EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17269

Shmt2 serine hydroxymethyltransferase 2 (mitochondrial) ( MGI:1277989)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001650. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001650_01	euxassay_001650_02	euxassay_001650_03	euxassay_001650_04

euxassay_001650_05	euxassay_001650_06	euxassay_001650_07	euxassay_001650_08	euxassay_001650_09

euxassay_001650_10	euxassay_001650_11	euxassay_001650_12	euxassay_001650_13	euxassay_001650_14

euxassay_001650_15	euxassay_001650_16	euxassay_001650_17	euxassay_001650_18	euxassay_001650_19

euxassay_001650_20	euxassay_001650_21	euxassay_001650_22	euxassay_001650_23	euxassay_001650_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17269_wholemount_strong.wlz

17269_wholemount_moderate.wlz

17269_wholemount_weak.wlz

17269_wholemount_possible.wlz

17269_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17269_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 14 15 16 17

pituitary gland

moderate

regional

moderate expression: see section 09 10 11 14

vibrissa

strong

regional

strong expression: see section 02 03 04 05 06 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 03 21 22 23 24

axial skeleton

moderate

regional

moderate expression: see section 10 11 12 13 14

nucleus pulposus

moderate

regional

moderate expression: see section 10 11 12 13 14

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5679
Entity Detected:	Shmt2, serine hydroxymethyltransferase 2 (mitochondrial) ( MGI:1277989)
Sequence:	sense strand is shown >T5679 GCGTTCTAAGAGCAGCTGGTTTTCGACCCCGGTACTACACCGATACAGAGTTAGTGGCTAGTCCTCCTGT GCCTCCTGTAGCGATGGTATCCTTCTCTTTGCTTCGGACCACTCGGCCTCTGCAGAGATGTGGCCAGCTG GTCTGCATGGCTGCCCGGGCCCAGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAACTGGAGGTT GGACGGGCCAGGAGAGTTTATCAGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAG ACAGTGTCGCGGCCTGGAGCTCATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGG TCCTGTCTCAACAACAAGTACTCGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGG ACGAGATCGAGCTGCTCTGCCAGCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGT CAATGTGCAGCCATACTCAGGGTCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGAT CGAATCATGGGGTTGGACCTGCCCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGA TCTCCGCCACATCCATTTTCTTCGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTA CGACCAGCTGGCGCTGACCGCTCGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCC CGCCTCATTGACTATGCACGCATGAAAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGG CCCACATCAGTGGCCTGGTGGCTGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCAC CACCACTCACAAGACACTGCGAGGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTA GACCCCAAGACTGGCAAAGAGATCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCC TACAGGGTGGCCCCCACAACCACGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTAT GTTCCGCGAGTACTCCTTACAAGTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGA TACTCGCTGGTGTCTGGTGGCACTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATG GAGCCCGAGCCGAACGCGTGTTGGAACTTGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCG GAGCGCCATTACTCCGGGGGGCTTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGAC GACTTCCGTAGAGTCGTCGATTTTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTG CCAAGCTCCAGGATTTCAAATCCTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCG GCAACAGGTGGAACAGTTTGCCAGGGGCTTCCCGATGCCTGGATTTGATGAACGTTGAGGACAGTTGAGG ACACCTGGCGGTCGGTCAGAAGCCCACAGACCCGGAAGTTACCTTTCTTCCCGCCTCCCTGGACCATAGA AGAGATGTCACTCCTCTCTGGGTTTTAGTGGAGGGAGGGAGGCCTTCCATTTAGGGCAAGAGCCAGGCAT AAACTCCCTTCCAGAATTTGTAGCTGAGATTTCTGATCTTTAACCGTGTCTACACGAAGCACCCAGTTGA CATCCGACTTGTTTTTGAGACGGGGGTCTCACTTAGCTGTGAGTGGCCTTGAACTGACCCTTCCACCTTC ATTTCCCAAGTGCTGGGGTTATAGGTTTGCACCATGACACCCGACTTCGCCCTCAGCTTTCCACCCCGAC CCTCCCACTACATCTCAAGTGTTACAGACTCCCCTCCCGCTCTTTCTTGGGGAAGAGGGGATGGGTAAGC GCCCTCCTTCCTGTTTTTATCTAATAAAATGCTAACCTGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2615850 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2615850 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17266 same embryo
	EMAGE:17270 same embryo
	EMAGE:17267 same embryo
	EMAGE:17265 same embryo
	EMAGE:17268 same embryo
	EurExpress:euxassay_001650 same experiment
	MGI:4828062 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source