EMAGE mouse gene expression spatial database

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EMAGE:17265

Tcf3 transcription factor 3 ( MGI:98510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001651_01	euxassay_001651_02	euxassay_001651_03	euxassay_001651_04	euxassay_001651_05

euxassay_001651_06	euxassay_001651_07	euxassay_001651_08	euxassay_001651_09	euxassay_001651_10

euxassay_001651_11	euxassay_001651_12	euxassay_001651_13	euxassay_001651_14	euxassay_001651_15

euxassay_001651_16	euxassay_001651_17	euxassay_001651_18	euxassay_001651_19	euxassay_001651_20

euxassay_001651_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17265_wholemount_strong.wlz

17265_wholemount_moderate.wlz

17265_wholemount_weak.wlz

17265_wholemount_possible.wlz

17265_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17265_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5682
Entity Detected:	Tcf3, transcription factor 3 ( MGI:98510)
Sequence:	sense strand is shown >T5682 GCGCCGGCGGCTGCGGGCGTAGCGGGCCACCGCGGGCCACCGCCGCGCGCCGCCGCCTCTGCTACAGTCC CTTCCCGCGGGGCCTGCTCTGAGAGAAGCTCGAGAGAGACCAGGCGACGCGAACGCGAGTGGGGAGGAGG AAGGACGCGCGACCCCGAGCCCTGCGCGCTCCCGCCGCCCACGCGCGACCCTCGGGGACGCGCCCGCCAC CCTTTTGTCCCCGGGGTCCCCGAGGGCGGTGGGCAGCAGGGAGCCCCGGTGCACCCGGTGCATGCCCCCG CCCAGCAGGGCTGTCTCTAGACCTGGGGGACGCACCCCAGTTCCAACACCTGCTGTCCTGGGTGGATGAT GAACCAGTCTCAGAGAATGGCACCCGTGGGCTCTGACAAGGAACTGAGTGACCTCCTGGACTTCAGCATG ATGTTCCCGCTACCTGTGGCCAATGGGAAGAGCCGGCCCGCCTCCCTCGGGGGAACCCAGTTTGCAGGCT CAGGACTGGAGGACCGACCCAGCTCAGGCTCCTGGGGCAGCAGTGACCAGAACAGTTCTTCCTTTGACCC TAGCCGGACATACAGCGAAGGTGCCCACTTCAGTGACTCCCACAGCAGCCTGCCGCCTTCCACGTTCCTA GGAGCTGGGCTTGGAGGCAAGGGCAGTGAGCGGAATGCCTATGCCACCTTTGGGAGAGACACCAGTGTTG GCACCTTGAGTCAGGCTGGCTTCCTGCCAGGTGAGCTGAGCCTCAGCAGTCCCGGGCCACTGTCCCCATC GGGCATCAAGAGCAGCTCCCAGTATTACCCCTCATTCCCCAGCAACCCTCGTCGGAGAGCTGCAGATGGT GGCCTGGATACTCAGCCGAAGAAGGTCCGGAAGGTTCCGCCTGGTCTCCCTTCCTCGGTGTATCCGCCCA GCTCAGGTGACAGCTACAGCAGGGATGCTGCAGCCTACCCCTCCGCCAAGACCCCCAGCAGCGCTTACCC CTCCCCCTTCTACGTGGCAGATGGCAGCCTGCACCCATCAGCTGAGCTCTGGAGTACGCCTAGCCAGGTG GGCTTTGGGCCCATGCTAGGTGACGGCTCTTCCCCTCTGCCCCTTGCACCGGGCAGCAGCTCCGTGGGCA