EMAGE mouse gene expression spatial database

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EMAGE:17364

Iqcg IQ motif containing G ( MGI:1916957)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003706. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003706_01	euxassay_003706_02	euxassay_003706_03	euxassay_003706_04

euxassay_003706_05	euxassay_003706_06	euxassay_003706_07	euxassay_003706_08	euxassay_003706_09

euxassay_003706_10	euxassay_003706_11	euxassay_003706_12	euxassay_003706_13	euxassay_003706_14

euxassay_003706_15	euxassay_003706_16	euxassay_003706_17	euxassay_003706_18	euxassay_003706_19

euxassay_003706_20	euxassay_003706_21	euxassay_003706_22	euxassay_003706_23	euxassay_003706_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17364_wholemount_strong.wlz

17364_wholemount_moderate.wlz

17364_wholemount_weak.wlz

17364_wholemount_possible.wlz

17364_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17364_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 weak expression: see section 11 14 15

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 weak expression: see section 14

diencephalon roof plate

moderate

regional

moderate expression: see section 12

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 16 17 18 19

4th ventricle lateral recess

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 12 13 14 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 weak expression: see section 12

pons ventricular layer

strong

regional

strong expression: see section 11 12 weak expression: see section 13 14 15

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 moderate expression: see section 10 13 weak expression: see section 07 08 09 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 19 moderate expression: see section 11 12 13 14 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7746
Entity Detected:	Iqcg, IQ motif containing G ( MGI:1916957)
Sequence:	sense strand is shown >T7746 CCAGCTGGGCTGGTAGAGAGGTTCCTTCATTCTTGTGAAATTATAAATCTAGCCCAGAGGGCAAGGAAAG AAACTGAGGAAGAATGGACGAGGAAGAGGTGGAAGTCGTGGAGTCTCCTTCAGAGGTTTTACAACCTGAG GTCACTGTGGTGGTGACAGGAGAGCCTCCTGAAGCTGCAGAAGAAGATCTAGATTACGAAGAAGAGGAAG AGACCTCCCCGGAAGTCATAGAGACGCTGTCCCTTCTAGACGTGCTGAGGGTGTCGGCCGTCATGGAGGA CGTCATTGACCAGCTCTCTATTCTCGGCTACATCATTCCGGTTCAGTATGAAAGAAGGCAGAGCCTCACC CAGAAAGCCAGCCACGAAGGAGCATCTATGATTACAACCACACCGAAAAAATCTACTTCTTTGTTAACTA AAGAAAAATCCATGATGGCAGAAAACAAACAGAGAGGGCAGGACTTTACCTTTAAAAAACCTACAAAGCA GACCATGATGACTCTGGAGACACTGAAGAAAATTCAGAATGACAGGCAATATTTCAGTGATGTAATTGCA AATGCCATGATGGAGATGCAAGATTCTGGCTCTTTCACCAGTCTCCTGAAAGCCTTGGGCAAAGAAAGAG