EMAGE mouse gene expression spatial database

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EMAGE:17365

Ttc29 tetratricopeptide repeat domain 29 ( MGI:1920551)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003693. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003693_01	euxassay_003693_02	euxassay_003693_03	euxassay_003693_04

euxassay_003693_05	euxassay_003693_06	euxassay_003693_07	euxassay_003693_08	euxassay_003693_09

euxassay_003693_10	euxassay_003693_11	euxassay_003693_12	euxassay_003693_13	euxassay_003693_14

euxassay_003693_15	euxassay_003693_16	euxassay_003693_17	euxassay_003693_18	euxassay_003693_19

euxassay_003693_20	euxassay_003693_21	euxassay_003693_22	euxassay_003693_23	euxassay_003693_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17365_wholemount_strong.wlz

17365_wholemount_moderate.wlz

17365_wholemount_weak.wlz

17365_wholemount_possible.wlz

17365_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17365_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 18 19 moderate expression: see section 08 09 10 11 12 13 15 16 17 weak expression: see section 05 14

4th ventricle lateral recess

strong

regional

strong expression: see section 02 03 18 19 moderate expression: see section 08 09 11 12 13 15 16 17 20 weak expression: see section 04 05 06 07 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7739
Entity Detected:	Ttc29, tetratricopeptide repeat domain 29 ( MGI:1920551)
Sequence:	sense strand is shown >T7739 GCCAGGCCAACAATCTGGGATGAGAGTACCGCGCCCAGCACCCACCGCCTGCAGAAGCTTAGTCAGGGAC ACAGAATCACAGGCCCAGAGCTAGTTTTCTAAGGTTTGTGGGGCAAGGCTGGGGACAGGACCTGGTCAGC TCCCAGAAGAGTGGGCGGCTCTTCCTTTCCAAGAACCCCTTCTTCCATTACTTGGGCCCCAGCTTGGTAT TTTGCTTACAGAATTTTCAGACAGGCCTCTAAGAGCCCAGAGCAATCGGTTATCTGCAAATGTGGATGGC TCTGTATTCACATACTTTGCATAAGGACAGACTTTTTACAACAGGACTGTGTGGCCTGGAGGCCCTGACC TAAGAGGGGCTCAACTTCTGGACATTTCATTAAGCCCCCCAATGGCTACCTTTCCCCCACTTCCCATGAC TCATACAAGGCTTGCGATCTTAGCCAGGCAGAAGCTACCATGCTCTTCAAAAAAAATCCCAAGGGCCCAG TTGATCAAAGAGAAAGAGGACATAGATTATTACCTGGAACAGAATTTCAAAGGGCTGTCGAAAGAAGAGG TGGCTGCCCACAGGAACTCATACAAGAAGAGCATCTGCGTGGACATGCTGCGAGATGGGTTCCACAAGTC CTTCACCGAGCTCTTTGCACTGATGGAACAGTGGGATAAGCTCCCGGAAGCTGCGAAGGCACAGTCCCTC TTCTGGCAGCAGAGGCCTCTGGAGGACCAGCCGGACAAGCTAGACAATTTCTACCACTACCTGACCAGGG CCGAGGCTGCCGAGAGGAAAGGATACTATGAGGAGGTATATAACAACCTGTATGCTCTGGCCTGTTATTT CGATAATTCTGAAGACAAGTGGGTGAGGAATCACTTCTACGAACGGTGTTTTAATATTGCTCAGCTGATC AAAGCTGACGGCGGGAAGAAAGAGGCAGAGGCCGAGTCACACATGGGACTTCTTTTCGAGGAAGAGGGTG AGCTCCTGAAGGCTGCAGAGCACTATGAAGCATTCCATGAACTGACACACGGACGGCTCTGGAAGGATGG TACGGGCCAGCTTCTCAACTTGGTGGCCTGTGAAAGTCTCGTGAGGACCTACAGATTGCTCTCAGACAGG ATGCTGGAAAACAAAGACTACAAACAGGCCATCAAAATCCTAATAAAAGCTTCTGAAATAGCCAGAGAAG GCAATGACAGAAGCATGGAAGGGGAGGCTTCTTACTACCTGGGCTTGGCCCACTTGGCTTCTGGAGAATA TGAAACCGCACTAACAGTGCTGAATCGGTACAGTGAGATCTCCACAAGTCTGGATGACGATCATGGCCTG GGGAGAGCCTATGAGGCTATCGCCAAGGCCCTGCAGAGCCAAGGGGAGACAACAGAAGCAATTAATTACC TGGAGAAGTTCGTGACAATTGCAAGAAACAACTTGCAAAGCCTCGATATGATTCGAGCATGCACGATGCT CGGCGACATCTACAATGAAAAGGGGCAATACAGCAAGGCTTCCGAGTACTTCCAACAAGCTTTCAGCACC GCGATGGAGCTCATGAAAACGGCGCTCATGGATGAAACCAAAGTCCACTATGGGATAGCCAGAGCGCACC AGATGATGCTGGCGATGAAGGGTTACATCGAATCTGCCGACTCGAATGGCCTCAACTGCCTGCTGTCCTG GAAGGAGACTAGAACTCAAATCGAGTACGACCCGATTCTAGGAGAGTCTAGAAGAGCCACAGAAGATAAC ATATATCAACTCCCAGATGCTGAGGAAGAAACAAGAAGGTCTCCAGAAAATCAATAACACGGAACTGAAG TGGCTCAACGTCAAAGCAGGAAGAAACTTAGCACATAGCCTTAGACCTCATTAGACAGGAGCACGTAACA GCTGATAGGGAGTTGTAAAAAGAAGATTACAGTTGTCTTTATTGAATTTTGTGCTGCCGTAACTATGGCA AGCTTATGAAGATGAAGTACAGCTTAGATGGTTAGAACGATCAGCACTTACTCATAAGATAAACTTTAGA ATGTCCCCTCCTACATGCTACTCCACAAGAACTCCAGGAGTTTAAATCTGAACTGTTTCTGATTGCAAAA TGTTTACGAGTTAAAGACATCTTTCACACTGCATGATAATCTGCTAAAATCTCAGGGAAACCTCAGCAGA CACAAAGGGCCATGAGCAGTCTGTGCTTGTATTGCTAAGCACTGCTAAATTTGCTTTATGTGTAGAAACT TAAATTCATGGCACGCTTAGCTGACAGGACACAGCCTAACATACCCACGTGCCTCGAGGCAGGGGGGAAA TCCTTCAGTCTCAGGAACCTTTGCTGGTTTGCTTGCAACTGAAAGACCGCAGCTTCCCTCTGAAACCTCT GGAACTCCTCCCGTGAATCCATAAGGGTAGTACATTACCAAGAATAAAGACTTTCTTCCTTTTCAATGCA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310247 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310247 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17367 same embryo
	EMAGE:17364 same embryo
	EMAGE:17366 same embryo
	EurExpress:euxassay_003693 same experiment
	MGI:4828984 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source