EMAGE mouse gene expression spatial database

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EMAGE:17367

Cnga2 cyclic nucleotide gated channel alpha 2 ( MGI:108040)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003698. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003698_01	euxassay_003698_02	euxassay_003698_03	euxassay_003698_04

euxassay_003698_05	euxassay_003698_06	euxassay_003698_07	euxassay_003698_08	euxassay_003698_09

euxassay_003698_10	euxassay_003698_11	euxassay_003698_12	euxassay_003698_13	euxassay_003698_14

euxassay_003698_15	euxassay_003698_16	euxassay_003698_17	euxassay_003698_18	euxassay_003698_19

euxassay_003698_20	euxassay_003698_21	euxassay_003698_22	euxassay_003698_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17367_wholemount_strong.wlz

17367_wholemount_moderate.wlz

17367_wholemount_weak.wlz

17367_wholemount_possible.wlz

17367_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17367_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18

heart

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

urethra of male

moderate

spotted

moderate expression: see section 10 11

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7745
Entity Detected:	Cnga2, cyclic nucleotide gated channel alpha 2 ( MGI:108040)
Sequence:	sense strand is shown >T7745 CGGACTACATTGACTAAGGGACAGGGGCACGATGTACTAGGCTCTCAAGCTCACTTAGCTGTGGTTATGT GCTCCATTGGTCCTCTGTTGGCTTCCTGGGCTGGTATCAGACAACGTAGGGTAAATCTCTGATTGGGAGG CTGCTGCTGTTTGTTCGGGTCTCAGAGAACCTTCCCTGGCTAGGCAACAGAAGAAGCAAGAAATCTCCAC AGGTTTCAGCGCTCATGAGCTCCCAAGACCTTCTACTTGATTGGAGCTGTTGTGGACAGAACAACAGATG CTGACTTTGGCCCCAGGACTCTGAAACCATCTGACTGGTGAGAGCCCTGGATGTACATGGATGATGACCG AAAAATCCAACGGTGTGAAGAGCTCTCCAGCTAATAACCATAACCATCATCCTCCTCCTTCTATCAAGGC CAATGGCAAAGATGACCACAGGGCAGGTAGCAGACCACAGTCTGTGGCAGCTGATGATGACACTTCCTCA GAACTGCAAAGGTTGGCAGAGATGGATACTCCACGCAGGGGAAGGGGTGGCTTCCGAAGGATTGTCCGCC TGGTGGGGATCATCAGAGACTGGGCCAACAAAAATTTCCGTGAGGAGGAACCAAGACCTGACTCCTTCCT AGAGCGTTTCCGTGGGCCTGAGCTCCAGACTGTGACAACCCATCAGGGGGATGGCAAAGGCGACAAGGAC GGCGAGGGAAAGGGCACCAAAAAGAAATTTGAACTGTTTGTTTTGGACCCAGCTGGAGACTGGTATTACC GTTGGTTGTTTGTCATTGCCATGCCTGTTCTTTACAACTGGTGTCTGTTGGTAGCCAGAGCCTGCTTCAG TGATCTACAGAGAAACTATTTTGTGGTATGGCTGGTGCTGGATTACTTCTCAGACACTGTTTATATTGCA GACCTCATCATTCGGCTGCGCACAGGCTTCCTAGAACAAGGGCTCCTGGTCAAAGACCCCAAGAAATTGC GAGACAACTATATCCACACTTTGCAGTTCAAATTGGATGTGGCTTCTATCATCCCCACTGACCTCATTTA