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EMAGE:17167

Phlda2 pleckstrin homology-like domain, family A, member 2 ( MGI:1202307)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17167 EMAGE:17167 EMAGE:17167 EMAGE:17167 EMAGE:17167
euxassay_001560_01 euxassay_001560_02 euxassay_001560_03 euxassay_001560_04 euxassay_001560_05
EMAGE:17167 EMAGE:17167 EMAGE:17167 EMAGE:17167 EMAGE:17167
euxassay_001560_06 euxassay_001560_07 euxassay_001560_08 euxassay_001560_09 euxassay_001560_10
EMAGE:17167 EMAGE:17167 EMAGE:17167 EMAGE:17167 EMAGE:17167
euxassay_001560_11 euxassay_001560_12 euxassay_001560_13 euxassay_001560_14 euxassay_001560_15
EMAGE:17167
euxassay_001560_16

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17167Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17167_wholemount_strong.wlz
17167_wholemount_moderate.wlz
17167_wholemount_weak.wlz
17167_wholemount_possible.wlz
17167_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17167_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4758
Entity Detected:Phlda2, pleckstrin homology-like domain, family A, member 2 ( MGI:1202307)
Sequence:sense strand is shown

>T4758
CCACGCGTCCGGCAACCTTTCGAAATGGCTTCGAAAATCGTGATGTCTTCGAAAACCGTGAAGACCTCCG
ACGAGATCCTTTGCGAGGGCGAGCTGGAGAAGCGAAGCGACAGCCTGTTCCAGGTATGGAAGAAGAAGCG
CTGCGTGCTCACCGCCGACCGCCTGCGCCTCTTCTCCGGGAAAACCAGCCCCGCCAAGGAGCTGTTTTTC
CACTCCATCCTCAAGGTGGACTGCGTGGAGCACACCTCTAAGTACGTGTACTTCACCATCGTCACCAACT
ATTACAAGGAGATCGACTTCCGCTGCACGGTGGAGAGCTGCTGGAACGCGGCCATCACCATGGCGCTGAT
CGACTTCCAGAACCGTCGGGCGCTGCAAGACTTTCCCCGCTACCGGTATCAGCGCTCTGAGTCTGAAATG
CCTTCCGAGCCGGGAGAGCAGAGCGCTCTGGGTCCGTGAAACGCACGGGACCAGTTGAGCCGGGAACCAG
CCCACGCTGCCGCATCAGACAGGAGCCCAGGAGCCCAGGGGATGCCCCGGGTGACGCCGCCGCTGCTCTT
CACCGAAGATATTCCCGTGCTTGCTGAGCCTTGCCCCGCTGTGCGGGTGCGCTAACTTATTGGACCGTAT
TTATATTGGTTACCTGCTTCCAACCCACCATTCCGTGTTAATATTTTTTATACCATATTTTCATTCCAAA
TAAACAATGTCACTTTTCTTTTTTTAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5042041 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5042041 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17165 same embryo
 EMAGE:17164 same embryo
 EMAGE:17168 same embryo
 EMAGE:17166 same embryo
 EurExpress:euxassay_001560 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS