EMAGE mouse gene expression spatial database

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EMAGE:17357

Mfsd1 major facilitator superfamily domain containing 1 ( MGI:1914118)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003821. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003821_01	euxassay_003821_02	euxassay_003821_03	euxassay_003821_04

euxassay_003821_05	euxassay_003821_06	euxassay_003821_07	euxassay_003821_08	euxassay_003821_09

euxassay_003821_10	euxassay_003821_11	euxassay_003821_12	euxassay_003821_13	euxassay_003821_14

euxassay_003821_15	euxassay_003821_16	euxassay_003821_17	euxassay_003821_18	euxassay_003821_19

euxassay_003821_20	euxassay_003821_21	euxassay_003821_22	euxassay_003821_23	euxassay_003821_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17357_wholemount_strong.wlz

17357_wholemount_moderate.wlz

17357_wholemount_weak.wlz

17357_wholemount_possible.wlz

17357_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17357_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7008
Entity Detected:	Mfsd1, major facilitator superfamily domain containing 1 ( MGI:1914118)
Sequence:	sense strand is shown >T7008 TTGCTTCCTGCTCCGTTTCAGGCTGCAAGCTGATGGAGGACGAGGATGGGGAAGATCGGGCGCTGCTTGG GGGCCGCCGCGAGGCGGACAGCGCAGTCCATGGCGCCCCTCGCGCTCTGTCCGCGCTCTGCGACCCCAGC CGCCTGGCGCACCGGCTCGTGGTGCTGTCGCTCATGTGCTTCCTTGGCTTCGGCAGCTATTTCTGCTATG ACAACCCCGCTGCCCTTCAGACTCAGGTGAAACGGGACATGCAAGTGAACACCACGAAATTCATGCTGCT GTATGCCTGGTATTCTTGGCCCAATGTAGTTTTGTGTTTCTTGGGTGGCTTTTTGATAGACCGCATATTT GGAATACGATGGGGCACAGTTATTTTTAGCTGCTTTGTTTGCATTGGACAGGTTATATTTGCTCTGGGTG GTATATTTAATGCATTTTGGCTGATGGAGCTTGGGAGGTTTGTGTTTGGAATTGGTGGAGAGTCCTTAGC AGTTGCCCAGAATACATATGCTGTGAGTTGGTTTAAAGGCAAAGAATTAAACCTGGTGTTTGGACTTCAA CTCAGCATGGCCAGAATTGGAAGCACAGTGAATATGAACCTCATGGGATGGCTGTATGGGAAGATTGAAG CATTACTGGGCTCAGCTGGCCACATGACCCTGGGCGTGACACTTATGATTGGGTGTATAACGTGCATTTT TTCACTAATCTGTGCTTTGGCTCTTGCTTATTTGGATCGGAGAGCAGAGAAAATACTTCATAAAGAACAA GGGAAAACAGGTGAAGTCATTAAGCTGAGGGATATAAAGGACTTCTCGTTACCCCTGATCCTGGTCTTTG TCATCTGTGTCTGCTACTACGTGGCGGTGTTCCCGTTCATCGGGCTGGGCAAAGTTTTCTTCATGGAGAA ATTCAGATTCTCTTCCCAGTCAGCAAGTGCAATTAACAGTATCGTGTATATCATATCAGCTCCCATGTCT CCATTATTTGGACTCTTGGTGGATAAAACAGGCAAGAACATCATCTGGGTCCTGTATGCGGTGGCCGCCA CCCTCGTGTCCCACATGATGCTGGCCTTCACCTTTTGGAACCCATGGATTGCTATGTGTCTCCTGGGGTT CTCCTACTCATTGCTGGCCTGTGCATTGTGGCCAATGGTGGCATTCATAGTTCCTGAACACCAGCTGGGG ACTGCATATGGATTCATGCAGTCTATTCAGAACCTCGGGCTGGCCGTCATCGCTATCCTTGCTGGCATGA TCCTGGACTCGAAGGGATACTTGCTGCTGGAGGTGTTCTTCATTGCCTGTGTTTCTTTGTCACTGCTGGC TGTTGTCTGTCTGTACTTGGTGAACCGTGCCCAAGGAGGGAATCTAAATTATTCTGCAAAACAAAGGGAA CGGATGAAGCTCTCTCATCCAGAGTAAAGCCGTGAGAAGGGATAGCATTCGCGGCTGACTGGAAACCTTC AGGAAGACTTCCTTTTTTAAAACGTCATGTAGAAAAATAATAGAACGGAAAATATATTTATATATGAAAT ATACCTACTTAAAGTGATTCATGCAGACTATCTTAGCCTTTCATAACCATGTGTTGTGGAAGGTTTCTGG TCGTGAACAGTGAACAGTTTAGGGAATCTGGAACATTCAGGCAAAGACCTCTGTAAGGATCTGCATCTTC TGCCGGGACGATTTCTAATAAGGGCGTCCTGCAGATGTAGCCTTTCCTTTGTATTATAGCAGGAAAAAGG GCAAAGCCACAAAACAGGAGGGTCTGTGGTAGAAGGCTGTTACATACATAATAAAGAATGACATTTTAAC CGCACTCACTGTGTCCTAGAGAACAATATGTAATCGCTCCATATTTCCCTCTAGGAGATTATGTGTGAAT GTCGTTAGTGATGGCTGCTGAGCAGCAAACCTTTGGAAGTTTTTTTTTTTTTTTTCCTTAAAGACAATAA AAGTGTCCTTCAGTTTATGATGGCTACCTATAGACATGGATCCATAAAACCAATGCAAAAGGTAGACTCG ATGCCTGCCTTATATTTTTACAACATTGCAATACCAACTTCAAGTTTTCTGTGGAATATAATCTCCAGAA ATCAAATCTTCAATAAAGAAAATATCCTAGATCCAATCTCATTGAATAGTTTTATAGGTGTAAGGTACGT GGGGTACATAGCCTTGTATGCTTCAAGCTTTGGCACCAAGGGCTGGAGAGATGACTCGGTGGTTAAGAAC ATTTACTGCTCTTTAAGAGGACCCCAGGTCAGTTTTCAGCATCCACATGGGGCCACTCATAGTCACCTAA CTCCAGCTCCCAGGGATCCAGTGCCCTGCTGAATCTTAAGCAGTACAGAAACAGTTTGAAAATGCATTCC TTTTCTTTTTGGTTTATAGTTTGTTATGCATTCTTCACTTGTGAGCTTCGAGGCTTTGACTACATGTGAC TCTCATAGCACATGTCAAGTATATGAACTGGCCCCTCTGCACTGGTCCTGTCTTCCCACTGTGTCCACAT GAGGTGTGGGGAGCCTTGACATGCTTATGAATAGGCTGGGTGCCTGCTCGGCTCACAGGGTTGTCCATCT TGATTTCCTTCTCTTGAGTGTATTCTCTAGTGCACACTCTAACCTGGTTCCATCTGACTTAAACAAAAAC CTTAGTTTCCAGCTCAGAATCATCCTAACATAGAAACTTCTCTAGGCCACTCAGGCTGTATACAATTAAT GATACCTATGAACTTTGAAAGCCTCATCCTGTGCCTTTTTAGCTCACTTCGATTTGTCAGGAATTTGTGG CATTGTGGGCTTGGGGTGTATTTGATCGTCCTTAGATTGTCAGCTCCATGAGAGCAGGGACATTGCCTGG CTTGTTAGTGACTCTATCCTAGTGCCTAGCATAATGAATGTACAAAACTTTTTCTTTCTCCTTTTGTATA ATGTATAAAATAAAAAGTAATTGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3992247 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3992247 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17355 same embryo
	EMAGE:17356 same embryo
	EurExpress:euxassay_003821 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source