EMAGE mouse gene expression spatial database

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EMAGE:17323

D17H6S56E-5 DNA segment, Chr 17, human D6S56E 5 ( MGI:1306799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003741_01	euxassay_003741_02	euxassay_003741_03	euxassay_003741_04	euxassay_003741_05

euxassay_003741_06	euxassay_003741_07	euxassay_003741_08	euxassay_003741_09	euxassay_003741_10

euxassay_003741_11	euxassay_003741_12	euxassay_003741_13	euxassay_003741_14	euxassay_003741_15

euxassay_003741_16	euxassay_003741_17	euxassay_003741_18	euxassay_003741_19	euxassay_003741_20

euxassay_003741_21	euxassay_003741_22	euxassay_003741_23	euxassay_003741_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17323_wholemount_strong.wlz

17323_wholemount_moderate.wlz

17323_wholemount_weak.wlz

17323_wholemount_possible.wlz

17323_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17323_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6959
Entity Detected:	D17H6S56E-5, DNA segment, Chr 17, human D6S56E 5 ( MGI:1306799)
Sequence:	sense strand is shown >T6959 GATAGACTGAGTCGCCCGGGTACCCGTGTTCCCAATAAAGCCTTTTGCTGTTGCATCCGAATCGTGGCCT CGCTGATCCTTGGGAGGGTCTCCTCAGAGTGATTGACTACCCAGCTTGGGGGTCTTTCACAAGCAGTGCC TGGCTGCTGGGAGCTGTTGCTGTAGGGAATGAAAGGTGATGAAGTCTCGATCACTAGACGGGAGATGCTG CCAAGTGCCCCAGACGGGATCAAGACTCCAATGACAACCATAAGATTCCCTAAAGCTAAGACACTGCAGA TCTTGACTCTGCTGATTTCTATAGGTGTGGTCCAAACTAGTCCTATGCCCCATGAACCCCATAACCTTAC TTGGGTTATAACTAACACTGCTACGGGGACTGTAATTAACTCGACCTCCCACCTCGCCCCCATTGGAACT TGGTTTCCTGATCTTGTTTTTGATTTGTGTGCACTAGCGGATGGGACCTGGGATCCCCTGGGCCTTGTAT GGGGGTGTCAACATCCAGCTCAACACAGTGAACTTCGGAGAACCCCCTTCTATGTCTGCCCTGGAGAGGG GAGGTCTCCACAGCAGACGGCTACCTGTGGAGGACCAGAGTCATACTTCTGTGCATATTGGGGTTGTGAG TCGACTGGGTCCATCTCCTGGACGCCACCGATAAAAGATGACCTCATCAAGGTCCAAAGGAGCCCTGGAT CGGAATCCCAAGGATGGGGGTATGGATTGCAGCCCAAGCTGGATGCAAATGATCTTGGTGGGCCATGTTC CTCCAACTGTAACCCCATTACAGTTCAGTTCACCCCCAAGGGAAAAGAAAGCGTAGGATGGGAAAAGGGA AAGACATGGGGACTAAGACTGTATGTGTCTGGATATGACTATGGAGTAATGTTTACGATCCAACTCTCTG CCCGACAGGGCATGCCTTTAGGCCCCAATCCAGCCCTGCCCCCTGTTGGACTCACGGTGACTCAATCACA AATCACAGTCCCCATTTCTCAGGAACTTAGACCCAGGTCTGGGCCATGGGACTTGCTTAAGGCCACTTAC CTGGTATTGAATAATTCTAAACCAGAGCTTACAAGTAAATGTTGGCTCTGCTTGGATGCCCAGCCACCCT ATTATGAAGGAATAGCCGTTCCTGGAAATTATACCACCTCTACTGACAACAAGGACTGTCGTTGGCAGTA TCCAGGTAAAGGGAGACTAACCTTAGAGCTAATTCAGGGAAAAGGACTCTGCTTTGGGACAATACCCCAC ACCCATAGACATTTATGTAATAGCATCCAGGAAGCCCCAGACAGGGATGCTTACATTATACCCCCCATCA ATACATGGTGGGCATGCACTACTGGGCTGACATCTTGCATCCACACCCAGGCTCTTAACCTCACAAATGG ATTTTGTGTACTGGTCCAGCTTGCCCCTAGAATTCTTCGTTACTCTGATGAGATGGGGACCAGACTATCT CCATTCCCAGCTGCTCGAGCGAAGCGGGCTACTGTTGCCGAGATCCTGGGTGCATCAGCCTTCGACTTCC AAGATGAGAATTATAAAGTGTTAAGCTCTGCCATAGATGCAGATTTAATGGAACTAGGGAAATCCTTATC CAAGTCCAAAACCTCATTAACTTCTCTAAGGGAGGCCACCCTTCGAAATCAGCGGGAACAAGACTTCCAA TCTCTGCAACAGGATGGACTGTGTAAACCTCTGGAGAAAAGGTGCTGCACCTTTGTGGATAACTTAAAGC ATGCGAGGGAGTTATTGGCTAAGGTGAGGAAACGTCTAGAAGACAGGAAAAGGGAAATGGAACAGGGTCA GCCTGTTTGGATTACAGTTATTGCAACTCTGTCTGGATTCTTGGGCCTGGTCATACTCATTGTGATATGC AAGCTCTATGTTGTTAACCCCCTGATGGATTCATCAAGGACCAGGTCTTCACCATCCGACACGTATTAAA ATGCTAACCCTTGGATTCTGCTCAAGGGTTTGAGATGGACTCTTAAGGTGAGAGAGAGTGATCCTAACTT ACTCCATTTTAAGACAGGCTGCTAGGTCAGGCCTTAAGTTTAGTCTCAGAAAAGCCATAAAGAATGGGCA GCCAAGCCAGGCGTGGTGGCACACACTTTTAATCCCAGCACTTGGGAGGCAGAGGCAGACGGATTTCTGA GTTCGAGGCCAGCCTGGTCTACAGTGAGTTCCAGGACAGCCAAGGCTACACAGAGAAACCCTGTCTCGGA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985942 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985942 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17321 same embryo
	EMAGE:17324 same embryo
	EMAGE:17322 same embryo
	EurExpress:euxassay_003741 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source