TTTTGCTGTGGGTATCCACAGCCCTGAGGTACGCTTTAATCGTCTGTTACACTTTGCCCGTATGTTTGAG TTCTTTGACCGCACTGAGACACGTACAAGCTACCCCAACATCTTCCGAATCAGCAACCTGGTCCTCTACA TCTTGGTCATCATCCACTGGAATGCTTGTATTTACTATGCTATCTCTAAGTCCATTGGCTTTGGGGTTGA CACCTGGGTTTACCCCAACATTACTGACCCTGAATATGGCTACCTGGCTAGGGAGTACATTTACTGCCTT TACTGGTCCACACTGACCCTCACCACCATTGGAGAGACACCACCCCCTGTAAAGGATGAGGAGTACCTAT TTGTCATCTTTGACTTCCTGATTGGTGTCCTCATCTTTGCCACTATTGTGGGAAATGTGGGCTCCATGAT CTCCAACATGAATGCCACACGAGCAGAGTTCCAGGCCAAGATTGATGCTGTCAAACACTACATGCAGTTC CGAAAGGTCAGTAAAGACATGGAAGCCAAGGTCATCAAATGGTTTGACTACTTGTGGACCAATAAGAAGA CAGTAGATGAACGAGAAGTCCTAAAGAACCTGCCTGCAAAGCTTAGGGCAGAGATAGCCATTAATGTTCA TTTGTCCACTCTGAAGAAAGTGCGCATATTCCAGGATTGTGAAGCTGGCCTGCTGGTGGAACTGGTACTG AAGCTTCGTCCTCAGGTCTTTAGTCCTGGAGATTATATTTGCCGTAAGGGGGACATTGGCAAGGAAATGT ACATCATCAAGGAGGGCAAATTGGCAGTGGTAGCTGATGATGGTGTGACTCAGTATGCCTTGCTGTCAGC TGGGAGCTGCTTTGGTGAGATTAGTATCCTTAACATTAAGGGTAGCAAAATGGGCAATCGGCGCACTGCC AATATCCGTAGTCTGGGCTACTCAGATCTCTTCTGCTTGTCCAAGGATGATCTTATGGAAGCTGTGACTG AGTATCCTGATGCCAAGAAAGTCCTGGAAGAACGGGGTAGGGAGATCCTGATGAAGGAAGGTCTACTGGA TGAGAATGAAGTGGCAGCTAGTATGGAGGTAGATGTTCAGGAGAAGCTGGAGCAGCTGGAGACAAACATG GAGACCTTGTACACTCGTTTTGCCCGCCTGCTGGCTGAGTACACTGGAGCCCAGCAGAAGCTCAAACAGC GCATCACAGTGCTGGAGACCAAGATGAAACAGAACCATGAGGATGATTACCTATCAGATGGGATAAACAC CCCTGAGCCAGCTGTTGCTGAATAGCCATAGGTGCCTGTCCAGCCTTGCTTTGACCCCAGGAGTTAGAAG TGCTGTATAGAACTTTACATTTACACACATTATGCTCATGTCCCTCCGAACTCCCAAAGCCTTGCTGAGG CTTAAGGTTTTGGCTACATCTTGGAGTCCCCTTCCAAGTCCAGCTAACAGCCAAGCTTGTGGATAATGGA GATCATGTGGGTTGAATTTCTAAGAGCGTGACCTCCTAAGTCTCACAAGGAATCAGAGAATAGCTAAATT GTCCTTCCTGAGGCTTTTCTGGGACTAGATAACTAGACAGTGTTCTCTGAAGAACACTGTGCACCACAAT GCCTGCCTGACTCCTTTTAGCTTCTTTATATCGAGTCACTCCCTACTGCATTCTGCCCCAAATACCTTTT TAATGTGTTCTCTGAACAGCCTGTTTCCATGCACTTGTACACATTTAAGAATTGGCTGCAGACACTGGGC CCCCTACCCTGCCTCCCAAGGCATGCAAGGTCTGTGAGGGGAGTGGGAAGGTGGGTTTGAGTGTTGGTGC TCAGGGTCATACTTCCTTGTCAGACAATGTCACTCTGAGAAGGAGGTGGCTGGCAGCTTTGGCCATCACA CCTTATGCACACAAGTTCTGAAGAGTTTGTGAAGGCTACTGCTGCATGAGATACTGTGAATTAGAGGCAC TTAAAAGTTAATAAATTCCTTTCAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310696 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310696 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17365 same embryo
	EMAGE:17364 same embryo
	EMAGE:17366 same embryo
	EurExpress:euxassay_003698 same experiment
	MGI:4823953 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source