EMAGE mouse gene expression spatial database

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EMAGE:17442

Oscp1 organic solute carrier partner 1 ( MGI:1916308)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003874. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003874_01	euxassay_003874_02	euxassay_003874_03	euxassay_003874_04

euxassay_003874_05	euxassay_003874_06	euxassay_003874_07	euxassay_003874_08	euxassay_003874_09

euxassay_003874_10	euxassay_003874_11	euxassay_003874_12	euxassay_003874_13	euxassay_003874_14

euxassay_003874_15	euxassay_003874_16	euxassay_003874_17	euxassay_003874_18	euxassay_003874_19

euxassay_003874_20	euxassay_003874_21	euxassay_003874_22	euxassay_003874_23	euxassay_003874_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17442_wholemount_strong.wlz

17442_wholemount_moderate.wlz

17442_wholemount_weak.wlz

17442_wholemount_possible.wlz

17442_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17442_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 13 14 15 16 17 18 19

4th ventricle lateral recess

moderate

homogeneous

moderate expression: see section 02 03 09 10 13 14 15 16 17 18 19 weak expression: see section 04 05 06 07 12 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 18

heart ventricle

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7736
Entity Detected:	Oscp1, organic solute carrier partner 1 ( MGI:1916308)
Sequence:	sense strand is shown >T7736 CAGGCTGCTAAATCACAAACCCTGGGGTTCTTCCTTCATCTACCCTGCGGGTTTCCGGCACTATGTCGGT TCGGACTCTGCCGCTGCTCTTTTTGAACTTGGGCGGGGAGATGCTTTACGTCCTAGACCAGCGACTGAGG GCCCAGAACATCCCCGGAGACAAGGCCCGCAAAGTTCTGAACGACATCATTTCAACCATGTTCAACAGGA AGTTCATGGACGAACTGTTCAAACCCCAGGAGCTGTACTCCAAGAAGGCCTTGCGGACTGTGTATGACCG CCTGGCTCATGCCTCCATCATGCGACTGAACCAGGCCAGCATGGATAAGCTCTACGACCTGATGACCATG GCTTTCAAATATCAAGTGCTGCTGTGTCCACGCCCCAAGGACGTGTTGCTGGTCACTTTCAACCACTTAG ACGCCATCAAGGGTTTTGTCCAAGACTCTCCCACCGTCATACATCAGGTGGACGAGACCTTCCGGCAGCT GTCAGAAGTGTATGGGAAGCTCTCTGAAGGGGAGTTCCAGCTGATCCGGCAGACACTGCTCAACTTCTTC CAAGACCTGCATATCCGAGTGTCCACATTTCTAAAGGACAAAGTTCAGAACTCTAATGGTCGCTTTGTGT TGCCAGTGTCGGGGCCTGTTCCCTGGGGAACTGAAGTTCCTGGAGTCATTAGAGTGTTCAGTGTCAAAGG CAAAGAGGTGAAGAAGATGAAATTCCGGCATGGCGGAGACTATGTTGCTGCCCAGAAGGAAGGTTCTTTT GAGCTTTATGGAGACCGAGTCTTGAAACTGGGAACTAACATGTACAGTGCGAGTCGTCCTGTGGAAACCC ACATGTCTGCAACATCCAAGAACGCAGCCTCCAGGGCACAGGAAAACATTGTTCCAAACCCTCTTGCCAA AGAAGAGCTGAATTTCTTGGCCAGGCTAATGGGAGGGATGGAGATCAAGAAGCCGAGTGGCCCTGAACCA GGATTCCGACTGAATCTGTTTACGACCGATGAGGAAGAGGAACACGCAGCACTGTCCAGGCCGGAGGAGT TGTCCTATGAAGTCATCAGCATACAGGCTACACAGGACCAGCAACGGAATGAGGAGTTGGCCCGGATCAT GGGTGAGTTTGAGATCACAGAGCAGCTGGAGCAGAACACCAGTAAGGGAGACGACTTGCTGGCCATGATG GACAGGTTATAGATGCAGATGGGCACGCCCTAGCCCTCCCTCAAGAGACTGCCTTAGACCACCCTGGACA CGGATGGCTGGGGATCTCATGTGCCCCTGCTGGATTCCAACCAAGGCCTGCACCTTCTAGTCCATTTTTA AGTCAGAAAAAAACTGTGACGACCTCTCGGAGAGCATCCATTTCTGAAGCCACACACATTTCTGTATTTT CAGCAAAATATATTCTTGCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309923 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309923 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17440 same embryo
	EMAGE:17439 same embryo
	EMAGE:17443 same embryo
	EMAGE:17438 same embryo
	EMAGE:17441 same embryo
	EurExpress:euxassay_003874 same experiment
	MGI:4826994 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source