001545_09

euxassay_001545_10	euxassay_001545_11	euxassay_001545_12	euxassay_001545_13	euxassay_001545_14

euxassay_001545_15	euxassay_001545_16	euxassay_001545_17	euxassay_001545_18	euxassay_001545_19

euxassay_001545_20	euxassay_001545_21	euxassay_001545_22	euxassay_001545_23	euxassay_001545_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17162_wholemount_strong.wlz

17162_wholemount_moderate.wlz

17162_wholemount_weak.wlz

17162_wholemount_possible.wlz

17162_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17162_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4750
Entity Detected:	Fxr1, fragile X mental retardation gene 1, autosomal homolog ( MGI:104860)
Sequence:	sense strand is shown >T4750 CCACGCGTCCGCAACATGGCGGAGCTGACGGTGGAGGTTCGCGGCTCCAACGGGGCTTTCTACAAGGGAT TTATCAAAGATGTCCACGAAGACTCCCTCACAGTTGTTTTTGAAAATAATTGGCAACCAGAACGCCAGGT TCCGTTTAATGAAGTGCGATTACCACCACCACCTGATATAAAAAAAGAAATTAGTGAAGGAGATGAAGTA GAGGTATATTCAAGAGCAAATGACCAAGAGCCATGTGGATGGTGGCTGGCTAAAGTTCGGATGATGAAAG GCGAGTTTTATGTCATTGAATATGCTGCTTGTGATGCCACTTACAATGAAATAGTCACATTTGAACGACT TCGGCCTGTCAATCAAAATAAAACTGTCAAAAAAAATACCTTCTTTAAGTGCACAGTGGATGTTCCTGAG CACCTGAGAGAAGCGTGTGCTAATGAAAATGCCCATAAAGATTTTAAGAAAGCAGTAGGAGCATGCAGAA TCTTTTATCATCCTGAAACTACCCAGCTAATGATACTGTCGGCCAGTGAAGCAACTGTGAAGAGAGTAAA TATCTTAAGTGATATGCATTTGAGAAGTATTCGGACGAAGTTGATGCTTATGTCCAGAAATGAAGAGGCC ACTAAGCATTTAGAATGCACAAAACAACTTGCAGCAGCTTTTCATGAAGAATTTGTTGTGAGAGAAGATT TAATGGGCCTGGCGATAGGAACGCATGGCAGTAACATACAGCAAGCTAGGAAGGTTCCTGGAGTTACTGC CATTGAGTTAGATGAAGACACCGGAACGTTTAGAATCTATGGAGAGAGTGCTGAGGCTGTAAAAAAAGCT AGAGGTTTCTTGGAATTTGTGGAAGATTTTATTCAAGTTCCCAGGAATCTTGTTGGAAAAGTAATTGGAA AAAATGGCAAAGTTATTCAAGAAATAGTGGATAAATCTGGTGTGGTTCGAGTAAGAATTGAAGGAGACAA TGAAAATAAACTACCTAGAGAAGACGGAATGGTTCCATTTGTATTTGTTGGCACTAAAGAAAGCATTGGG AATGTGCAAGTTCTTCTAGAGTATCACATCGCTTACTTAAAGGAAGTGGAACAACTAAGAATGGAACGTC TGCAGATTGATGAGCAGCTGCGACAGATTGGTTCTAGGTCTTATAGTGGAAGAGGCAGAGGTCGTCGGGG CCCTAATTACACCTCCGGTTATGGTACAAATTCTGAGCTGTCTAACCCCTCCGAAACAGAATCTGAGCGT AAAGATGAGCTGAGTGATTGGTCATTGGCAGGAGAAGATGATCGAGAGACTCGACATCAGCGAGACAGCA GGAGACGCCCAGGAGGAAGAGGCAGAAGTGTTTCTGGGGGACGAGGTCGTGGTGGACCACGTGGTGGCAA ATCCTCCATCAGTTCTGTGCTCAAAGATCCAGACAGCAATCCATACAGCTTACTTGATAATACAGAATCC GATCAGACTGCAGACACTGACGCCAGCGAATCTCACCACAGTACTAACCGTCGTAGGCGGTCTCGTAGAC GGAGGACTGATGAAGATGCTGTTCTGATGGATGGACTGACTGAATCTGATACAGCCTCAGTTAATGAGAA TGGGCTAGGCAAAAGATGTGATTGAAGAGCATGGTCCTTCAGAAAAGGCAATAAATGGTCCAACTAGCGC TTCTGGCGATGAAATTCCTAAGCTACCGCGTACTCTGGGAGAAGAAAAGACTAAGACCTTAAAAGAAGAC AGCACTCAAGAAGCAGCAGTCCTGAATGGTGTTTCATAACTGAAGGAAGTTCCTAGTTTACAGTTCTTTT ACATTACAGTTACAATAGTGCTTGTACAAGCTTGCCAAAGATAGAATACGGATCGCCAGTCTTACATCGC ACTTTCAGTTCCTCCATTTGGAATTCAGAAAGGGGAGGGATCCTGAAGAAATCATATGTTAAACATACCT TGACACCTACTGTGTTAAAAATATATCATCAGATGTGCCTTGAGAATAGTATATGTAACATTAAAAAAAG TTGCTGGCTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5041635 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5041635 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17160 same embryo
	EMAGE:17163 same embryo
	EMAGE:17161 same embryo
	EMAGE:17159 same embryo
	EurExpress:euxassay_001545 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source