EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17322

Nt5m 5',3'-nucleotidase, mitochondrial ( MGI:1917127)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003747. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003747_01	euxassay_003747_02	euxassay_003747_03	euxassay_003747_04

euxassay_003747_05	euxassay_003747_06	euxassay_003747_07	euxassay_003747_08	euxassay_003747_09

euxassay_003747_10	euxassay_003747_11	euxassay_003747_12	euxassay_003747_13	euxassay_003747_14

euxassay_003747_15	euxassay_003747_16	euxassay_003747_17	euxassay_003747_18	euxassay_003747_19

euxassay_003747_20	euxassay_003747_21	euxassay_003747_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17322_wholemount_strong.wlz

17322_wholemount_moderate.wlz

17322_wholemount_weak.wlz

17322_wholemount_possible.wlz

17322_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17322_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 weak expression: see section 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 weak expression: see section 07 08 16 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 weak expression: see section 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6960
Entity Detected:	Nt5m, 5',3'-nucleotidase, mitochondrial ( MGI:1917127)
Sequence:	sense strand is shown >T6960 GTCCGTGTCCGCGGTGGAGGCTGCGTCTGCCGCGGGCCCCGGGCGAGGAGCGGGGCGATGCATAGGCTGC GCGGCTGCTGTGCGCGTCCCCGCGGTGCCCCGCTACGGGCCGAGCGGAGCCGGGCGAGCAGCCGCGCCCT GCGGGTGCTGGTGGACATGGACGGCGTGCTGGCGGACTTTGAGGGCGGCTTCCTCCGGAAGTTCCGCGCG CGTTTCCCCGACCTGCCCTTCGTGGCGCTGGAGGACCGGCGCGGCTTCTGGGTGTCGGAGCAGTACGGAC GTCTACAGCCTGGGCTAAGCGAGAAGGCGATCAGCATCTGGGAGTCAAAGGATTTCTTCTTTGAACTTGA GCCTCTCCCTGGAGCCGTGGAAGCTGTGAAGCAGATGGCCAATCTACAGAACACTGATGTCTTCATCTGT ACAAGCCCCATCAAGATGTTCAAGTACTGTCCCTATGAGAAGTATGCCTGGGTGGAGAAGCACTTTGGCC CTGACTTTCTGGAGCAGATTGTGTTGACCAGAGACAAGACTGTGATCTCAGCCGACCTTCTCATAGATGA CCGGCCAGACATCACAGGGGCTGAGCCACATCCCAGCTGGGAGCACATCCTCTTTACCTCCTGCCACAAC TACCACCTGCAGCTGCAGCCCCCTCGCCGCAGGCTGCACTCATGGGCAGACGACTGGAAGGCCATCTTGG ACAGCAAGCGGCTCCGCTGAGGACTGTACTATGGCTCTCTCGTGGTTCAGCATGGGGCTCACAGCTCACA ACTGGACAGCAGGCAGCCCACTGAGGGTTATGCTGCAGTTCCCTGGTACTTCCTGGAGGATCCCAGTGAA CACTTTGGTCAAAACAACGTCTGACACACTTCCCTTCTTCCCCTAGCCCAGCAAGGCCTTAACCTGATAG TTGGGACGGGGCTAGAAGAAAGATGCGTGTTCTCAGATAAGTTGGTCTGACCTCAGGCAGATGCCCCATG CCACTGCCTCTCACTGTGTGCAATAGGGGTTCTCTGAGACATGGAAGTACCCTGATCCAGGATCTGCAAG ACGAGGGGTGCCAGGGACAGACAGATGACAACGGTTCCCCTGGCAGGCATGTAGGTACCTCTACTGTAGA ATCTTGTCCAGCTTACATACTTAGTCTTTCTGGATTTCCATGGCCACCAGGCTCTTTTGGAGGCCACCGA GACCCTCCCTACGGTCTCTTGCTCTGGTGTTCTTAGATCCCATTAACAGCCCAGAAAGGAGTCAGCAGGT GTGAATATTTGTTGCCAATATGAAAAATAAATGCTACTCACACATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3986525 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3986525 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17321 same embryo
	EMAGE:17323 same embryo
	EMAGE:17324 same embryo
	EurExpress:euxassay_003747 same experiment
	MGI:4826812 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source