EMAGE mouse gene expression spatial database

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EMAGE:17399

Emid2 EMI domain containing 2 ( MGI:2155345)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003830. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003830_01	euxassay_003830_02	euxassay_003830_03	euxassay_003830_04

euxassay_003830_05	euxassay_003830_06	euxassay_003830_07	euxassay_003830_08	euxassay_003830_09

euxassay_003830_10	euxassay_003830_11	euxassay_003830_12	euxassay_003830_13	euxassay_003830_14

euxassay_003830_15	euxassay_003830_16	euxassay_003830_17	euxassay_003830_18	euxassay_003830_19

euxassay_003830_20	euxassay_003830_21	euxassay_003830_22	euxassay_003830_23	euxassay_003830_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:17399

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17399_wholemount_strong.wlz

17399_wholemount_moderate.wlz

17399_wholemount_weak.wlz

17399_wholemount_possible.wlz

17399_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17399_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

moderate

regional

moderate expression: see section 08 09 10 11 12 13 16 22 23 24

mesenchyme

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

skeleton

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

moderate

regional

moderate expression: see section 14 15 16 17 18 19 not examined expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7775
Entity Detected:	Emid2, EMI domain containing 2 ( MGI:2155345)
Sequence:	sense strand is shown >T7775 GGCTAGCTCCCTGCGCTCCAGCCTGGGGCAACAGGGTCAGTCCAGCGGTCTGGGGCACTCCTGATCGGCT CCCGGCTCCCGGGACCCTAGGTCCCCGCGGGAGAGCGGTGCAAAATGAAGCTGGTTTTGCTGTTACCATG GGCGTGCTGCTGCTTGTGCGGGTCGGCGCTGGCCACCGGCTTTCTCTACCCGTTCCCGGCAGCAGCCTTG CAGCAGCACGGCTACCCGGAGCAGGGCGCCGGCTCCCCCGGCAACGGCTACTCGAGTCGCCGGCACTGGT GCCATCACACGGTGACAAGGACAGTGTCCTGCCAGGTGCAGAATGGCTCCGAGACGGTGGTCCAGCGAGT CTACCAGAGCTGCCGGTGGCCTGGGCCCTGTGCCAACCTCGTGAGTTACAGGACTCTCATTAGACCGACC TACCGGGTTTCCTACCGCACGGTGACTGCGCTGGAGTGGAGGTGTTGCCCAGGATTCACCGGAAGCAACT GCGAGGAAGAATGTATGAACTGTACCCGGCTTAGCGACATGAGTGAACGGCTGACCACGCTGGAGGCCAA GGTCCTCTTGCTGGAAGCAGCTGAGCAGCCTTCAGGTCCAGACAATGACCTGCCACCCCCGCAGAGCACC CCACCAACCTGGAACGAGGACTTCCTGCCAGACGCCATTCCCATCGCACATCCAGGGCCCCGGAGGAGAA GACCCACAGGTCCAGCTGGACCTCCAGGACAGATGGGACCACCTGGACCTGCAGGACCTCCAGGTTCCAA AGGTGAACAGGGCCAGACAGGAGAGAAGGGCCCAGTGGGACCTCCAGGTCTCTTGGGACCACCGGGGCCA