EMAGE mouse gene expression spatial database

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EMAGE:17170

Nrd1 nardilysin, N-arginine dibasic convertase, NRD convertase 1 ( MGI:1201386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001559. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001559_01	euxassay_001559_02	euxassay_001559_03	euxassay_001559_04

euxassay_001559_05	euxassay_001559_06	euxassay_001559_07	euxassay_001559_08	euxassay_001559_09

euxassay_001559_10	euxassay_001559_11	euxassay_001559_12	euxassay_001559_13	euxassay_001559_14

euxassay_001559_15	euxassay_001559_16	euxassay_001559_17	euxassay_001559_18	euxassay_001559_19

euxassay_001559_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17170_wholemount_strong.wlz

17170_wholemount_moderate.wlz

17170_wholemount_weak.wlz

17170_wholemount_possible.wlz

17170_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17170_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4522
Entity Detected:	Nrd1, nardilysin, N-arginine dibasic convertase, NRD convertase 1 ( MGI:1201386)
Sequence:	sense strand is shown >T4522 GGCCTGTCCTCCCCAGCGGCTGGGGCAGCAACAACGCCGGCCGCCGCCGTCGCCCCTGGGACGCGGAGGA GGAGGAGTGGCTGGAGGTGTAGGAGGAGGAGGTGGGCCTGGGGAGCGGGATGTCCAGCGCTACGACTTGC TGGTGAATGCTGAGGAGAGTCGCGGTTGCTGCAGTCTGTGTCACCGGGAGGAAGTTGCGGTGTGAGGCCG GGCGGGAGCTCACGGCTCTGGGGCGAATCGAAGCACGGGGTCTGTGCGAAGAATCATCGAAACCCTTTCC TACGCTGACCATGCCTGGAAGAAACAAGGCGAAGTCCACCTGCAGTTGCCCGGACCTGCAGCCCAATGGA CAGGACTTGGGCGAGAGCGGCCGGCTTGCTCGTCTAGGAGCGGATGAATCTGAGGAGGAGGGACGGTCTT TCAGTAATGTCGGGGACCCTGAGATCATCAAGTCTCCCAGCGATCCCAAGCAGTACCGATACATCAAATT ACAGAATGGCTTGCAGGCACTTTTGATTTCAGATCTAAGTAATGTGGAAGGTAAGACAGGAAATGCAACA GATGAAGAGGAGGAAGAAGAGGAGGAGGAAGAGGAAGAAGATGATGATGATGACGACGACGACGACGATG ATGATGAAGACTCTGGAGCTGAGATACAAGATGATGATGAGGAAGGTTTTGATGATGAAGAGGAATTTGA TGATGACGATGATGATGAACATGATGATGATGATCTTGAGAATGAGGAAAACGAACTGGAAGAGTTGGAA GAGAGGGTAGAAGCCAGGAAGAAAACTACTGAGAAACAGTCTGCAGCGGCTCTGTGTGTTGGAGTTGGGA GCTTTGCTGATCCAGATGACCTGCCTGGGCTGGCACACTTTTTGGAGCACATGGTATTCATGGGTAGTTT GAAATACCCTGATGAGAATGGATTTGATGCCTTCCTCAAGAAACATGGAGGTAGTGATAATGCCTCAACT GATTGTGAACGCACAGTCTTTCAGTTTGATGTCCAGAGAAAGTATTTCAAGGAAGCCCTGGATAGATGGG CCCAGTTCTTTATCCATCCACTGATGATCAGAGATGCAATTGACCGAGAGGTGGAAGCTGTGGACAGTGA GTATCAGCTTGCAAGGCCTTCTGATGCAAACAGAAAAGAAATGTTGTTTGGAAGCCTCGCTAGACCGGGA CATCCTATGGGGAAGTTTTTTTGGGGAAATGCTGAGACACTCAAGCATGAACCAAAGAAGAATAACATTG ATACACATGCCAGACTGAGAGAATTCTGGATGCGCTACTACTCTGCTCATTACATGACCTTAGTTGTTCA GTCCAAAGAAACCCTGGACACTTTGGAAAAGTGGGTGACGGAGATCTTCTCTCAGATACCAAACAATGGG CTACCTAAACCAAACTTTAGCCATTTAACGGATCCTTTTGACACACCAGCATTTAACAAACTTTATAGAG