EMAGE mouse gene expression spatial database

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EMAGE:17282

Dnajc3 DnaJ (Hsp40) homolog, subfamily C, member 3 ( MGI:107373)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003682. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003682_01	euxassay_003682_02	euxassay_003682_03	euxassay_003682_04

euxassay_003682_05	euxassay_003682_06	euxassay_003682_07	euxassay_003682_08	euxassay_003682_09

euxassay_003682_10	euxassay_003682_11	euxassay_003682_12	euxassay_003682_13	euxassay_003682_14

euxassay_003682_15	euxassay_003682_16	euxassay_003682_17	euxassay_003682_18	euxassay_003682_19

euxassay_003682_20	euxassay_003682_21	euxassay_003682_22	euxassay_003682_23	euxassay_003682_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17282_wholemount_strong.wlz

17282_wholemount_moderate.wlz

17282_wholemount_weak.wlz

17282_wholemount_possible.wlz

17282_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17282_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5693
Entity Detected:	Dnajc3, DnaJ (Hsp40) homolog, subfamily C, member 3 ( MGI:107373)
Sequence:	sense strand is shown >T5693 GAGCGAGCGAGCGAGTGGACTGGGCGCTTCGCTGCGGGCTGTTGGCCGCGCGGGCTGCTGGGCCGCGCAC CTTCCTGCTCTTCGGCTCAGGCGCCCTTGGACATGGTGGCCCCCGGCTCGGTGGGCAGCCGGCTGGGCGC GGTGTTCCCGTTCCTGCTGGTCCTGGTGGACCTGCAGTACGAAGGCGCTGAGTGTGGAGTAAATGCGGAT GTGGAGAAGCATCTTGAATTGGGGAAGAAATTACTCGCAGCCGGACAGCTAGCCGACGCCTTATCACAGT TTCACGCTGCAGTTGATGGTGACCCCGATAACTATATCGCATACTATAGGAGAGCGACCGTCTTCTTAGC CATGGGGAAATCCAAAGCAGCCCTGCCCGACCTCACCAAAGTGATTGCGCTGAAGATGGACTTTACTGCC GCAAGACTACAGAGAGGTCACCTACTGCTCAAACAAGGGAAGCTTGACGAAGCAGAAGACGATTTCAAGA AAGTGCTCAAATCTAACCCCAGTGAGCAGGAGGAGAAGGAAGCCGAGTCTCAGCTTGTAAAAGCTGACGA GATGCAGCGGCTGCGCTCCCAAGCGCTCGACGCCTTTGACGGTGCCGATTACACTGCTGCTATAACCTTC CTTGATAAGATTTTAGAGGTTTGTGTTTGGGATGCAGAATTGCGTGAACTGCGAGCAGAATGCTTCATAA AGGAAGGAGAGCCCAGGAAAGCCATCAGCGACTTAAAGGCTGCTTCAAAGCTGAAGAGTGACAACACAGA GGCGTTTTACAAAATCAGCACACTCTACTACCAGCTAGGAGACCATGAGCTGTCTCTCAGTGAAGTTCGT GAATGTCTGAAACTTGACCAGGATCACAAGCGGTGTTTTGCACACTATAAGCAAGTGAAGAAACTTAACA AGCTGATTGAGTCAGCTGAAGAGCTGATCCGAGATGGCAGATACACAGATGCAACCAGCAAATATGAATC AGTCATGAAAACAGAGCCCAGTGTTGCTGAGTACACAGTGCGCTCGAAGGAGAGGATCTGCCACTGCTTC TCTAAGGATGAGAAGCCCGTGGAAGCCATTAGGATATGTTCTGAAGTTTTACAGATGGAGCCCGACAATG TGAATGCTCTGAAAGACCGAGCCGAGGCCTACTTAATAGAGGAAATGTACGATGAAGCCATTCAGGATTA TGAAGCTGCTCAGGAGCACAATGAAAATGACCAGCAGATTCGGGAAGGCTTAGAGAAAGCCCAGCGGTTA