EMAGE mouse gene expression spatial database

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EMAGE:17241

Spint1 serine protease inhibitor, Kunitz type 1 ( MGI:1338033)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001628_01	euxassay_001628_02	euxassay_001628_03	euxassay_001628_04	euxassay_001628_05

euxassay_001628_06	euxassay_001628_07	euxassay_001628_08	euxassay_001628_09	euxassay_001628_10

euxassay_001628_11	euxassay_001628_12	euxassay_001628_13	euxassay_001628_14	euxassay_001628_15

euxassay_001628_16	euxassay_001628_17	euxassay_001628_18	euxassay_001628_19	euxassay_001628_20

euxassay_001628_21	euxassay_001628_22	euxassay_001628_23	euxassay_001628_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17241_wholemount_strong.wlz

17241_wholemount_moderate.wlz

17241_wholemount_weak.wlz

17241_wholemount_possible.wlz

17241_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17241_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5653
Entity Detected:	Spint1, serine protease inhibitor, Kunitz type 1 ( MGI:1338033)
Sequence:	sense strand is shown >T5653 CTGGGGCGGACGGTAGGCGGACGCCCAGCGGGCTCCGGGCATGTTTGGCGGACGTCCGCTCTGTGTTGGG GTCACAAGCACCCTCGGAACCCGGAGGCTCGCACTGTGAAGGTGACCCCCGCCTCGGGGAGGAGGGCGAC GGCCCCTGGGAGCACGATGGCGGGCCGCCGCCTGGCGCGGGCCAGCATCTCTGCGGTCGGCGTATGGCTC CTTTGCGCGCTTGGCCTCCAGGCCACCGAGGCCGAGCTGCCGTCCGCACCCGCTGAGCTTCCCGGGGGAG CCGCCTGCTTGAGCCGCTTTACCAGCGGGGTGCCGTCCTTTGTGCTGGACACCGAAGCCTCAGTCAGCAA CGGGGCCACCTTCCTGGGTTCCCCGACGGCGCGCCGCGGCTGGGACTGCGTGCGCTCCTGCTGCACCACT CAGAACTGCAACCTGGCGCTGGTGGAGCTGCAGCCAGACGGCGGCGAGGACGCCATCTCTGCCTGCTTCC TCATGAACTGCCTGTACGAGCAGAACTTCGTGTGCAAGTTCGCTCCGAAGGAGGGCTTCATCAACTATCT TACCCAGGAGCTTTACCGCTCCTACCGCGAGCTGCGGACTCGAGGCTTTGGAGGGTCCCGGATCCCTCGA ATCTGGATGGGCATAGACTTGAAGGTCCAGCTGCAGAAGCCCCTGGTACTGAACGAGGCAGACAACACAG ACTGGCACCTACTGCAAGGTGACAGTGATGTCAGAGTAGAGAGAAAACGTCCGGAAGAAGTGGAGCTGTG GGGACTTAAGGAGGGGACCTACCTGTTCCAACTGACAAGAACTGACTCAGACCAACCAGAGGAAACAGCC AACCTGACTATCACTGTGCTAACTGCCAAGCAGACAGAAGATTATTGCCTCGCATCCTACAAGGTGGGCC GCTGCCGGGGCTCCTTCCCACGCTGGTACTACGACCCCAAGGAACAGATCTGCAAAAGTTTTACTTTTGG AGGTTGCCTGGGCAACAAGAACAACTACCTTCGTGAGGAAGAGTGCATGCTAGCCTGCAAGGATGTGCAA GGAATCTCACCGAAAAGGCACCATCCAGTGTGCTCCGGCAGCTGCCATGCCACCCAGTTCCGCTGTAGCA ATGGCTGCTGTATCGATGGCTTCCTGGAGTGTGATGACACGCCAGACTGCCCTGATGGCTCCGACGAGGC CACCTGTGAAAAATACACCAGCGGCTTTGATGAGCTTCAGAATATCCATTTCCTCAGTGACAAAGGGTAC TGTGCAGAGCTGCCAGACACTGGGTTTTGCAAGGAGAACATCCCACGCTGGTATTACAACCCATTCAGTG AACGCTGTGCCCGATTCACCTATGGTGGTTGCTATGGGAACAAGAACAACTTTGAGGAGGAACAGCAGTG CCTTGAGTCCTGCCGTGGCATCTCCAAGAAGGATGTGTTTGGTCTTCGGAGGGAAGGCTCCATTCCCACT GTAGGCTCCGCTGAGGTAGCTATTGCTGTATTTCTGGTCATCTGCATCATAGTGGTCTTAACCATCCTGG GCTACTGTTTCTTCAAGAACCAAAGAAAGGAATTCCACAGTCCCCTGCACCACCCACCTCCCACACCAGC CAGCTCCACTGTTTCCACCACAGAGGACACAGAGCACCTGGTCTATAATCACACAACCCAGCCTCTCTGA GCCTGGGACTCTTGCCAGTCTTACCAGGTCCTGCTTGCCAAGACAGAAGCTAGAACCTGGAAAAACTTGG GGACCAGACTCTTCCTACCTCTTTCCTGGGCATACTTACTCTGTCTCAGAAGACAGATCTCTGGGCCTCT CGCAGGAGTCTCAGCTGCACTCAGGCCAGTTCTGGAGAAGGCCCAAGAGTCTGGAAGGAGCAGAGCAGCC TTGAGCCAGGAGTACTGTATATATGTCTTAGTCAGGGTTTCTATTCCTGCACAAACATCATGACCAAGAA ACAAGTTGGGGAGGAAAGGGTTTATTCGGCTTACACTTCCATACTGCTGTTCATCACCAAGGAAGCCAGG ACTGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGCCATGGAGGGATGTTCTTTACTGGATT GCCTCACCTGGCTTGCTCAGCCTGCTTTCTTATAGAACCAAGACTACCAGCCCTGAGATGGTCCCACCCA CAAGGGGCCTTTCCCCCTTGATCACTAATTGAGAAAATGCCTTACAGTTGGATCTCATGGAGGCATTTCC TCAACTGAAGCTCCTTTCTCTGTGATAACTCCAGCTGTGTCAAGTTGACACAAAACTAGCCAGTACAATT GACCCCTTGTCAACTTGACACACAAAAACATCACTAGTAAGCCTCAACCCTTACAATCTTATTCATCCCC ATTCTAAATAACTTTAAAAGTCCCACAGTCTTTACATATTCTTAAAATTTCAATCTCTTTAAAATATCCA AAGTCTTTTTACAATTAAAAGTAAACTGTGGGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6528808 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6528808 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17240 same embryo
	EMAGE:17239 same embryo
	EMAGE:17242 same embryo
	EMAGE:17238 same embryo
	EurExpress:euxassay_001628 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source