EMAGE mouse gene expression spatial database

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EMAGE:17292

Lrrc59 leucine rich repeat containing 59 ( MGI:2138133)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003680. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003680_01	euxassay_003680_02	euxassay_003680_03	euxassay_003680_04

euxassay_003680_05	euxassay_003680_06	euxassay_003680_07	euxassay_003680_08	euxassay_003680_09

euxassay_003680_10	euxassay_003680_11	euxassay_003680_12	euxassay_003680_13	euxassay_003680_14

euxassay_003680_15	euxassay_003680_16	euxassay_003680_17	euxassay_003680_18	euxassay_003680_19

euxassay_003680_20	euxassay_003680_21	euxassay_003680_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17292_wholemount_strong.wlz

17292_wholemount_moderate.wlz

17292_wholemount_weak.wlz

17292_wholemount_possible.wlz

17292_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17292_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5710
Entity Detected:	Lrrc59, leucine rich repeat containing 59 ( MGI:2138133)
Sequence:	sense strand is shown >T5710 GGAGTGCGGCGGGCGAGCGGACGGGCGGCGGAGGCCTCGCAGCGGCGGCGGAGGCGACGGCGGCTTAAGG CGCAGGCGTGGTCCGTTGGGTGTGAGTCCGCCGAGCCCACGAGCGGCCCCTCATCCCTCTCTCCTGCCCG TCGGAAACCGGGAGCCAGGGACCCGCTTAGCCGGCGTCATCATGACCAAGGCCGGTAGCAAGGGCGGGAA CCTCCGCGACAAGCTGGACGGCAATGAGCTGGACCTGAGCCTCAGCGACCTGAATGAAGTCCCCGTCAAG GAGCTGGCTGCACTTCCCAAGGCTACGGTATTGGATCTGTCCTGCAATAAACTGAGTACTCTTCCGTCGG ATTTCTGTGGCCTCACACACCTGGTAAAGCTGGACCTCAGCAAGAACAAGCTGCAGCAGCTACCCGCAGA CTTTGGCCGCCTGGTTAACCTTCAGCATTTGGATCTCCTCAACAACAGGCTGGTCACCCTGCCTGTCAGC TTTGCCCAGCTCAAGAATCTGAAGTGGCTGGATCTGAAGGACAATCCCCTGGACCCTGTCCTGGCCAAGG TGGCAGGTGATTGCTTGGATGAGAAGCAATGTAAGCAGTGTGCAAACAAGGTGTTACAGCACATGAAGGC CGTGCAGGCAGATCAGGAACGAGAGCGGCAGCGCCGGCTGGAAGTGGAGCGAGAGGCAGAGAAGAAGCGT GAGGCCAAGCAGCAAGCTAAGGAAGCGAAGGAGCGCGAGCTGCGGAAGCGGGAGAAGGCGGAGGAGAAGG AGCGCCGGCGGAAGGAGTATGACGCTCAGAAGGCGTCCAAGCGGGAGCAAGAGAAGAAGCCTAAGAAAGA GGCAAACCAGGCCCCGAAATCGAAGTCTGGCTCTCGTCCTCGCAAGCCACCACCCCGAAAACACACTCGC TCCTGGGCTGTGCTGAAGGTGTTGCTGTTGCTCCTGCTGCTGTGTGTAGCAGGAGGGCTGGTTGTATGCC GGGTGACAGGGCTGCACCAGCAGCCCCTCTGCACCAGCGTGAACACCATCTACGACAATGCGGTCCAGGG CCTGCGTCATCATGAGATCCTCCAGTGGGTCCTGCAAACCGACTCCCAGCAGTGAGCTCACCCTCAGCAC CCGCTGCCTCCCAGCCTTGGAGCTTGGATTCCTATGGAATTGGGTTCTGCTGGACACAACCTCTTTTTAG CGTCAGACCTACCTGCCATCATCACATGGCTGCTGGTGGTACTTGAGATCTCCCCTTTGTAGGACTTCTG TTCCATAGTCAGGGTTCCCTGGTGGAATGAGGAGAAATGGAGAGGGGGGAGGAAGAGTTACCTGCATGCC TAAAGGAGTAGGCTTAGGGGTGGGGAGAGAGAAGGCATAGGCTTTTCTAGTTATACAAAGCTGTGTAAGG CAAGGTTCCTTTCTACTAAATGGTCAGCTGTCACTACATTTATACTTTTGTATGTCATAAACCCTTTCTT TCATTCCTCCCTGGGTAACCAGGACAATCGGAGGGCAGTGTGTTACTGGGATTAGAGGACTAGCAATACT GGGTAACCCAGCCTAAGCTGGGAAGGTGACGTAATACGTTTCTTTAAAGATTCAGTCAGTCAAGCAGTTT AGCAATATCCAAAATGTCTGGCTGTTTGGTCCAGTGTCACATGTTGAGGCAGGAGGTGCCGTCTCATCCA TGACTTGCCAGCAGTGTTCAGGCTAGTCTGAGGCACAACCAGGCTCTCATTCCAGTTTTCCTCCTTCCCT TTATGTCATTCGACCTCCTATATAATACCCAAGAGGATGAACTCACACCAGAGTTGTCTCAGCTAAAGCG AATCTTTCATAACAGTCTTACCACCCACAAATAGATCTCATATCATCAGGGTCCTGGGAAACTTAACTCC TGTGGAATTTTGCCTCAACTTTTAAATGGCTTCCACAAAATGGCAGCAGGCCGGGTTCCTTGCCTCAGTT TTAGAGCATTAACTCCCGAATGGCCTGGAAGCAGAGGGAACTCTGCAAAGCCGTACTGTGGCTGCTCCAC TAGCACTCGGTGTGATGAAATACCTCAAAGGCAACCTAGAAACTTGACTTCATGGAAGCAGGTTTAGAGT TGATTCTGGACCTGTAACAGAAGGGAAGGAAGGAAGAGAACAGTGGGAGTCAAAGGGAAACAAAGTCACA TGGTGGCGCTGCAGAGTGATACATAACTAAACATTAGCACAACCAGGGCAGAGCCGCCCACCTCCCTGCT GCTACAAGAAAGCATTCTCCCCTTTCCCAGTCTCTTCACAACAGCTGCATGAAGGGATCAGAAACAACTG TGTCTCGGTGCTTACTTGCTAAAAACTCCCATTTGCAAGCTCCCTAGAGGAGCAAGGACCTGTCATAGTT CAGCAGTGTAGCCCTAGTGGCCCATCTGGATAGGTCTGCCATTCAAAACTGTCCTGACACTGGAAGAAAG GCCTTGGATTTTTCCTCCCCAGACAGTTCTGCCATGTAGGCCCACACCTCCTCAGCATCACCTCCTCAGC ATCACTACACGCTCCTTTAGTCTTCCTCCAAGCTCCACAGCCATTGGTACAAAGGCTTTATTGAGACAAA ATACATACTACATACATATGGTGACATCATGAAAACAGGAGTCAGCCTCATCATAGCTCCCTAGCTGGTT GAGGCAGCTCAGTGGCTGGGCGTAGTCAAGCCAACCCGCAGGCAAGAGTTCACTCTGACTTCGAGATTTG ATGCTTATTTCTTGGATTTCTACAATTATTAAATCCATGTCTGAGTGGTCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2647513 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647513 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17291 same embryo
	EurExpress:euxassay_003680 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source