CTGAAGCAGTCACAGAAACGAGATTATTATAAAATCTTGGGAGTAAAAAGAAATGCCAAAAAACAAGAAA TCATTAAAGCATACCGAAAGTTAGCACTGCAGTGGCATCCAGATAATTTCCAGAATGAAGAAGAAAAGAA AAAAGCAGAGAAAAAGTTCATTGACATAGCAGCTGCTAAAGAAGTCCTCTCAGACCCAGAAATGAGAAAG AAGTTTGATGATGGAGAAGATCCTCTTGATGCAGAGAGTCAACAAGGAGGTGGTGGCAACCCCTTCCACC GAAGTTGGAACTCATGGCAAGGGTTTAACCCCTTCAGCTCAGGCGGACCGTTCAGATTTAAGTTCCACTT CAATTAAACCAGCTATTTTACTGCTCCTCTTCTTTATTATTATTTTTAACATTAAAAACAAATTTTGTTT GGGATTCTAATGGAAACAAAATGGAACAAATCTTTTAGTTTGTCCATGACCAAAGAGTTGTTTTAAAGAA AAAAAGCTGAGCTTATTGTAGACTTAAGGTTGATGGGCCTGTCAAGGTGGGAAGCAAGGAGCTAACCGTA TTTCTGACGAAGCAGCCTATACTAATTTGTGACTTCTGGGAGAAGTGGTCTGAGATACTTATCCTGTGGA GGTGTTCAGAAAAGCGTTAAATAGACATTGATAACCCTGGAGCAGACAGATTCTATGCAGGCTGTGTATT AACCGTGAGGAGGAGCTGTGATCTTTGGTGGATTGTGTCTCAGTATTCTTAAAGGAGTGTGGACAGCTGA GCTTCATTTTTGGAGGAACAGTTATGCTTAGACTTTGGTCAGTCCATTCACTTGCAAGTATTTCTGTACC AGTGTCCTCAGCTCTCACTGCAGGAGGGGAGAGACCGTCTTCCAGGAAACTTACCAGCAGGTGGTAGGCT GTATGTCCAGGTATAGACTGGAAGTATTTTTGTTGTTGTTTTATATCTATAGGAGAATACCAATTTCACT TGAATTTAATTGTTAAACTAAACAGAGTAAGTTAAAGTATATGAAAGTGGCTCTGTGGTTCTCTAGACAA CACGGTTGAAAGTCAGTTTGAAATGCTTTTTAAGAAAGTGAAGTTCCAAGTTGTTTGGTCGAAATTCCCT GTCACAAAATTGGTTAGAAGTGGAAAGTTGCTCTGTCAACATGGGCAGAGCATTATGGTTGCCTCTCGCA AGCAGTGCGCTGAGGCAGCGTCTCCTTCTCTCACACATTTGTCCAGCTGTCTCTGTTGTTTTCCTTGCTG GGTTTTGGACTGGCTGTTCCCTGGCTGTTTCTTCTGTGAGAAGAAAGTAGAGGCTTGCAAATCGATTCAA CTCTGGCTGTGTACCTTCTATCATTCACTTAATAAGATGGCACTTCCTCCTGAGACATTCCCGTCCCTCT TACTTTAAAGCAGATACTTAAGAAATGTACTAGTCAGTATTTGTATACTGTATAATTTTTGCCTTAAAAA TCTGTTGCTAAACAAGAATATTGAATACTTATGGAACTACTGATGCTGTGTTGAAAACTTAGAAGAATTA CTCTGGTAAGAATTATTGCCTCTATTCTAGAAAAAAAAATTTTAAACATTGTGAGATATTTTAAGCATAA AGGTCTAATATATTGCTTTTATGAAATTAAACATTTTAGTTAATTGTGGGAAGGAAGCATGAATGTAGCA GCCAAGTACAAATCATTTTGTTAACCTATAGTCATATCAACTTGAGCAGATATAGAAAAAGATTTTGTTA ACTGCAAGAACATGAATTACATAAGTTGAGTTGGGCAAAACATTTTAAGTGCTTTTTGCCTCTACTTAGG AGCCTTTTTCTTACCAAAACACTTAACAATGCATTTCAAAATACCTTTCAGCTTTCATTTTGCTTCAGTG TCTCACAGTTCAAGGCAGGCAAGAAAACTCACAGCTTTTATCCTAGCTGTAGAGACAGGCAGAGGCAGGT GGATTCCTAAGAGTTTGAGCCAGCTTGGTTTACATAGTGAGTTCCGTGCCAGGGCCTCCACCAAAATATT AAATCAATTCAAGCCAGTGTGAAACATTGAATGAAAAATAAAAGAACATTTTTGTGTGTTTTTCAGATCT TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2646147 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2646147 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17280 same embryo
	EMAGE:17281 same embryo
	EurExpress:euxassay_003682 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source