EMAGE mouse gene expression spatial database

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EMAGE:17381

Ebf2 early B-cell factor 2 ( MGI:894332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003814. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003814_01	euxassay_003814_02	euxassay_003814_03	euxassay_003814_04

euxassay_003814_05	euxassay_003814_06	euxassay_003814_07	euxassay_003814_08	euxassay_003814_09

euxassay_003814_10	euxassay_003814_11	euxassay_003814_12	euxassay_003814_13	euxassay_003814_14

euxassay_003814_15	euxassay_003814_16	euxassay_003814_17	euxassay_003814_18	euxassay_003814_19

euxassay_003814_20	euxassay_003814_21	euxassay_003814_22	euxassay_003814_23	euxassay_003814_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17381_wholemount_strong.wlz

17381_wholemount_moderate.wlz

17381_wholemount_weak.wlz

17381_wholemount_possible.wlz

17381_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17381_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7755
Entity Detected:	Ebf2, early B-cell factor 2 ( MGI:894332)
Sequence:	sense strand is shown >T7755 GTTCTGCAGCTGGAAGGATGCGGGCGACCTAGAGTGGAAGCCCCGAGGCGGCAGATCTGAAGCCACCGAG GATAAAAAACGCAAACTTTGCATTGTGCGTGGAGGAACGGGCTGGCTATTTCTTTCCAAGAGGGCCCCCA AACTTCGGCCCTTGCCGCCTTTCTCGGATTGCTTTTGGAGAACACCATTTGCTGGCAGCATTGGAGAGTG AGGACGGTGTTGTTGTAAAGTCACTTTTAAGGCGACAGTAGAGGACCAGACTTTTTAAATGTTTGGGATT CAAGATACGCTAGGAAGAGGACCAGCTCTGAAAGACAAGTCGCTGGGTGCCGAGATGGATTCGGTCAGGT CCTGGGTCCGGAATGTCGGCGTGGTGGACGCCAATGTCGCGGCTCAGAGCGGAGTTGCTCTGTCCCGGGC CCATTTTGAAAAGCAACCTCCTTCAAACTTGAGGAAATCCAACTTCTTTCATTTTGTCCTGGCGCTCTAC GACAGGCAGGGACAGCCGGTGGAGATTGAGCGCACAGCCTTCGTGGACTTTGTGGAGAATGACAAAGAGC AAGGCAATGAGAAGACCAACAATGGTACTCACTACAAGTTACAGCTCCTTTACAGCAACGGTGTCCGCAC GGAGCAGGACCTGTATGTCAGGCTCATCGACTCTGTCACCAAACAGCCCATAGCCTATGAGGGACAGAAT AAGAATCCCGAAATGTGCAGAGTTCTGCTGACACACGAGGTGATGTGCAGTCGATGCTGTGAGAAGAAGA GCTGCGGGAACCGGAACGAGACCCCTTCAGACCCGGTCATCATCGACAGATTCTTTTTAAAATTTTTCCT GAAGTGCAATCAGAATTGTTTGAAAACAGCGGGAAACCCAAGGGACATGAGGCGGTTCCAGGTCGTGTTG TCCACAACAGTAAATGTGGATGGACATGTGCTGGCTGTTTCTGACAACATGTTTGTTCATAACAATTCTA AGCATGGAAGGAGAGCAAGAAGGCTTGACCCATCTGAAGCTACACCCTGCATCAAAGCCATCAGCCCAAG TGAAGGCTGGACCACAGGAGGGGCCATGGTCATCATCATTGGTGACAACTTTTTTGATGGTCTCCAGGTT GTGTTTGGTACCATGCTTGTATGGAGTGAGCTCATAACCCCTCATGCCATCAGAGTACAGACCCCTCCCC GGCATATCCCTGGAGTTGTGGAAGTGACACTATCTTATAAATCCAAACAGTTCTGCAAAGGAGCCCCGGG CAGGTTCATCTACACAGCCTTAAATGAACCCACCATAGACTATGGCTTCCAAAGATTGCAGAAAGTCATC CCAAGGCACCCAGGTGACCCTGAGAGGCTAGCTAAGGAAATGCTGTTGAAAAGAGCTGCGGACCTGGTGG AAGCCCTCTACGGCACACCACACAATAATCAGGACATCATTTTAAAGCGTGCTGCAGACATTGCTGAAGC