EMAGE mouse gene expression spatial database

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EMAGE:17239

Dhx40 DEAH (Asp-Glu-Ala-His) box polypeptide 40 ( MGI:1914737)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001620. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001620_01	euxassay_001620_02	euxassay_001620_03	euxassay_001620_04

euxassay_001620_05	euxassay_001620_06	euxassay_001620_07	euxassay_001620_08	euxassay_001620_09

euxassay_001620_10	euxassay_001620_11	euxassay_001620_12	euxassay_001620_13	euxassay_001620_14

euxassay_001620_15	euxassay_001620_16	euxassay_001620_17	euxassay_001620_18	euxassay_001620_19

euxassay_001620_20	euxassay_001620_21	euxassay_001620_22	euxassay_001620_23	euxassay_001620_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17239_wholemount_strong.wlz

17239_wholemount_moderate.wlz

17239_wholemount_weak.wlz

17239_wholemount_possible.wlz

17239_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17239_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5650
Entity Detected:	Dhx40, DEAH (Asp-Glu-Ala-His) box polypeptide 40 ( MGI:1914737)
Sequence:	sense strand is shown >T5650 GGTGTTCGCTTCTTCTCCGGACCGGGTCTATGTCCCGGTTTCCCGCCGTCGCGGGCAGGGCGCCAAGGCG GCAGGAGGAAGGCGAGCGGCCAATAGAGCTCCAGGAAGAGCGGCCGTCAGCTGTTCGCATCGCTGACAGA GAAGAGAAAGGATGCACATCACAGGAGGGAGGAACAACTCCAACGTTTCCTATTCAGAAACAAAGAAAAA AGCTCATTCAAGCTGTGAGGGACAACTCGTTCCTCATCGTTACTGGAAATACAGGAAGTGGTAAAACAAC TCAACTCCCTAAATACCTTTATGAAGCAGGGTTTTCACAACATGGTATGATTGGTGTGACTCAGCCACGA AAAGTAGCCGCCATATCAGTTGCTCAGAGGGTTGCTGAGGAAATGAAATGCACTTTGGGATCTAAAGTAG GATACCAAGTTCGTTTTGATGATTGCAGCTCCAAGGAAACAGCAATCAAATACATGACTGATGGATGTTT ACTCAAACATATTTTGGGAGATCCAAATCTTAGCAAATTCAGTGTCATTATTTTGGATGAAGCCCATGAA AGAACTTTAACTACAGATATCTTATTTGGTTTACTGAAGAAGCTGTTTCAGGATAAGTCTCCTAACAGGA AAGAGCATTTAAAGGTGGTAGTGATGTCAGCAACTATGGAACTAGCCAAGCTCTCTGCATTCTTTGGAAA TTGCCCCATATTTGATATACCTGGAAGACTCTATCCAGTCAGAGAGAAATTCTGCAATTTGATCGGACCA CGAGACAGAGAAAATACTGCATATATTCAAGCGATTGTGAAAGTGACCATGGATATTCATTTGAATGAAA TGGCTGGAGACATCTTAGTTTTTCTGACTGGCCAGTTTGAGATAGAGAAGAGTTGCGAGCTGCTCTTTCA GATGGCGGAGTCTGTTGACTATGATTATGATGTACAAGATACCACGCTAGATGGCTTGTTGATTCTGCCA TGTTACGGCTCTATGACAACAGATCAACAGAGGAGAATATTTTTGCCACCTCCTCCTGGAATTAGAAAAT GTGTTATATCCACCAATATTTCTGCAACATCTTTGACAATAGATGGGATCAGATATGTGGTGGATGGAGG CTTTGTGAAGCAATTAAATCACAACCCAAGATTAGGGCTGGACATCCTGGAGGTGGTTCCTATTTCAAAA AGTGAAGCATTACAACGCAGTGGCCGTGCGGGCAGGACCGCCTCAGGAAAGTGCTTTAGGATTTACAGTA AAGAGTTTTGGAGCCAGTGTATGCCTGACCATGTGATCCCAGAGATTAAGAGAACTAGTTTGACATCTGT AGTTCTGACATTAAAGTGCCTTGCCATACATGATGTCATAAGGTTTCCATATTTGGATCCACCTAATGAG AGACTTATTTTGGAAGCACTTAAACAACTTTATCAGTGTGATGCTATTGACAGGAGTGGCCATGTGACAA GATTGGGATTATCCATGGTGGAGTTCCCTCTCCCCCCACATCTGACGTGTGCAGTGATCAGGGCTGCTTC TCTTGACTGTGAAGATTTGCTGCTTCCAATAGCAGCAATGCTATCTGTGGAAAATGTCTTCATCAGACCT GTTGATCCAGAGTACCAGAAGGAAGCAGAACAGAGACATCGGGAATTGGCAGCGAAAGCTGGAGGATTTA ATGACTTTGCAACTTTAGCTGTCATTTTTGAACAGTGCAAATCAAGTGGAGCTCCGGCTTCGTGGTGCCA GAAGCACTGGATTCACTGGCGGTGCTTATTCTCTGCATTTCGTGTGGAAGCTCAACTTCGGGAACTGATC AGGAAACTAAAGCAGCAAAGTGATTTCCCAAGAGAGACTTTTGAAGGCCCTAAACATGAAGTACTACGAA GATGTCTTTGTGCAGGCTACTTCAAAAATGTTGCTCGAAGATCTGTTGGCAGAACATTTTGCACAATGGA TGGGCGTGGAAGTCCAGTTCATATCCATCCTTCCTCAGCACTTCATGAACAGGAAACCAAACTTGAATGG ATAATTTTTCATGAAGTATTAGTTACCACCAAAGTCTATGCAAGAATTGTGTGCCCAATCCGTTATGAAT GGGTGAGAGACTTACTACCCAAGTTGCATGAACTTAATGCGCATGATTTGAGCAGTGTGGCTAGACGTGA AATGAGAGAAGATGCAAGAAGAAAATGGACAAATAAGGAAAATGTGAAACAGCTAAAGGATGGAATATCA AAAGAAGTTCTAAAGAAAATGCAAAGAAGAAATGATGACAAATCCATATCAGATGCACGGGCTCGCTTCC TTGAGAGAAAGCAACAGAGAATCCAGGACCACAGTGACACATTAAAGGAAACTGGCTAAGCAGTGGACTA TCCAATTCAGGAAGCAGGAAAGCAGCCAGGAAATGTGCTTCTGTTTTGCCAGTTATTTCCGACAGTACTA TCAGAAGGAAGTGGTCAGCAGCTGTTCCTAGCCTGTGAGCCGAAAGCAAATCAGAATTTATAAATCACAT CTCATCAATACTAACAAATGACATTTTGAAAGGAAAGATTGGGTTGCATAGTCATAGTACATGAATCCAA TCAAAGATGATACAATATTTCCCTCACTTCTGTTTTGCTGGCCTTAACTGGTATCAAACAGTGTCACTGA GATATTTTCAAAGAACACTGAATTGTATTTAATCAGCGTGTATTCCATTTGCATTGAAGCATTAAAGATT ATTTTCCTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6515479 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6515479 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17240 same embryo
	EMAGE:17242 same embryo
	EMAGE:17238 same embryo
	EMAGE:17241 same embryo
	EurExpress:euxassay_001620 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source