EMAGE mouse gene expression spatial database

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EMAGE:17348

Srpx sushi-repeat-containing protein ( MGI:1858306)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003802. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003802_01	euxassay_003802_02	euxassay_003802_03	euxassay_003802_04

euxassay_003802_05	euxassay_003802_06	euxassay_003802_07	euxassay_003802_08	euxassay_003802_09

euxassay_003802_10	euxassay_003802_11	euxassay_003802_12	euxassay_003802_13	euxassay_003802_14

euxassay_003802_15	euxassay_003802_16	euxassay_003802_17	euxassay_003802_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17348_wholemount_strong.wlz

17348_wholemount_moderate.wlz

17348_wholemount_weak.wlz

17348_wholemount_possible.wlz

17348_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17348_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervical intervertebral disc

moderate

regional

moderate expression: see section 06

lumbar intervertebral disc

moderate

regional

moderate expression: see section 08 09 10 11

thoracic intervertebral disc

moderate

regional

moderate expression: see section 07

otic capsule

moderate

regional

moderate expression: see section 01 02 03 04 11 12 13 14 15 16 17

sacral vertebral cartilage condensation

moderate

regional

moderate expression: see section 08 09 10

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

viscerocranium

strong

regional

Expression in the turbinate bone.

axial skeleton tail region

moderate

regional

moderate expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7015
Entity Detected:	Srpx, sushi-repeat-containing protein ( MGI:1858306)
Sequence:	sense strand is shown >T7015 CGGACGCGTGGGTTGGCCTCCTCCAGCCCCTTAAGTGAGCTGTGCAGCCTGCCCTGCGGGGGAAGCGCCG GCTCACCATGGGGAGCCCGGGACTTCGGCCCGAGCTGCTGCTGCCTCAAGTACTGCCGCTGTTGCTGGCG CTGCTGCACGTCCTGCCGAGCCAGGGCTTCCCAGGATCTGGAGACTCACCACTAGAAGACGATGGAGTCT GGTATTCACACTCTCTATATAAAGACACTCCGTGGTGCTCTCCCATCAAGGTGAAGTATGGGGATGTGTA CTGCAGGGCACCTCCTGGGGGATACTACAAAACAGCCTTGGGAACCAGATGTGACATTCGCTGCCGAAAG GGCTATGAGCTTCATGGCTCTTCCCAGCTCATCTGCCAGTCAAACAAGCGCTGGTCAGATAAGGTCATCT GCAAACAGAAGCGATGTCCTACCCTTACCATGCCGGCAAATGGAGGGTTTAAGTGTGTCGATGGTGCCTA CTTTAACTCTCGGTGTGAGTACTATTGTTCACCAGGATATACATTGAAAGGGGAGCGAACAGTCACATGT ATGGACAACAAGGCCTGGAGTGGCCGACCAGCCTCCTGTGTAGATCTGGAACCTCCTAGAATCAAGTGCC CAAGTGTGAAAGAACGTATAGCTGAGCCCAATAAGCTGACAGTCCGGGTGTCCTGGGAAAGCCCAGAGGG AAGAGATACAGCGGATGGGATTCTTACTGATGTTATTCTGAAAGGCCTTCCTCCGGGCTCAAATTTTCCA GAAGGAGACCACAAGATTGAGTACACAGTCTATGACAGAGCCGAGAACAAGGGAACTTGCAAGTTTCGAG TTAAAGTAAGAGTCAGACGTTGTGGCAAGCTCAATGCCCCAGAAAATGGTTACATGAAGTGTTCCAGCGA TGGTGACAATTATGGAGCCACCTGTGAATTCTCCTGTATTGGCGGCTATGAGCTTCAGGGTAGTCCTGCC CGAGTATGCCAATCCAATCTGGCTTGGTCCGGCACAGAGCCCAGCTGTGCAGCGATGAATGTCAATGTTG GTGTCAGAACAGCAGCTGCACTTCTGGATCAGTTTTATGAGAAAAGGCGACTCCTCATTGTATCTACACC CACAGCTCGAAACCTCCTCTACAGATTGCAGCTGGGAATGCTACAGCAAGCACAGTGTGGCCTCGATCTC CGACATGTCACCGTGGTGGAGCTGGTAGGGGTCTTCCCAACTCTCATCGGCAGGATCAGAGCAAAGATTA TGCCTCCAGCTCTAGCTCTACAGCTCAGGCTGTTGCTTCGAATTCCTCTCTACTCCTTCAGCATGGTGGT AGTGGATAAGCATGGCATGGATAAAGAACGTTATGTCTCCTTAGTGACACCCATGGCTCTCTTCAACCTC ATTGACACTTTCCCCCTGAGAAAAGAAGAGATGATCCTGCAGGCTGAGATGGGCCAGACTTGCAACGTCT GATGTGCTGTTACATTCATGGTGGCTCCTCATCTCTTATTCTATACTACAGTGCCTGTGGGAAGGCCTTA AAATACCTTTGATGTACAGATTTTCATTTGTAATTTTAAAAGTCTATTTTATTATGTGCTTTCTTTGCTC TTAGACATTGGCACAAGGCTTTTTTTAAACTTTGAAATGTTAAAAAAATACGAATATATCTATTCTAACT CTCCTTTTAGTCCATTGTGGCTGATGAAATTAGAAGCTACAGCCACACTCATGCACTCAGAATATTCCTC AGTGTGAAACATGTTTTCCTTTTTCTTTTATTGTTTATATAAGTCTTTTGTTAATAAAATAATTTGAAGC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3993025 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3993025 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17345 same embryo
	EMAGE:17344 same embryo
	EMAGE:17347 same embryo
	EMAGE:17346 same embryo
	EurExpress:euxassay_003802 same experiment
	MGI:4828448 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source