TTGTTCCAATCAGAAAAATCCATGCTCTGACCATCACATGGGCACTTCCTCCTCAGCAGCAGCACTACAG GGTGAAGCCACTTCATTATATTTCCTGGCTTGTTGGACATGAAGGCAAAGGCAGCATTCTTTCTTATCTT AGAAAAAAGTGCTGGGCTCTTGCATTGTTTGGTGGAAATGGTGAGACAGGTTTTGAGCAAAATTCTACTT ACTCAGTGTTCAGCATTTCTATTACGCTGACTGATGAGGGCTATGAGCATTTTTATGAGGTTGCCCACAC TGTCTTTCAGTATTTAAAAATGCTGCAGAAGCTAGGTCCAGAGAAAAGAGTTTTTGAAGAGATTCAGAAA ATTGAGGATAATGAATTTCATTATCAAGAACAGACAGATCCAGTCGAGTATGTGGAGAACATGTGTGAGA ACATGCAGCTGTACCCACGTCAGGACTTTCTCACCGGAGATCAGCTTCTGTTTGAGTACAAGCCAGAAGT CATTGCTGAGGCCCTTAATCAGCTAGTTCCTCAGAAGGCAAATCTCGTTCTTCTCTCTGGTGCTAATGAG GGAAGATGTGACCTCAAGGAGAAGTGGTTTGGGACTCAGTATAGCATAGAAGATATTGAAAACTCTTGGA CTGAACTATGGAAGAGTAATTTTGACTTAAATCCAGACCTTCATCTTCCAGCTGAAAATAAGTACATAGC CACGGACTTTACGTTGAAGGCTTTTGATTGCCCTGAAACTGAATACCCTGCTAAAATTGTCAATACCGCA CAAGGCTGCTTGTGGTATAAGAAAGACAACAAATTCAAAATCCCCAAAGCATATATACGATTTCATCTGA TCTCACCTTTGATACAGAAATCTGCAGCAAATGTTGTCCTCTTTGACATCTTCGTGAATATTCTTACGCA CAACCTTGCCGAACCCGCCTATGAAGCTGATGTGGCACAGCTGGAGTATAAACTGGTAGCTGGAGAACAT GGGTTAATTATTCGAGTGAAAGGATTCAACCACAAACTACCTCTGCTCTTTCAGCTCATTATTGACTACC TGACTGAGTTCAGTTCCACACCCGCTGTCTTTACCATGATAACTGAGCAACTGAAGAAGACCTACTTCAA CATCCTCATCAAGCCCGAGACTCTGGCCAAAGATGTGCGGCTTTTAATTCTGGAATATTCCCGCTGGTCT ATGATTGACAAGTACCAAGCTTTGATGGATGGCCTTTCCCTTGATTCTCTGCTAAACTTTGTCAAGGATT TTAAATCCCAACTTTTTGTTGAGGGCCTGGTCCAAGGGAATGTCACCAGCACGGAATCGATGGATTTCCT AAAATATGTTGTTGACAAGCTGAACTTCGCGCCCCTGGAGCGGGAGATGCCTGTGCAGTTCCAGGTGGTG GAGCTGCCGAGCGGCCACCACCTGTGCAAAGTCAGAGCTCTGAATAAAGGGGATGCCAACTCTGAAGTCA CTGTGTACTACCAGTCAGGTACGAGGAGTCTGAGAGAATATACTCTTATGGAGCTGCTTGTGATGCACAT GGAAGAGCCCTGCTTTGACTTCCTTCGCACCAAGCAGACCCTTGGGTACCATGTCTACCCAACCTGTAGG AACACATCTGGAATTCTAGGATTTTCTGTAACTGTTGGGACTCAGGCAACCAAATACAACTCCGAAACTG TTGACAAGAAGATAGAGGAATTTCTTTCCAGCTTTGAGGAGAAGATTGAGAACCTCACAGAAGATGCATT CAATACTCAGGTCACAGCACTGATCAAGTTGAAAGAGTGTGAGGACACCCACCTTGGGGAGGAGGTGGAC AGGAACTGGAATGAAGTGGTGACACAGCAGTATCTCTTTGACCGCCTTGCCCATGAGATTGAAGCCCTGA AGTCTTTCTCAAAGTCAGACCTGGTCAGCTGGTTCAAGGCTCACAGAGGACCTGGAAGTAAAATGCTCAG CGTTCATGTTGTTGGATATGGGAAGTATGAACTGGAAGAGGATGGTGCCCCTTTTGGTGAGGATTCAAAT TCTCGTGAAGGGATGCAGCTAACCTACCTGCCACCCTCTCCTGTCCTGGCAGAATCCACCACCCCCATTA CTGACATCAGGGCCTTCACAGCAACACTCAGCCTCTTCCCCTACCATAAAATAGTCAAATAAACTGTAGT CTCATTGGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4971791 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4971791 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17171 same embryo
	EMAGE:17169 same embryo
	EMAGE:17172 same embryo
	EMAGE:17173 same embryo
	EurExpress:euxassay_001559 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source