EMAGE mouse gene expression spatial database

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EMAGE:17344

Ccdc21 coiled-coil domain containing 21 ( MGI:1917262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003788. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003788_01	euxassay_003788_02	euxassay_003788_03	euxassay_003788_04

euxassay_003788_05	euxassay_003788_06	euxassay_003788_07	euxassay_003788_08	euxassay_003788_09

euxassay_003788_10	euxassay_003788_11	euxassay_003788_12	euxassay_003788_13	euxassay_003788_14

euxassay_003788_15	euxassay_003788_16	euxassay_003788_17	euxassay_003788_18	euxassay_003788_19

euxassay_003788_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17344_wholemount_strong.wlz

17344_wholemount_moderate.wlz

17344_wholemount_weak.wlz

17344_wholemount_possible.wlz

17344_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17344_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7012
Entity Detected:	Ccdc21, coiled-coil domain containing 21 ( MGI:1917262)
Sequence:	sense strand is shown >T7012 CCCAGAGTGAGTGGGGTCGCCGCTCGCTCTCAGGCCTGTTTGTCTCAGGTAACTGATGGCCATGCAAGAA AAATACCCAAATGACAGGTCTCATGCTACTTCACCAGGTTCCAATGTGATTCAAAAAGGCAGTTCTCTGG GAACTGAGTGGCAGACACCAGTTATCTCAGAGACCTTTCGGAGCCGCTTCAGCCGCTGCTCAAGCATTGC TGACAGTGGGGACACAGCCATTGGGACATCATGCTCAGACATTGCAGAGGATTTTTGCAGCTCAAGTGGC AGCCCTTCCTTCCAGCCAATCAAAAGCCACATAACCATTCCAACAGCCCATGTGATGCCTTCTACTTTGG GGGCCTCTCCTGCCAAACCAAATTCTGCACCTTCCGGGCCTTCTTCCGCTAAGCTGCCCTTGTCAGGATT GACTGAAGGCGTGGGGATGACAAGAAATGGAGACTTCGGAGCTGTGAAGCGCTCTCCAGGCCTAGCAAGG GATTTTATGTACCTCCCTAGTGCTGCCGGGGAGAATGGGAGTCAGCAGTCCTGGTTCCCAGCAGTGGGCC ACGAGAGAGAGGGGGAGATGAGGAAGTTCGATGTTCCTAGCATGGAGTCTACCCTTAACCAGCCAGCCAT GCTAGAGACGTTATACTCAGATCCACATTACCGAGCCCATTTCCCCAACCCAAGACCTGATACAAATAAG GATGTATACAAAGTATTGCCAGAATCCAAGAAGGCACCGGGCAGTGGTGCAGTATTTGAGAGGAACGGAC CACATGCTAGCAGTAGTGGGGTGCTCCCTTTGGGACTCCAGCCTGCGCCTGGACTTTCCAAGTCACTATC CTCTCAGGTGTGGCAACCAAGTCCTGACCCTTGGCATCCTGGAGAACAATCCTGTGAACTCAGTACTTGT CGACAGCAGTTGGAATTGATCCGTTTACAGATGGAGCAAATGCAGAACGGAGCCATGTGTCACCATCCTG CTGCTTTCGCTCCATTACTGCCCACCCTAGAGCCAGCACAGTGGCTCAGCATCCTGAACAGTAACGAGCA TCTCCTGAAGGAGAAGGAGCTCCTCATTGACAAGCAAAGGAAGCATATCTCTCAGCTGGAGCAGAAAGTG CGAGAGAGTGAACTGCAAGTCCACAGTGCCCTTTTGGGCCGCCCTGCCCCCTTTGGGGATGTCTGCTTAT TGAGGCTACAGGAGTTGCAGCGAGAGAACACTTTCTTACGGGCACAGTTTGCACAGAAGACAGAAGCCCT GAGCAAGGAGAAGATGGAGCTTGAAAAGAAACTCTCTGCATCTGAAGTTGAAATTCAGCTCATTAGGGAG TCTCTAAAAGTGACACTACAGAAGCATTCGGAGGAGGGGAAGAAACAGGAGGAAAGGGTCAAAGGTCGTG ATAAACATATCAATAATTTGAAAAAGAAATGTCAGAAGGAATCAGAGCAGAACCGGGAGAAGCAGCAGCG TATTGAAACCTTGGAGCGCTATCTAGCTGACCTGCCCACCCTAGAAGACCATCAGAAACAGACGGAGCAG