EMAGE mouse gene expression spatial database

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EMAGE:17267

Ube2q2 ubiquitin-conjugating enzyme E2Q (putative) 2 ( MGI:2388672)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001645. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001645_01	euxassay_001645_02	euxassay_001645_03	euxassay_001645_04

euxassay_001645_05	euxassay_001645_06	euxassay_001645_07	euxassay_001645_08	euxassay_001645_09

euxassay_001645_10	euxassay_001645_11	euxassay_001645_12	euxassay_001645_13	euxassay_001645_14

euxassay_001645_15	euxassay_001645_16	euxassay_001645_17	euxassay_001645_18	euxassay_001645_19

euxassay_001645_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17267_wholemount_strong.wlz

17267_wholemount_moderate.wlz

17267_wholemount_weak.wlz

17267_wholemount_possible.wlz

17267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5683
Entity Detected:	Ube2q2, ubiquitin-conjugating enzyme E2Q (putative) 2 ( MGI:2388672)
Sequence:	sense strand is shown >T5683 CGGACGCGTGGGCGGACGCGTGGGGTCGCGGCCGTGACGGCGGCTCCCGGCCCCGGCTCCCTTTCCTCAC CCCTCCCGCTGGAGATGAGGGGAAGATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCC ATCTTCGACAAGAACCACGAGCGATTCCGCATCGTCAGCTGGAAGCTGGACGAGCTGCACTGCCAGTTCC TGGTGCCGCCGCCGCCACCACCGCCGGGCAGCTCGCTTTCGCCGCCGCCGCCGCTCACACTTCACTGCAA CATCACGGAATCTTACCCATCTTCTTCACCAATATGGTTTGTGGACTCTGATGACCCAAATCTGACATCA GTTCTGGAACGCTTAGAAGATACTAAGAACAACAGTTCGCTCCGTCAGCAATTGAAGTGGTTGATATGTG ACCTCTGCAGATTATATAACCTTCCTAAGCACCTGGATGTTGAGATGCTAGATCAACCACTACCCACGGG TCAGAATGGGACAACTGAAGAAGTAACTTCAGAAGAAGAGGAAGAGGAGGAGATGGCTGAAGATATAGAA GACTTGGATCACTATGAGATGAAGGAAGAAGAACCTATTAATGGGAAAAAGTCAGAGGATGAAGGGATTG AAAAAGAAAATTTGGCAATATTAGAGAAAATCAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGT GTCTGGGTCAGTACAAGCATCAGACAGACTGATGAAGGAGCTCAGGGACGTCTACAGATCACAGAGCTAC AAGGCAGGGATTTATTCAGTGGAGCTAACAAATGACAGCTTATATGACTGGCATGTCAAACTACACAAGG TTGACTCTGATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAAGAAAAAGAAGGCATAGAATATATTTT GCTTAACTTCTCTTTTAAGGATAATTTTCCATTTGATCCTCCGTTTGTTAGAGTGGTGTTACCTGTTCTC TCAGGAGGGTATGTGTTGGGTGGAGGAGCACTTTGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTG CCTACTCAATAGAATCTGTCATCATGCAGATCAATGCCACCTTAGTGAAAGGCAAAGCCCGGGTGCAGTT TGGAGCAAATAAGAATCAGTATAATCTAGCACGAGCCCAACAGTCCTATAATTCCATTGTACAGATACAT GAGAAAAATGGCTGGTACACACCTCCAAAGGAAGATGGCTAACTACATTGACATATGTCTGGACCAATGT TGCTTTAAAGAAAATCGTTCCAACGTACAGACATAAGCTTTTGAGTGCCCGTATAACAGCAGTACCGAAG ATATTAGCTAATAGATCATTTAGTGGATAATCTGTCAACTGACATCCAGTTACAGCCTTTTCATTTTGCT CACTTTAGGTATCTTGGACTGAGCAGTGGGGCCTTTACTGTATTTTTCCTGATAAGTACACACAATAGCC ACTCCCTACCACCTCTTTCTTGAAAAGTGAAATCTTTTAAGCAGGGAAGTGAGCATCCGTTTACTGCAGC TGTGATTTTTACAGTAACCTTTCTATATTGAGCCTATGGGGTATGAAGATTTGCAAAATCTTGTTTGTTT AGAGCCAATAAAAGTTTAACTGATGGTCAATACTGGTTTAGAAATTTTAGGTCTTCTAAACCATAGCTTT TTCAGGTCTGAAATAATTTTATTGCCAAAATTATGACAGGAAGCCTCCTCATTAAATTGTTACACTTTTT CACGGTTGTGTGAAAGTTTGAACTAATCTAAAAGTATGATCGTAATTGTACCATATTAGCGTACAGCAAA ACGAGGCAGCCACTTGTGAGCAGTAATTTCCACTGGAGCAGCAGGGTCCTAGTGGACCAAACCTGCTTCC TGTTGGTTCAGCATTTCTTGTGAGGAAGAGCCACAGTGCAGAGCCTGAAGCTCTAAAGTATGAGGTCATT TTACCTTTCCATTTATTAGCGGGGTGTTGCAATTGACGATAAACTAATAAACTTATGGAATGGTTGGCAC AAAGACAGACTCTTTGAGCAAAATTATGAAAGATACCTAAATTGGAGATCCTTAAATTTTTGCTGTAGTC ATGCAGTGGCTTTTACATCAGAAGACCAGTAGGAGTTCAGCCTGTGTTAATTCTTTTTTAAAATGTGACA CAGATCAATGTACTTTGCATTTTTATTACCTTTCCTGAATCAATCCTCTTTGGTTGGTAATAGACTTTTT TGGATCCATGTTTGATTTGAATCTAGCTACTTAAGCACTTTTAAAAGAAACCTCGAAGCACACTCCTGCA CGACCCAGTACAGAGTTAAACGGCTTTTCTTATATATTTGATTTGAGATTTTTTTTTTAAACTTTGCAAA CCACATCTAGGAGATGTGGCACTTGTGCATTTTATGTGTGGTGTTAGTTTCTCTTGATTCTGTTGGTTAA GAGGGCAGAAAAGATATTTGGTATTCTTGGTCACTTGGTTACCCTAAATATATGATTGCCAGTGGCTCAG ATGACAAGTGACAAGACAGAATTTTTCAACACATTATTAAGTTCCTTATGCCTAAGGCAAAATCTTGAAT ACATTATTGAATTTTAATTTCCTAATGGCTAGCAGATTGACAGCTGCACATTTGGCATGCTTTGGGGTTG GGTTTTTTTTTTTTTTTTGATTTTATTTTTCATTGCAGATTTATTTGGCAATGTACAGTAAATTTTGTAA ACTTGCATCAAGTTTATGAATAAAGAACCATTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2631258 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2631258 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17266 same embryo
	EMAGE:17270 same embryo
	EMAGE:17269 same embryo
	EMAGE:17265 same embryo
	EMAGE:17268 same embryo
	EurExpress:euxassay_001645 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source