EMAGE mouse gene expression spatial database

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EMAGE:17369

Lrrc6 leucine rich repeat containing 6 (testis) ( MGI:1859553)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003708. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003708_01	euxassay_003708_02	euxassay_003708_03	euxassay_003708_04

euxassay_003708_05	euxassay_003708_06	euxassay_003708_07	euxassay_003708_08	euxassay_003708_09

euxassay_003708_10	euxassay_003708_11	euxassay_003708_12	euxassay_003708_13	euxassay_003708_14

euxassay_003708_15	euxassay_003708_16	euxassay_003708_17	euxassay_003708_18	euxassay_003708_19

euxassay_003708_20	euxassay_003708_21	euxassay_003708_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17369_wholemount_strong.wlz

17369_wholemount_moderate.wlz

17369_wholemount_weak.wlz

17369_wholemount_possible.wlz

17369_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17369_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7741
Entity Detected:	Lrrc6, leucine rich repeat containing 6 (testis) ( MGI:1859553)
Sequence:	sense strand is shown >T7741 GGCGCAGCTGCTAGGGCTGAGGGAAATCCTGCCTGCCGGTCTCCCTGTTTCCCTGACCAGAGATCCAGAA AAGCACCATGGGCCGAATCACAGAAGATCTTATTAGACGGAATGCTGAGCACAATGACTGTGTAATTTTT TCCCTGGAGGAACTCTCCTTGCATCAGCAAGAAATAGAAAGGCTAGAACATATTGACAAATGGTGCCGGG ACTTAAAAATTCTCTATCTTCAAAATAACCTCATTGGAAAAATTGAAAATGTTAGCAAACTGAAGAAACT TGAATATTTGAACTTAGCATTGAACAACATTGAAAGAATTGAAAACTTGGAAGGATGTGAGTGGCTGACA AAACTTGACTTGACTGTAAATTTCATTGGAGAACTGAGCAGCGTTAAAACCCTGACACACAATATTCATC TGAAGGAGTTGTTCCTCATGGGTAACCCATGTGCTGACTTTGATGGCTACAGGCAGTTTGTGGTGGTGAC CTTACAACAGTTAAAGTGGTTGGATGGCAAAGAAATAGAGCGTTCTGAAAGGATCCAGGCATTGCAGAAC TACACATCAGTTGAGCAGCAAATCAGAGAGCAGGAGAAAGCTTACTGCCTTAGACGAGCCAAAGAGAAAG AGGAAGCTCAGAGGAAACTCGAGGAAGAAAATGAAAGTGAAGACAAGAAGAAGAGCAGCACAGGCTTTGA TGGGCATTGGTACACAGACATCCACACTGCTTGCCCATCTGCCACAGAAAACCAAGACTACCCCCAGGTA CCAGAAACTCAAGAAGAGCAACACAACACAAAGGAATCAGATGACATTGAAGATGATCTGGCATTCTGGA ACAAACCGTCTTTGTTCACCCCTGAATCTAGACTGGAAACTCTCAGACACATGGAAAAACAGCGAAAAGC CCAAGATAAGTTAAGTGAAAAGAAAAAGAAAGCGAAACCACCCCGCACTTTGATTACAGAAGATGGGAAA GTCCTGAATGTCAACGAAGCCAAACTTGATTTCTCTTTGAAAGATGATGAGAAACACAATCAGATCATCC TGGACCTGGCTGTCTATAGGTACATGGATACCTCTTTGATTGAGGTTGATGTGCAGCCAACTTACGTTCG AGTAATGGTCAAAGGAAAGCCATTTCAACTTGCCCTTTCTACAGAGGTCCAGCCTGACCGCAGCTCTGCT AAAAGGTCCCAGACAACAGGACATTTGCTGATCTGCATGCCCAAGGTAGGAGAAATGATCACAGGAGGTC AACGAACACCCACGTCTGTGAAAACCACCTCAACTAGTAGCAGGGAACAAACAAACCCCAGAAAAAAGCA GATAGAAAGATTGGAAGTTGACCCCAGCAAGCATTCTTGCCCTGATGTGTCTACCATAGTACAAGAGAAA AGACACAGACCCAAAAGAATGGAATCTCAACCCCGAGATGAACCCAGTGAGGAAGATCCAGACTTTGAGG ATAACCCAGAAGTGCCTCCACTAATTTGAAATAGGAGGCTGTGTTGTCATTGACCCTGACCCACAGACTC ATGTTGCTTGGTGTTGACAGTGGCTATGTGTCATTCATGGAGTAAACATAGTTCATTATCATTTTTACCT GATGCTATAATGCTTTCCATGCATTGTAATATATTTGAGTTTACAATTAAATTGCATACCTTAAATATGC TATAACTTATCACTCATCTATAATTGAACTTCGAGCTTGAAAATGTGAAGTAATTTAATAGAACTACGAC ATTCAACATTAAGAATAAGTCATTTAGTCTTCACTTATGCAATAGATTGTGTTATATGGAAAAGGTCTTT AGCAAATTGTTCAAACTCAGAAGTTAAAACTGTACTCTCAGGTCGAACGAAAATGCCCTGACATGATTTT AAATAGTATTTTTTTATTTTTCTTCTCACGTTTCTGCTTTCTCTAGTGGCATGATGTAATGGCAAGAATA AGAACTTTGACATTAGATTTCCTGTATTTAGATTCCTGCTGAGTCACTGAATAAAGACTTTCAGCTGGTC CTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6310345 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6310345 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17370 same embryo
	EMAGE:17368 same embryo
	EMAGE:17371 same embryo
	EMAGE:17372 same embryo
	EMAGE:17373 same embryo
	EurExpress:euxassay_003708 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source