CTTAAGGACGCTGAATTAAAGAACACAGAACTGCAAGAGAGAGTGGCTGAGCTGGAGACTTTGCTGGAGG ACACCCAGGCAACCTGCAGAGAGAAGGAGGTTCAGCTGGAAAGTCTGAGACAAAGAGAAGCAGACCTCTC CTCTGCTAGACATAGCTTTCAAGATAAACAGTCTGTGGAGGAAGCCAATGGAGAAAACCTCAGAGTGGAC ATGGAATCCCAGCAGAAGGAATGTGACTCCCTCCGAAAGATGGTGGAGAGGCAACAGCTGAAGATGGAAC AGCTGCACTCCCAAGTCCAGAGCCAAAAGCAAGAACTGGCACAAGAAGAGGGAATCAACCAGGCACTGAG AGAGGAGGCCCAGCGGAGGGAGACGGCCCTGCAGCAGATGCGCACAGCCGTGAAGGAGCTTTCAGTGCAA AACCAGGACCTGATTGAGAAGAATCTGACGCTGCAAGAGCACTTGCGCCAGGCCCAGCCAGGGTCCTCAT CTTCACCAGACTCAGCCCAGCTGGCCTGTGAGCTGCACCAGGAACTGGCCAGTTGCCTTCAAGATCTGCA AGCTGTCTGCAGCATTGTGACCCAGAGGGCCCAAGGCCACAATCCTAACCTCTCACTACTCCTAGGCATT CACTCCACCCAGCACCCAGGAACGCAGCTAGATCTACAAAAGCCAGATGTGATCAGGAGGAAACTAGAAG AGGTTCAACAGCTGCGCCATGACATTGAGGACTTAAGGACCAGCCTGTCAGACAGATATGCCCAGGACAT GGGAGAAAACTGTGCCACACAGTGAGGAGTGCCAGGGAGGACTTAGGCTGCTGCTGCCTCGCCAGAAGGT TCCTTCCCTGAGAGTTCTGCCTTCTGCTGCTGCACCCAGAGGGGAGAACCTGTATCTGGGCCCAGGGTTG TGAGAAGCCTGCCTGTTTTGCTGTTCTCTGGCTTTGCCTTATTGGGTTATGTTTTGCTTGTTATCTTAAT TCACTCTAAAAGACTGAGTTACTAATTAACCTTTTTGCAGATTTCAACTGATAAAGTCCTCACAAGCTGT TCCCAGCTCTAACCTGGCATGAGAAGCAAACAGGCTGCTGGCCTCCCCTCCATCCTCCAGGACGGATCCA TGTTCATTCCTATCCATACCTTGGTCCTACTGATGCTCTTTTAAGAGAGGCCTAAAGGGTAAAGTAGGAG TAGCAACCTTTTAAGAATTCTCAGGCCCCTTGTATCAGTCTTCTGAGAACTCAGCTGGCTTTCTGGGTTT TCTCAGGTACCTGTTTCCTCAGGGCTGTTGTTCCCACAGAGGCCTCAAGGTTATCCACCCCTCAGCAGAG CCCCTACTATGATCACTGACTTGGGGACCATGGCAGGGCTCCTCGGCCTGCCTGCTGGGCCATCTTTTAC AGGAAACTCTTCTTAGCTACCCATTGTCTGTCCCTAGAACCTTGGGCATGTGGGCATGTGCTTGGGTCCG CTATGTCGTCTAGGAGTACAGACGCCTCTGTGGTTGCCTCGTGGTTCCCGGGTTGGACATGAGTTTCCCC CGTAAGAGCACAGGGTGGCTGTGGCTGCTCAGTCCTTCCTAAGGCTGGGCGCTGTCGCTGTCCTCTGAAC TGTGCCTCTCATTTGGGGTTAGGAGGGTTCCCTTTTCCTGCTCTAACCTTGACAGTTCGGAGCACCTAAG GTAAAAAAGTGTTGAGTGTTTTCTGGTGTTTTGTGTTTCTCTAAGGCCTTTGAGCCTTAGCTTTCTGGTT TTTCTTGCCTCCCAATTTAGGGATTGTCTCAGTTGTATCTGGTTATTTGGCCAGCCCTCAACCTCCTCTA GTTTGGATCCAAGGGCCCTGTATGTATGAGGAGTAAAGTAGTTGTGGGGGTGCAGACAGGACTTCTGCTA AGCCTTGGGGCTGTTAACAGCACCGTGGACCTCCAGGGCCTGAGGGGCCGCTTTGCCAGTGTTTTAACAA GTTTATGTTTTCTTATGGAGTGGCTTACCTTTCAGAGTAATCACACCCAGCTGACCATCCCCTCAGCCTC CGGGCTGCTGCCTCCTTAAAACAGAGCTTCCTGGTTGGTTTCCACCCTGTTATCGGGAGCCCAGCCTCCA TGCTCACGGGTATTTTTCTTCGTAAAGTTTATAAAGTTATTTATATGAATCTTTGTTAAGTCGACTGGTT TGTATTGCCTATTTTGATTCCAGAATAAACCCTTTTCCCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3992639 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3992639 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17345 same embryo
	EMAGE:17347 same embryo
	EMAGE:17346 same embryo
	EMAGE:17348 same embryo
	EurExpress:euxassay_003788 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source