CGCGGGCTCCCTGGAGAGATGGGGCGCCCTGGTCCACCAGGCCCTCCTGGCCCAGCAGGAAGTCCTGGCC TCTTGCCAAACACTCCCCAAGGCGTCCTGTATTCCCTGCAGACACCAACAGACAAGGAGAATGGAGACTC CCAGCTGAACCCTGCTGTTGTAGACACAGTGCTGACTGGCATCCCAGGTCCCCGGGGTCCCCCAGGCCCC CCTGGTCCCCCAGGACCACACGGCCCCCCAGGACCCCCAGGAGCACCTGGATCCCAGGGCCTGGTGGATG AGCGGGTTGTGGCAAGGCCATCTGGTGAGCCCAGTGTGAAGGAAGAAGAGGACAAAGCCAGCGCTGCAGA GGGAGAAGGTGTGCAGCAGCTTCGCGAGGCCCTGAAGATCCTGGCAGAGAGGGTCCTTATCCTTGAGCAT ATGATTGGAGTCCATGATCCCCTGGCCTCTCCTGAGGGTGGCTCTGGGCAGGATGCAGCCCTGAGAGCCA ATCTCAAGATGAAGCGAGGAGGTCCTCGACCTGACGGCATCCTGGCTGCCCTGCTGGGCCCCGACCCTGC ACAAAAGAGCGCAGACCAGGCTGGCGACAGGAAGTAAGAGTCCCTCTGTCCAGTGCGAGCCCAGTGTTAC AGCTGAACAGCGGGTGCATCCTGAAGACTCCCCATGTGGGACTGGGCATCAAGCAGGGACCATTGTGGAG GACATTGGGAGCCTGGGGTCCCTGGGGAGAGATGGTGCTTCCAAGGGCAGGGCCCAGTGCTGACTAGCAC CCCGACAGAGCTCTTCTTTTGCCCCTGCCCCCTACCTCCCACTCCCGCTGGAGACGGGGTCTGGGTAAGC TGGGTGAGCTGGGTGGGCTAGGTGGAGGTGGGAGAATAATCACAACCCTCAATGGCTGAGCCCCTGCTCT GTCAGGGCCAGGGCCAGCTCAGGCACTTTCCCTATATTGTCTCATTTAATCCTTAAAAGGTAGGTCTGTG CTCCCCACTCAGCCTGGGGACCGATGTGCACATGGTACACATGACCCGGATTCTGGATGTGGGGGCCTGG GGGGTAGGTTTTAACCCAGACCTCACTGTTGTTGAAGCCATGACCCTGAGGTGTGTGGCCCAGAGGATTC CTGAGGCAGCTGGGCTGCCGGGAGGCAGGGCCACCTGGAAAGACATCAGGGCACTACAAAATTTTGGGCC TGCCTCTCTTCCTCTGGTCTTCCTTAGGGCTGCGGGTAGGGGTCAGCTTCCAAGAGGGGAGTTTGGGACT GTGAGGGAGGAGGGTCTCTGAGAATGAGAAAGGCGAAGAGCTTTGGCGATGCCCCCTAATGTCTCCCAGT CTTGCTGAGCCTACTGGTCCTTATCTGCCCTCCCCCATGGAGAAGGAAGGACCATTTAATGAGACCATCC ATCCAGAATGGGACCTACAACCTCCATCATGGCCACTCTTTCATGAATATCTATATCCTAATTTGATGGA CAACACAAATCCGTGTCCACTTAACAGAGTCAGACTGCCTGTATTCTAGGCTTGCTAGGGGCCGTGGCCA CACACCTTGAGGCTGGGGTCCAGGCAGGTTACACAGAAGAACTGCCCCACAGCCCTGAAGGCTTCTCTGG CTGGTGCTTGGTCTCTTATGACGCCCTCCCCCCATCCTGGTGCTCCTATAACTGGGCCAATGGCCCTGAC ACTCAGAGAACAGAAAACACCACCCTCGGGTATGTCTCCCTGCCCAGCAGGAGTGACCATCCCTAAAGTT CTTGGTGCTTATGCAAGGCTGTGACCTGACCCCACTGGGCCCTGTAGGCTCTCTCTGAGTTATTCTCTGT CTCCACCAGGAGGTGATCAGAGACCTGTGATGTTATTTCAAATAAAGGTGCTCTCTCCACTGTGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6313353 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6313353 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17394 same embryo
	EMAGE:17398 same embryo
	EMAGE:17397 same embryo
	EMAGE:17396 same embryo
	EMAGE:17395 same embryo
	EurExpress:euxassay_003830 same experiment
	MGI:4824541 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source