CCTGTACAGTGTCCCCAGGAACCCCAGCCAGATTCCAGCTCTTTCCAGCTCCCCAGCTCACAGCGGTATG ATGGGTATCAACTCTTACGGCAGCCAGCTCGGCGTCAGCATTTCAGAGTCCACACAAGGAAATAACCAAG GATACATCCGCAACACAAGCAGCATCTCCCCGCGGGGATACTCCTCCAGCTCCACGCCTCAGCAATCTAA TTACAGCACCTCCAGCAACAGCATGAACGGCTACAGCAATGTTCCTATGGCCAACCTGGGTGTGCCAGGA TCCCCGGGATTTCTGAATGGCTCACCCACGGGCTCCCCATATGGAATCATGTCTTCAAGCCCCACCGTGG GATCCTCCAGCACGTCCTCCATCCTCCCGTTCTCCTCTTCAGTTTTTCCTGCTGTCAAACAGAAGAGTGC CTTTGCCCCTGTCATCAGGCCCCAAGGCTCCCCATCGCCTGCCTGCTCCAGCGGCAATGGGAACGGATTC AGAGCCATGACAGGACTTGTTGTTCCCCCGATGTAAAGCGGAAGAAGAACTGCTTTCTCATAGCACAAAA CTACTTATTCCGATGGACCAATTATGAAGGAAGCACTCTGAGCTTGTTGGGGAGAGTTGCGCCCCCAAGC GGCCATGTTGGACAGATTTGGGAAGGCAGAGAGCCGCCATCTTGGTCTCGTGTTCCTCATGAAGCATTGA CAGTCACTACACAAACTGACCCTTTTGGAGACAAGAGCAGAGAAATGTCATTGACGTGGTCAGTGCTAAG AGCAGCAATTCTTTGTTATGAACATTACAAAAACCACCTTCCTATGTTTGTAAAATATTTAAGAAAAACA TTGGCAAACATTCATGCTTAATATTTTGGATACTATTTGTTTTTCTTTGTAGGAAAAAAAAGTTGAAAGT TTCTATTTTCTATGAATCCTTTCAGATACCAATTTAGTTTATGCAGAAAAAAATTGAACAAAACAGGGTA CCAGCATGGAAGACTTTCTTAAAATGAAACCTGAATTGAATGAGGAAATGTTGTATGTGTGTTTGCTTAT ACTTTAAGCTCTTTTAAAAAAAAAGGGAAAAAAATTTAAAATGTTTTACAGTTATTTAAATAAATAAACG TGTAACTTATTGTAAGTAGAGGTAGAAATTAAGACCTTTTTTAAGAAGAGAAGAATTTCTCTTCCTGATG TAAAATGAAATTGACACACGTAGTGACCAGTTTTAAAGGTGTGTTGTTATTACAGTTGCTTATTAGATTC TTATCCTACATCCCACACCCCCCTCTTTTTCCATCACTTTATCAACCCATGCACAAGAATGTGTAACTTG TGCAGATTCCCACAGAATCCACCACAGAAACCAGACTCAGATAAACGCCAGCACACCAACACTTCAATCC ACCACTCCATTCCCAGATCTGCTTTCTAGTTCACAGATCCCTTATGGTGTCCAATCTTGGTGAAGCGTTT TGAAAAAATACATTCCTCCCTGAGCAATGCAGGGAGTCCCCATGTTACCCTCTCAGCTTTGAACATCCTC AGGCAAGAAGTATCTCACCTCTAAGGAGAGTCAAAGCCCCATGGCTACACTGACCAAGGACTCTTCAGAA TTTCTCTCCAACAGAGCCCCACAGTCCTTCCTCTTTGGGTACTCGATTCAGGCATGGAGGCAGGAAGCTC CATAGAACTTTGGAGAAAGAACTTGGGGAGCTTGGTTGTGTTTCTTGGACTCTTAAGACTCAGTCTTTGA CTGTTTCCTTTCTATGCAGAGTTTTTCTTTTATGTTAGTAAACTCCCTTGGAAAAGCATGTATCTCACCT GACACTGTTTCTGAGATGAGCAGGGTGGGGTGAAGAGGAACGAACTCAAAACCCCCTAAGTGGCTCTCTT ATTGGGAGATTCGTGGCAGCTGTGTTCCAAGCGGTGCAGTTACCCAGCCTGCCCTGGGAGCCTTGTGCTA CCTACCTAGTTCTTAGCAGGGAAAACCTTGCAGTAGTTGCTAACAGTGGTAGACTCTTGAAAATGTGTGT GGTCCTGGAGATGCATCTAGACTTGCTTGGCTTGCCAATAGGATGTCATGATTTTCCAGGTGTCTTCTCT TTGATTCCAATGACTTAGCTTGGGGTTCTTGGTTAGGCTTCTAGCATTGGAAATTGATGTGTCTTTCACG GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6312260 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6312260 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17383 same embryo
	EMAGE:17382 same embryo
	EMAGE:17380 same embryo
	EMAGE:17378 same embryo
	EMAGE:17379 same embryo
	EurExpress:euxassay_003814 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source