EMAGE mouse gene expression spatial database

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EMAGE:17435

Gpnmb glycoprotein (transmembrane) nmb ( MGI:1934765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003884. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003884_01	euxassay_003884_02	euxassay_003884_03	euxassay_003884_04

euxassay_003884_05	euxassay_003884_06	euxassay_003884_07	euxassay_003884_08	euxassay_003884_09

euxassay_003884_10	euxassay_003884_11	euxassay_003884_12	euxassay_003884_13	euxassay_003884_14

euxassay_003884_15	euxassay_003884_16	euxassay_003884_17	euxassay_003884_18	euxassay_003884_19

euxassay_003884_20	euxassay_003884_21	euxassay_003884_22	euxassay_003884_23	euxassay_003884_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17435_wholemount_strong.wlz

17435_wholemount_moderate.wlz

17435_wholemount_weak.wlz

17435_wholemount_possible.wlz

17435_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17435_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 17 18 19 20 weak expression: see section 10

thyroid gland

weak

regional

weak expression: see section 11 15

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 20 21 22 23

heart ventricle

strong

single cell

strong expression: see section 08 09 10 11 12 13 moderate expression: see section 14 15 16 17 18

trachea

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7259
Entity Detected:	Gpnmb, glycoprotein (transmembrane) nmb ( MGI:1934765)
Sequence:	sense strand is shown >T7259 GCCGACACTGTGACTCCTGGTGGATGGGACTGGGGAGTCAGAGTCAAGCCCTGACTGGTTGCAGGCGCTC GGAGTCAGCATGGAAAGTCTCTGCGGGGTCCTGGGATTTCTGCTGCTGGCTGCAGGACTGCCTCTCCAGG CTGCCAAGCGATTTCGTGATGTGCTGGGCCATGAACAGTATCCCAATCACATGAGAGAGCACAACCAATT ACGTGGCTGGTCTTCGGATGAAAATGAATGGGATGAACACCTGTATCCAGTGTGGAGGAGGGGAGACGGC AGGTGGAAGGACTCCTGGGAAGGAGGCCGTGTGCAGGCAGTCCTGACCAGTGACTCACCGGCTCTGGTGG GTTCCAATATCACCTTTGTGGTGAACCTGGTGTTCCCCAGATGCCAGAAGGAAGATGCTAATGGCAATAT CGTCTATGAGAAGAACTGCAGGAATGATTTGGGACTGACCTCTGACCTGCATGTCTACAACTGGACTGCA GGGGCAGATGATGGTGACTGGGAAGATGGCACCAGCCGAAGCCAGCATCTCAGGTTCCCGGACAGGAGGC CCTTCCCTCGCCCCCATGGATGGAAGAAATGGAGCTTTGTCTACGTCTTTCACACACTTGGCCAGTATTT CCAAAAACTGGGTCGGTGTTCAGCACGGGTTTCTATAAACACAGTCAACTTGACAGCTGGCCCTCAGGTC ATGGAAGTGACTGTCTTTCGAAGATACGGCCGGGCATACATTCCCATCTCGAAGGTGAAAGATGTGTATG TGATAACAGATCAGATCCCTGTATTCGTGACCATGTCCCAGAAGAATGACAGGAACTTGTCTGATGAGAT CTTCCTCAGAGACCTCCCCATCGTCTTCGATGTCCTCATTCATGATCCCAGCCACTTCCTCAACGACTCT GCCATTTCCTACAAGTGGAACTTTGGGGACAACACTGGCCTGTTTGTCTCCAACAATCACACTTTGAATC ACACTTATGTGCTCAATGGAACCTTCAACCTTAACCTCACCGTGCAAACTGCAGTGCCCGGGCCATGCCC TCCCCCTTCGCCTTCGACTCCGCCTCCACCTTCAACTCCGCCCTCACCTCCGCCCTCACCTCTGCCCACA TTATCAACACCTAGCCCCTCTTTAATGCCTACTGGTTACAAATCCATGGAGCTGAGTGACATTTCCAATG AAAACTGCCGAATAAACAGATATGGCTACTTCAGAGCCACCATCACAATTGTAGAGGGGATCCTGGAAGT CAGCATCATGCAGATAGCAGATGTCCCCATGCCCACACCGCAGCCTGCCAACTCCCTGATGGACTTCACT GTGACCTGCAAAGGGGCCACCCCCATGGAAGCCTGTACGATCATCTCCGACCCCACCTGCCAGATCGCCC AGAACCGGGTCTGCAGCCCTGTGGCTGTGGATGGGCTGTGCCTGCTGTCTGTGAGAAGAGCCTTCAATGG GTCTGGCACCTACTGTGTGAATTTCACTCTGGGAGATGATGCAAGCCTGGCCCTCACCAGCACCCTGATC TCTATCCCTGGCAAAGACCCAGACTCCCCTCTGAGAGCAGTGAATGGTGTCCTGATCTCCATTGGCTGCC TGGCTGTGCTTGTCACCATGGTTACCATCTTGCTGTACAAAAAACACAAGGCGTACAAGCCAATAGGAAA CTGCCCCAGGAACACGGTCAAGGGCAAAGGCCTGAGTGTTCTCCTCAGCCACGCGAAAGCCCCGTTCTTC CGAGGAGACCAGGAGAAGGATCCATTGCTCCAGGACAAGCCAAGGACACTCTAAGTCTTTGGCCTTCCCT CTGACCAGGAACCCACTCTTCTGTGCATGTATGTGAGCTGTGCAGAAGTATGTGGCTGGGAACTGTTGTT CTCTAAGGATTATTGTAAAATGTATATCGTGGCTTAGGGAGTGTGGTTAAATAGCATTTTAGAGAAGACA TGGGAAGACTTAGTGTTTCTTCCCATCTGTATTGTGGTTTTTACACTGTTCGTGGGGTGGACACGCTGTG TCTGAAGGGGAGGTGGGGTCACTGCTACTTAAGGTCCTAGGTTAACTGGGGGAGATACCACAGATGCCTC AGCTTTCCACATAACATGGGCATGAACCCAGCTAATCACCACCTGAAGGCCATGCTTCATCTGCCTTCCA ACTCACTGAGCATGCCTGAGCTCCTGACAAAATTATAATGGGCCCGGGCTTTGTGTATGGTGCGTGTGTG TACATATTCTACTCATTAAAAAGGTAGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4164706 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4164706 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4825168 same experiment
	EurExpress:euxassay_003884 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source