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EMAGE:17195

Car5a carbonic anhydrase 5a, mitochondrial ( MGI:101946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195
euxassay_001592_01 euxassay_001592_02 euxassay_001592_03 euxassay_001592_04 euxassay_001592_05
EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195
euxassay_001592_06 euxassay_001592_07 euxassay_001592_08 euxassay_001592_09 euxassay_001592_10
EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195
euxassay_001592_11 euxassay_001592_12 euxassay_001592_13 euxassay_001592_14 euxassay_001592_15
EMAGE:17195 EMAGE:17195 EMAGE:17195 EMAGE:17195
euxassay_001592_16 euxassay_001592_17 euxassay_001592_18 euxassay_001592_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17195Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17195_wholemount_strong.wlz
17195_wholemount_moderate.wlz
17195_wholemount_weak.wlz
17195_wholemount_possible.wlz
17195_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17195_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4792
Entity Detected:Car5a, carbonic anhydrase 5a, mitochondrial ( MGI:101946)
Sequence:sense strand is shown

>T4792
CCACGCGTCCGCCCACGCGTCCGACCTAACTCTGAGGTGGCCCTGACCAAAGCAAGGGCATACGGTGGGC
CACCACCCCGCACTGCTCAGAGCCAAGATGCTCAGGAGAGACCCCCGCAAGCCCTTAGCCATCCTCAGGC
ATGTGGGACTTCTCTGTGCCACAGGGCCACAGCGCTGGCGTTTCCAGCATTCCTGTGCAGAGGAACACAG
CAACTGTGCCCGACACCCCCTCTGGACTGGCCCAGTGTCCTCAGCAGAAGGCACCCGGCAGTCTCCCATC
AACATCCAGTGGAAGGACAGTGTGTATGACCCGCAGCTGGCACCACTCAGGGTCTCCTATGATGCCGCGT
CCTGCAGATACCTCTGGAACACTGGCTACTTCTTCCAGGTGGAGTTTGACGATTCCTGTGAGGATTCAGG
GATCAGCGGTGGGCCTCTGGGAAACCACTACAGGCTGAAGCAGTTTCATTTCCACTGGGGAGCAACAGAT
GAATGGGGCTCAGAGCACGCAGTGGACGGCCATACCTACCCAGCTGAGCTCCATCTGGTTCACTGGAATT
CCACGAAATATGAAAATTACAAGAAAGCCTCCGTGGGGGAGAACGGGCTGGCGGTCATCGGCGTGTTTCT
GAAGCTCGGGGCGCATCACCAGGCCCTGCAGAAGCTGGTGGATGTCTTGCCGGAAGTAAGACACAAGGAT
ACACAGGTGGCCATGGGACCCTTTGACCCCTCTTGTCTGCTGCCTGCCTGCCGGGATTACTGGACCTACC
CTGGCTCCCTCACCACCCCGCCACTTGCTGAGTCAGTCACCTGGATTGTGCAGAAGACGCCTGTTGAGGT
GTCCCCGAGCCAGCTATCCACGTTCCGCACACTCTTGTTCTCCGGGCGAGGTGAGGAAGAGGATGTGATG
GTGAACAACTACCGCCCGCTCCAGCCCCTCAGGGACCGCAAACTTCGCTCGTCCTTCCGGCTTGATCGGA
CCAAGATGAGGTCTTAATCTCAGGATGAAGTCTGTAAGGATAGGCAGAGCAGACCGGAAAGGGGGCCCGA
GTTTCCAGGGTGCCACGTGGCACGACGAGCCGGGGCGGCAGTGGCGCACGCCTTTAATCCCAGCACATGG
GAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAG
GACTACACAGAGAAACCCTGTCCCGGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5051543 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5051543 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17192 same embryo
 EMAGE:17191 same embryo
 EMAGE:17194 same embryo
 EMAGE:17193 same embryo
 EurExpress:euxassay_001592 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS