EMAGE mouse gene expression spatial database

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EMAGE:17204

Acad8 acyl-Coenzyme A dehydrogenase family, member 8 ( MGI:1914198)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001604_01	euxassay_001604_02	euxassay_001604_03	euxassay_001604_04

euxassay_001604_05	euxassay_001604_06	euxassay_001604_07	euxassay_001604_08	euxassay_001604_09

euxassay_001604_10	euxassay_001604_11	euxassay_001604_12	euxassay_001604_13	euxassay_001604_14

euxassay_001604_15	euxassay_001604_16	euxassay_001604_17	euxassay_001604_18	euxassay_001604_19

euxassay_001604_20	euxassay_001604_21	euxassay_001604_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17204_wholemount_strong.wlz

17204_wholemount_moderate.wlz

17204_wholemount_weak.wlz

17204_wholemount_possible.wlz

17204_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17204_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 07 08 11 15 17 18 19 20

4th ventricle lateral recess

strong

regional

strong expression: see section 03 04 05 06 07 08 09 11 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4805
Entity Detected:	Acad8, acyl-Coenzyme A dehydrogenase family, member 8 ( MGI:1914198)
Sequence:	sense strand is shown >T4805 CCACGCGTCCGCTGAGATTCGAAGTTTACATGGCGATGTTGCGGTCCGGCTACCGGCGCTTTGGCTGTCT GCGCGCGGCACTCAAGTCCCTGGCTCAGACCCACCACCGGAGCATCACCTTCTGCATCGATCCTTCCTTG GGGCTAAATGAAGAACAGAAAGGATTTCAGAAAGTGGCCTTTGACTTTGCAGCTCGGGAAATGGCTCCCA ATATGGCGGAGTGGGATCAGAAGGAGCTGTTCCCTGTGGATGTGATGCGGAAGGCTGCACAGCTGGGATT TGGGGGGGTCTATGTGCGAACAGATGTGGGTGGATCTGGACTGTCTCGCCTTGATACTTCTGTCATCTTT GAAGCCTTGGCCACAGGCTGCACCAGCACCACTGCCTATATAAGCATCCACAACATGTGTGCCTGGATGA TTGATAGCTTTGGAAATGAGGAACAGAGGCACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGC TTCCTACTGCCTCACTGAACCAGGCAGTGGGAGTGATGCTGCATCTCTTCTGACCTCAGCTAAACAACAA GGAGATCATTACATCCTCAATGGTTCCAAGGCCTTCATCAGTGGGGGAGGTGAGTCAGACATCTATGTGG TCATGTGCCGAACTGGTGGGTCTGGCGCCAAAGGTATCTCCTGCATAGTTGTTGAGAAAGGAACCCCTGG TCTCAGCTTTGGCAAGAAGGAGAAGAAGGTGGGGTGGAACTCGCAGCCAACCCGAGCAGTGATCTTTGAA GACTGTGCTGTCCCTGTGGCCAACAGGATTGGGACCGAGGGGCAGGGTTTTCTCATCGCCATGAAAGGAC TGAATGGTGGAAGGATCAATGTTGCATCCTGCTCTCTGGGAGCCGCTCATGCTTCAGTTATCCTAACCCA AGAACACCTCAAGGTCCGCAAGCAGTTTGGAGCACCTTTGGCCAGAAGCCAGTACCTCCAATTCCAGTTG GCAGATATGGCCACCAAGCTAGTTGCTTCCCGGCTGATGATCCGTACTGCAGCAGTGGCTTTGCAGGAAG AGCGGGAAGATGCAGTGGCCCTGTGTTCCATGGCCAAGCTCTTTGCTACAGAGGAGTGCTTTGCTATCTG CAACCAGGCTTTGCAGATGCATGGGGGCTACGGCTATCTGAAGGACTATGCTGTTCAACAGTATATGCGG GACTCCAGGGTCCACCAGATCCTAGAAGGTAGCAATGAAGTGATGAGGATGCTGATCTCTCGAAACCTGC TTCAGGACTAGCTCCCAAGCTGGAGAGCCTCGCCTGGAGTCTGTGTGCAGCTGGAATCAGTCTTGATGCT CCTATGGGCTGCTCAGTTCAAGTGAATCATGAAGTAGACCAAAGGGCTGAGCTCTTCTAAACAGACTGAG CTGTCAGCCACGCTGCTGGTTCTGAGACAAGAACAGAGTTCTTAGCAGAGCTAGAAGGGCTGCTCTGAGG AGCCATGCCCAAAGAAGACCTGCACACTGCCTTCCTCATTCTGGAAAGACAAGCGGTCAAAGCTGTGGCT TGGATCCACACTATGGGCATTTTGGTGATTCTTGTCCTTAGGGTCTGGGTACTTGAATGAAGCTTTTCCT GATTAAACAGGTAAAGTCTGGGGAGGCCAGGGCAGTGGACAGATCTGCCCCTTTCTGGTTTCTGTTCTGC ACTAGGGGTATGTCAGGACAGTACTCCTGTACTCAGATCATGATGAAATAAATATTTGGGAACCTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5053565 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5053565 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17206 same embryo
	EMAGE:17205 same embryo
	EurExpress:euxassay_001604 same experiment
	MGI:4822897 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source