EMAGE mouse gene expression spatial database

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EMAGE:17175

Ttc12 tetratricopeptide repeat domain 12 ( MGI:2444588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001586. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001586_01	euxassay_001586_02	euxassay_001586_03	euxassay_001586_04

euxassay_001586_05	euxassay_001586_06	euxassay_001586_07	euxassay_001586_08	euxassay_001586_09

euxassay_001586_10	euxassay_001586_11	euxassay_001586_12	euxassay_001586_13	euxassay_001586_14

euxassay_001586_15	euxassay_001586_16	euxassay_001586_17	euxassay_001586_18	euxassay_001586_19

euxassay_001586_20	euxassay_001586_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17175_wholemount_strong.wlz

17175_wholemount_moderate.wlz

17175_wholemount_weak.wlz

17175_wholemount_possible.wlz

17175_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17175_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18 19

4th ventricle lateral recess

strong

regional

strong expression: see section 03 04 05 06 07 09 10 12 13 14 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 11 12 14 15

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4535
Entity Detected:	Ttc12, tetratricopeptide repeat domain 12 ( MGI:2444588)
Sequence:	sense strand is shown >T4535 CACTAAAGAGATTTGTGTCTGCGATGGCTGATGATCAAGAGAGAGACTTGCAGAGATTTCTGGAAAATGT GGATGAAATAACCAGTTTAATCCAAGAGATGAATTCTGATGATCCCTTCATACAGCAGAAAGCTGTCCTG GACTCGGAAAAGAAACTCCTGCTGATGGAGAGAGAGCAAGAGGAGGACGGGTGCAGGACCACTCTGAATA AGACCATGATCAGTCCTCCACAAACTCCCGAGAATGCAGATGAAATGAGCCCAGATGCCTTCTTGGCATC CGTGGAGAAGGACGCCAAGGAACGAGCCAAGAGAAGACGAGAAAACAGAGTCCTGGCAGATGCCCTGAAG GAGAAGGGGAACGAGGCCTTTGTCAGGGGCGACTATGAAACAGCCATATTCTTCTACAGTGAGGGCTTGG GGAAGCTCAAGGACATGAAGGTGCTGTACACCAACCGAGCCCAGGCTTTTATAAAACTTGGGGACTATCA GAAGGCTCTTGTGGATTGTGACTGGGCTCTGAAGTGTGATGAGAATTGTACAAAGGCCTATTTCCACATG GGGAAAGCCCACGTGGCCCTGAAGAACTACAGCAAGGCTAAAGAATGTTATCAGAAGATTGAAGAGATAA ACCCCAAGCTGAAGGCACAGGTGAAAGAACACCTAAATCAAGTAACTCTTCGAGAGAAAGCAGATCTTCA AGAGAAGGAAGCCCAAGAATCTCTGGATTCAGGAAAGAACACAGCAGTGACGACCAAAAATCTCCTGGAA ACCCTATCCAAGCCTGGCCAGACACCCTTGTTCTATGCAGGAGGCATTGAGATTCTGACAGAAATGATGG CTGACTGCACAGAGCGAACGTTGTTCAGAACCTACGGTGGATTCAGCACCATCAGTGACCATGAGGTCAT CAGAAGGTGCCTCTTCTTAACAGGGAAGGATGCAGTCGAGGAGGTGCTTTGCGTATCTGTTCTAAAACTC TGGCAGGAGGTTTGCGTCGACAACGAGGAGAACCAGCGCCTGCTTGTGACCCACCCTGATATGGCCAGGC TGCTGCCTTCCTTCATGACTTCCAGAATCCTGGTGATTCAACAGCAGAGCCTGGACCTACTGCTGCAGCT CACTCAGACGGAGAATGGCCGGAACCAAGTGATCAAGCACCTTGACATGACCCAACTGTGTGAAGCACTG CTGTCATTTCTTGCCTTCTCTGATAAGAAGGCCAATACAGCCATCGGAATACTCACAGACTTGGCTCTAG AAGAAAGATTCCAAGTCTGGTTCCAGACCAACCTTCCAGATGTCCTCCCTGCTCTCACGGGAGCTCTGAA TCGAGAGCCCAAGATAACCAGCCCCTCTGCCCTCTGCCAGTGCATTGCTCTGTTGGGAAACATCAGCGCT GAGCCAACTGCCAGAAAACACATGATAGCTCATGAAGAATTTGGGAATGCCTGCTTGGACCTCCTGGTCA AGTGTGAAGAGGACATGGACTTATTCAGAGAGATCACATATACCCTCCTAGGACTCATCATGAACTTGTG TCTTGAAATCACCTTCCTCTCTGAGATGTGGGTTGTGAAGATGAGCCGGAAGTGCCTGTCTCTACTGAAC AGCAAGGATGGAGGAATCTTAACAAGAGCGGCAGGTGTTCTGAGCCGGACCCTTTCTTCCTCTCAGACCA TTGTGGAGGAGGCCCTGACAGCAGGAGTGGTCAAGAAAATGATTAAATTCCTGAGGATGGGAGGCCAGAC TGCATCACGTTACGCCATAAAGATACTGGCTATCTGCACGAACAGCTGTCATGCAGCTCGGGAAGAAGTA GTGAGACTTGATAAAAAGTTTGACCTTTTGATGCTGCTGCTTGCCTCTGAGGATGAAATTCTGGCGGGCA ATGCTGCCCTCTGCCTTGGCAACTGCATGGAAGTGCCCAGTGCAGCCCCTTCCTTGCTGAAGACGGACAT CGTGAGGGTGCTCTTGAAGCTGGCAGGCAGCGACTCAAAGGAGACAGGCGTACAGCTGAATGCAGGCATC GCTCTGGGGAAGCTGTGCACTGCTGAGCCCAGGTTTGCTGCCCAACTGCGAGAGCTTCACGGGATACAAG TTCTCAACTCTACAATGAAACACTTGAATGATTCTTGAAAGACACGGTGCCAGTGTAAAAGCCTGTGAGC ACACACACACACACACACACACACAACTCTGGGCCAGGGGTCACTGCTCTGCTAATAAATTCTTTTTAAA TATTTAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4974294 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4974294 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17177 same embryo
	EMAGE:17174 same embryo
	EMAGE:17176 same embryo
	EurExpress:euxassay_001586 same experiment
	MGI:4828977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source