GTGGTACCTTTGGGGGCCTCCAGCAGCAGGATCGCATGGGCTACCAGCTGCATGGATCTGAGGTTAATGG CTCGCTCCCAGCTGTATCCAGCTTTTCGGCTGCCCCTGGCACTTACAGTGGGACTTCCGGCCACACGCCC CCTGTGAGTGGGGCCGCAGCTGAAAGCCTCCTAGGCACCCGAGGGACTACAGCCAGCAGCTCAGGGGATG CCCTTGGGAAGGCACTGGCCTCGATCTACTCCCCGGATCACTCCAGCAATAATTTCTCACCTAGCCCCTC AACGCCTGTGGGTTCACCCCAGGGCCTGCCAGGGACATCACAGTGGCCCCGGGCAGGAGCGCCCAGTGCC TTATCCCCCAACTACGATGCAGGTCTCCATGGCCTGAGCAAGATGGAGGACCGCTTGGACGAGGCCATCC ATGTCCTGCGAAGCCACGCTGTTGGCACCGCTAGCGATCTCCATGGGCTTTTGCCTGGCCATGGCGCACT GACCACGAGCTTCACCGGCCCCATGTCACTGGGCGGGCGGCATGCCGGCCTGGTCGGGGGAAGCCATCCT GAGGAGGGCCTCACAAGTGGGGCCAGTCTTTTGCATAACCATGCCAGCCTCCCCAGCCAGCCCAGTTCCC TCCCTGACCTCTCACAGAGACCTCCCGACTCCTATAGTGGACTCGGGAGGGCAGGCACAACAGCGGGTGC CAGCGAGATCAAGCGGGAGGAGAAAGAGGATGAGGAAATCGCATCAGTAGCCGACGCCGAAGAGGACAAG AAGGACCTGAAGGTCCCACGCACGCGCACCAGCAGTACAGATGAGGTGCTGTCCCTGGAGGAGAAGGACC TGAGGGACCGGGAGAGGCGTATGGCCAATAACGCTCGGGAGCGGGTGCGCGTGCGGGACATTAACGAGGC CTTCCGGGAGCTGGGCCGCATGTGCCAGCTGCACCTCAAGTCGGATAAGGCGCAGACCAAGCTGCTCATC CTGCAGCAGGCGGTGCAGGTCATCCTGGGCCTGGAGCAGCAGGTGCGAGAACGCAACCTGAACCCCAAAG CAGCCTGCTTGAAGCGGAGGGAGGAGGAGAAGGTGTCTGGCGTGGTCGGGGACCCACAGCTGCCCCTGTC AGCCGCCCACCCGGGCCTGGGTGAGGCCCACAACCCAGCCGGGCACCTGTGAGCCGTCACAGCTTCTTCG TTGGACCAGGGACCACCATATCTCTGCCCGGGGTGCATCAGGACGGTTCTGGATGAGACAGGTCTCCATC GAAGCATGAGCAGAGAGAGGGCTCTGGGGACACTTCAGGGCCTGGGGAGGGTGGCACTGAACAGCTCCCT GCTTGGCCCCAGTGACCAAGCAGAAAAGTTCCTTCCTCTCGGTTAACCAGAACTGGAAACAAAGCAGCAT GCTCCCTTTTCAAAAAGGAAAGAAAGATGCCTTAACTATGTAAGACGGAAGAGTCGGACCGTGCCCTGGC AGGGCGGCCTGGGACTGGCTTCTACTTCAGAGCCACCAGCACATCGTGCCTAAGCATTTTTCGTTTTTTT AAAGGAGAATAAAGGAACATTAGTTTTCAGATTTTTTTTTTAAATGTAGACAAAAGTTAGCAAGAACGAG GCCTTCCGTGTCTTTTTTTTTTCCCTTAGCTTTTTTTTCCGTATGTTTTGTAAGCAACAAATTTTTGTAT AAAAGTCTCATGTCTGTTTCTGTTTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAATATTTAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2631291 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2631291 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17266 same embryo
	EMAGE:17270 same embryo
	EMAGE:17267 same embryo
	EMAGE:17269 same embryo
	EMAGE:17268 same embryo
	EurExpress:euxassay_001651 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source