EMAGE mouse gene expression spatial database

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EMAGE:17219

Zscan22 zinc finger and SCAN domain containing 22 ( MGI:2443312)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008870. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008870_01	euxassay_008870_02	euxassay_008870_03	euxassay_008870_04

euxassay_008870_05	euxassay_008870_06	euxassay_008870_07	euxassay_008870_08	euxassay_008870_09

euxassay_008870_10	euxassay_008870_11	euxassay_008870_12	euxassay_008870_13	euxassay_008870_14

euxassay_008870_15	euxassay_008870_16	euxassay_008870_17	euxassay_008870_18	euxassay_008870_19

euxassay_008870_20	euxassay_008870_21	euxassay_008870_22	euxassay_008870_23	euxassay_008870_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17219_wholemount_strong.wlz

17219_wholemount_moderate.wlz

17219_wholemount_weak.wlz

17219_wholemount_possible.wlz

17219_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17219_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4803
Entity Detected:	Zscan22, zinc finger and SCAN domain containing 22 ( MGI:2443312)
Sequence:	sense strand is shown >T4803 CCCACGCGTCCGCCCACGCGTCCGCTGGAGACCCGGTGAGCGCGGGAACCCTGGCATGGAGGGGTGAGGG CTGCGAACGCTCACGGCAAACCTAACGGCCTGATGGGAGCCAGGAGTGGAACGTGAAGAGGCAAGTTGCA AACAGAGCAATACAGATGAATTGGAGCCACCAAAAATGGCGACTGAAACTGTCATGGGAAGCGTTTTGCC AAAATCTACCCTTGCCCACACTTGTAAACCTGAGAGCCACTCAGAGAGCCAGCCAGAACTCCTAGGAGCA CTCTGGATGAAGTCTACTGCCCAAGAGATGGATTTCAGAAAAGCTCTGGGGCCTCACATGGATGCCCCCA AAGACCAGCCTGGCCATGAGTCTAACACCTCAGGAAACGGTTCCAACATGTGGCCCAACTTCCCTTCCCA AGACAAGGCTTCTTCAGAGGAGAAATTTGGCCCATTACTTGATAATGAGACAGTGCCTCCAGATACTTGC TCAGAGAAGAAATCCTCCAAGGATAGTGAATGTTTGAAAACATTCCAGAACACTTCAGCTCTGGAGGCCC ACCAGAAGAGCCACTCTCAGAAGACGCCCTATGCCTGTACTGAGTGTGGGAAGGTATTCAGTCGGAGCAC CCACCTTGTCCAACACCAGGTTGTTCACACAGGAGCAAAGCCCCATGCGTGTAAAGAGTGTGGTAAAGCC TTCAGTCGCGTTGCCCACCTGACACAGCACCTGAGGATTCATACTGGGGAGAAGCCTTATAAATGTGAGG AATGTGGTAAAACCTTCAGCCGCAGCACCCACCTCACGCAGCACCAGCGAGTACACACAGGCGAGCGGCC CTACGAGTGTGATACTTGCGGCAAAGCCTTCAGCCAGAGCACCCACCTGACACAGCACCAGCGCATCCAC ACCGGGGAGAAGCCCTACAGGTGTGATGTGTGTGGGAAAGCCTTCAGTGACTGTTCAGCTCTGGTCCGGC ACCTGAGAGTCCACTCTGGAGAGAAGCCTTATCAATGTAAGGACTGCCCGAAGGCCTTCGCACAAAGCTC CTCCCTCATTGAGCACCAGCGCACTCACACAGGTGAAAAGCCTTATAAATGCAGTGACTGTGGGAAAGCT TTTAGCCGCAGCTCAGCTCTCATGGTGCACCTAAAGATCCATATCACCGTGACTCGAAGCACCCCTTAGA GTACAGCACAGCCTCCCTGAGAGGATTGACATCTAAGAAATCTGATAATTGTGCTACCCAGTGTCTAAGG GGTTATCTGGGAACACCTCTACATTTGAGGTCCCAGAGGAAATCCCCTAATGAACACGGATTCAGACCAA TTGGTGAAGCTTCTAGAAGCTTCCTACTGCCCTCTGTATCCCACTCAGGCTGGTCCCAGAGAGAAGATAC TAAACTGATGATCTGCATTATTTCTTGGAACCAGAGCAAGTAAAGCCAGGTGTCTTTCCAGGCTCAGATG CCCCAAGATCCTCGAGGCAGGAAGACCCCAAGCTTCAGGTTCTCACATAGTTACTGTTCTTGCCGGTTCC TTGCTGGTCAGCCTAGAATGGCCCACTCTGTGCCTTCGTACCTGCTTCCCCACAATATTACTTTATGCCT AACTCCAAGACAGACTTGGGAGGCTGCCTCTCCATGCTATCCCTCTCATGTTCTCCCTGCTTTAATCCAC AGTGTCTGTCTTCTCCTCCCACGTCTTCCTTCCACATCTCAGGCTCCAGCCCTGTGGATTCACCGCAGGC TCATCTCTAATCTAGCCCCTCCTGCAGTATCACCTCCTGTGAGCTCACCTTGGGCCTCCCACTGGATTTT TATCAGGGCTCTCCTTTCTCACCTCTTCCTGCCTTCATTCCATCCCCACCCACTTTCTTCCTGGCGCATC TCTTTCTGCTACCCAGATCACAGTGAATCATTCCATGACACAATATGTTGTCAGTGAGTCACCTCTTCAA CAAACAAGCATGTCAGGATATATGTCATTTTGGTCCAAGCAATTACTCTAGGTATCCTGAAATGTTGTCA AAGGAATGTTGTCATCTGTCTTTGAGCACAGTGTGTGAAAGTGATTTCAAAGTACTGGTCTGCCTGTTGG GCAAACTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5053169 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5053169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17220 same embryo
	EurExpress:euxassay_008870 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source