EMAGE mouse gene expression spatial database

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EMAGE:17279

Tcea1 transcription elongation factor A (SII) 1 ( MGI:1196624)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003691_01	euxassay_003691_02	euxassay_003691_03	euxassay_003691_04	euxassay_003691_05

euxassay_003691_06	euxassay_003691_07	euxassay_003691_08	euxassay_003691_09	euxassay_003691_10

euxassay_003691_11	euxassay_003691_12	euxassay_003691_13	euxassay_003691_14	euxassay_003691_15

euxassay_003691_16	euxassay_003691_17	euxassay_003691_18	euxassay_003691_19	euxassay_003691_20

euxassay_003691_21	euxassay_003691_22	euxassay_003691_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17279_wholemount_strong.wlz

17279_wholemount_moderate.wlz

17279_wholemount_weak.wlz

17279_wholemount_possible.wlz

17279_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17279_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5667
Entity Detected:	Tcea1, transcription elongation factor A (SII) 1 ( MGI:1196624)
Sequence:	sense strand is shown >T5667 CCCACGCGTCCGGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCT TCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGCCA AGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAA TATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAG AGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGAC CATCAACTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGC AAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATAT GTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTG CAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGA GGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCA AATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTAT TTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGGAAAAACCTGACCAAAGA AGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGT AAAAAGAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCAATGACAACATTTGTTG TATGTAATGAATGTGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATCTGGACCAT TAAGAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGGCAACTCGTTTCCTTGCAAGTGAAATTT GTAAACAACATACATCTCATGGGTTGGTCTTTGTTGTTCACCTGACAGTCTGTCTTAAATGCCTTCTGTG GTCTCAGATCAGCTGGGAGACCATAAAATAATGATATAATGTGTTGCTTTGTTTTTCTCTTTCATAAGTT GATGTTGCATTTTATTAAATATTAACTTTTTATAGCCTAGAACACAAAAATTTTGTTGATCTGTTAATGC ATAAAGATAAATTGCTTTTCTCATTGGTATGTACCTAAACATGTTAAAAGGAAAAGGCATAATATAAATT TTTAGAGTTACCAAATGTAGTGTGTATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATT TGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAGCCTCGGATCCTTGGATCTCTTGCATC TGTGACTAACCACAGTGATCATTTCTTTTATTTCTCATACATATATAATACCATTTTTTAATAGATTTTA CTCTGAAAACGGACTTGGTTTATTTTATTTGACTTTGTGCTATATTTTAGTTTTATAGAACTCTGTTATA ATTTCCTAAGCTTTTCATAAAGCCCACCACAGGTATACTTCTGATGAATTGTCCCCAATAGGAAGACATG CTATGAAGAAAATGTTAGTATCTTAGTAGAGTTCCTGGAGGCAGCATGACTGTACTTGCACTTGGAAACC TACTGACCAAGGATTAAGCCTGAGAACTGTAAATCATTAAGCTATGCTCTTTGTGTATGGAGGTTGCAAA AAAAGGCACCTCCTTTAGACTGAATCTTTTGTGCTGAAATTTTTCTTACATGATGATGTCAAGCCAGCTG AAATGTAGGCTGTAGCAAATAGTTTTACAAAACATATTTCACAATTAAGTTTTACTTTTTAAATGTGTCC CACTTGTTTTGCTAGTAATAAGCAGTGGTTTTCACATCGTCTTCCTGACACAGATGTTGTAATGCATATA GTAAAGGCCACTTTTATAAATTAAGTGCTTCTGCCTGTGTTCAGACTCCTGTGTAAAAACCGTTGCACAA GCTTTGCACTCTTCTATGCTCTGCTTTGTAGACCGTTTGTCATAACATGGAAAACAATATGTCCATGTTA AAAGGATTGGTCTGCCGGGCGTGGTGGCCCACACCTTTAATCCCAGAACTCGGGAGGCAGAGGCAGGCGG ATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACC CTGTCTCAACCCCCCTCCCCCCCCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAAATA AAGTACAGTTTGAAAGAATTAGTTTAAAAGTAATAAGGTTAAAATAGTCCCACTTTTAAGTGATCCATTT TAAAATTTGTAAATCAATAAAGTTTTTTGTTGTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2582185 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2582185 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17278 same embryo
	EMAGE:17277 same embryo
	EurExpress:euxassay_003691 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source