ACAGCAAAATGAATTTCCATGATGTCATCACAAGGGAGGAAAAGGGAAGAAAACAGATAAAAACCCTTCA AAAACAGTTACTTGATGTCAAAAGGGAGCGTCAAATGCAAGTACAGAACGGGAATGAATACATTGCTCAC CTCAGGGACCAGCTACAAGAGATGAAGGCGAAAACCAACTTGGAGAATCTTTATATGAAAAGAAATGCTG AGCTGCAGATTTCCCAGACCCAGAAGAAATGTAACCGAGCAGAAGAACTCTTGCTGGAGGAGATTGAGAA ACTGAGGATGAAAACTGAAGAGGAGAACCGGGTTCACACGGAGATCGAAATGTTTCTTAAAAAACAGCAG CAGAAACTTGAGGAGAAGCTAGAGTTCTGGATGGAGAAATTTGACAAAGACACAGAAGCAAAGCAGAACG AGTTAAATGCTCTCAAGGCTGCCAAGGCCAGTGACCTGGTACACCTGCAAGACCTGGCAAAGATGATCCG GGAGTACGAGCAGGTCATTATTGAGGACCGGATAGAAAAGGAGAAGACCCGGAAGAAGCTGGAGCAGGAT GACCTGGAGCTGAGGAGCATCGTGAAGCTCCAGGCCTGGTGGCGAGGGTCAGTGGTCCGGAAGGAAATTG GCAATTTCAAAATGCCCAAGAAAGATAAAGATGACAGCAAAGACTCGAAAGGCAAGGAGAAGGAGAAGAG GAGAAAGAAGTAACCAGTTTCTGCTGAGCCTTCTCAGGCCTCGAGACACTGGGGGTGCTGACCCAGCGCC CACCTCCTGGCTCACAGACCTAGGGAGTTCTCACTGTCTGATACTCTAGTTTTCACACCCCAGTGAGGGC TATCACATGGCTGTAGGACCTCTTCAGACGGGACTGTGGGGAGCCAAGTTGAGTAGCTGTGTGAGCATCC TTCTTCTCGCTCCAGAAAGCAGCTAACTGGCTCATAGTTAAGTCGTAAAAACTCCTGACACATCTGACTC TGACTTTATGCCATATGATTAAAGTGAAAAACTATTATAAGCAAAAGTGAATTCTCACCTAGCATCATGT GCACGCACATGTAGTTAATAAACATTGGCTCTGCCTGTCTTGCAGGGCGACCATTAGTCTAGGGTTGGTG GTGTTTGGGGGACAGCACATAAAACACCTCTTACATAAGCAGTTCCAGTGACTTTTCTGGGGACAGATAG GGATGGAAGCATCTGATACAAGATGGAAATGGCATGTAGATACACAGTCCTCCAGGGGTAAAGAATGGGG CACGGGTTCTCCAGAGGCTAGTTTAGAGATACACTTGTCAAAGGAGACACAACTATGTCCATCATCTGGA TTTTAAGACGTAAAGAAACCAACCAAACCAACCAACCAACCAACCAACCAACCAACCAACCAGTGTATAT GTGGGCCCAGGCTTGGAAACGTGTGCATGCCACAGTGTGCAGGGGGTGGAGTCAGAGGACAGGTTCCAAG AATTGTTTCCTTCTAATGTATCTCTTTGTACTATTTGGGTCTCAGATATCAAACTCAAGTCATTGGACTT GGCAGCAAGCCCCTTTTCCTGCTGAGCCATTTCATTGGCCCTCAACTGTGGTTTTTTTTTTTCCCCTGAG AGAAATGTTACCTTAATGTGTCTCACATATTTCATGAATATAAAATTTAAACTTTTTGATGCAGGGTCTC ATGTGGCCCAGGTTGGTTTCAAATTCACTACGTAGTCTAGTGGCCTTGAACCTCATGTTGTGCCTCCTAA GTGCTAGAACTACAGGGGCGAGTGACCATACCTGGTTTATGCAGCGTTGGGAATGGAACTTGGACTCCAT GCATGCTAGTAGGCACACTTCTGACTGTACTCCAGCCCCAGCCTGAGTGGAGCCTTCTCATCCACTGCCT ACGCAGGCCAGCCTGCTATCTGTACCTCTTTAAGAAAGAGACTGTGAGCCTCAAAGGTTCAGTGAGCCAG ATAAATGGCCTTTCCATGACTGGTCTCTGGCAAGAAAACCATGCTTTAGGCTGGAACAATTTAAAGTGTT TATTACATGCTACTGGAGTATATTGGCTTTTAATGTCCACATATAAGATGACATACAGGCAATTTAAAAT AACATACACAGTACTGACATATTTATACTTAGAACTTACACAAATATTTACAAACATACAAATATAGGAC AATGTTTACAAAAACTTATTAATTACATGTTTATAACACTTCAGAAATAAAATCTGCTGTAAACCATCTC ACTGCAATGAAAAAGGCTTCTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310844 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310844 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17365 same embryo
	EMAGE:17367 same embryo
	EMAGE:17366 same embryo
	EurExpress:euxassay_003706 same experiment
	MGI:4825611 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source