EMAGE mouse gene expression spatial database

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EMAGE:17466

Tomm34 translocase of outer mitochondrial membrane 34 ( MGI:1914395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003916. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003916_01	euxassay_003916_02	euxassay_003916_03	euxassay_003916_04

euxassay_003916_05	euxassay_003916_06	euxassay_003916_07	euxassay_003916_08	euxassay_003916_09

euxassay_003916_10	euxassay_003916_11	euxassay_003916_12	euxassay_003916_13	euxassay_003916_14

euxassay_003916_15	euxassay_003916_16	euxassay_003916_17	euxassay_003916_18	euxassay_003916_19

euxassay_003916_20	euxassay_003916_21	euxassay_003916_22	euxassay_003916_23	euxassay_003916_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17466_wholemount_strong.wlz

17466_wholemount_moderate.wlz

17466_wholemount_weak.wlz

17466_wholemount_possible.wlz

17466_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17466_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 10 11 12 13

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 16 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 11 15 16 17

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7303
Entity Detected:	Tomm34, translocase of outer mitochondrial membrane 34 ( MGI:1914395)
Sequence:	sense strand is shown >T7303 AGCTGTTGCGCACCACTTAGCTGGGAAGTGCGTTGCTCCCTGTTTCCCAGCCCACCCGAGATGGCCCCCA AAGTCTCGGATTCCGTGGAACAGCTCCGCGCTGCCGGCAACCAGAACTTCCGCAATGGCCAGTACGGCGA AGCCTCGGCGCTGTACGAGCGCGCACTGCGGCTGCTGCAGGCGCGAGGTTCTGCAGACCCCGAAGAAGAA AGTGTTCTGTACTCCAACCGTGCAGCGTGCTACTTGAAGGATGGGAACTGCACAGATTGCATCAAAGATT GCACTTCCGCGCTGGCCTTGGTTCCCTTCAGCATCAAGCCCTTGCTGCGCAGAGCATCTGCATATGAAGC CCTGGAGAAGTACGCCCTGGCCTACGTTGACTATAAGACTGTGCTGCAGATCGATAACAGTGTGGCATCC GCCCTGGAAGGCATCAACAGAATAACCAGAGCTCTCATGGACTCCCTGGGACCTGAGTGGCGCCTGAAGC TGCCCCCTATCCCTGTGGTGCCTGTTTCAGCCCAGAAGAGATGGAATTCCTTGCCTTCAGATAACCACAA AGAGACAGCTAAAACCAAATCCAAAGAAGCCACAGCTACGAAGAGCAGAGTGCCTTCTGCTGGGGATGTG GAGAGAGCCAAAGCTCTGAAGGAAGAAGGCAATGACCTTGTAAAGAAGGGCAACCATAAGAAAGCTATTG AGAAGTACAGTGAGAGCCTCTTGTGTAGTAGCCTGGAGTCTGCCACATACAGCAACAGAGCGCTCTGTCA CCTGGTCCTGAAGCAGTACAAGGAGGCAGTAAAGGACTGCACAGAAGCCCTCAAGCTGGATGGGAAGAAT GTAAAGGCGTTTTACAGACGGGCTCAAGCCTACAAGGCACTCAAGGACTATAAGTCAAGCCTTTCGGATA TCAGCAGCCTCCTACAAATTGAACCCAGGAATGGCCCTGCACAGAAGTTACGGCAGGAAGTTAACCAGAA CATGAACTAAACCGTAGAGGGCAACAGGGACCCTGAACTTGACCTTCCCAGAGAAGCCAGGGCCTCCCTT GCATCTGCCCCAATGCCCAGCATGCCGCCAAGGGAGTGCAAAATCAACCCCACTTTGACTCCTTGGAGAG GTAGCAGCCTTTCACCTGACACATTTTACTTGTTCAGATTAAGTCCATTACAGACAAGCACAGGACTCTT TTTTTTTTTCTTCTTTTTTTTTTCCAGAAAGGTCCCCACTAGAGGTTTTTGTTTTGTTTTATTTTTAATT TAAAAAAGCGTGACGCCAACAGCCCTGGCCTCATTCGCTTGCTTCTGCCTGGCCCTTGTCAACACAGTCC TTGGCAACTGTCCCTGACCCAGATATGCACAGACTGGGTGCCTGTGACTTCCTCTGCCGCCATAGCTCTG CAGTTCACCTGAGTGCTGACAGGCTAGAAGTGCTTGCTCGTCCGCAGCCACAGCGGCCTGTTGAGCTGGT TCTCCAAGGCTGCCTGCCATCTCCTCGAGGAGACAGCTGCTGTCTGCACCCTGTCCTTGACACAGTGTCC TGTGTTGAGCCCCAGTGCCTTTAGTCCAGGCCCTTTGTGGGAAGGCAGAGCCTAACCCTTGGAGGCTCTG TGTTGTTGCCTTCTGTCTGAGCTACCTACGATGTTCAAAGAGCCCAGATTCCTCCTGCAATGGGGAGAGA GGCCTCCTTGAGATTAGTGTCCCTCCAGTCTGAGCAGGAACTTAACCTTTTCCCCCATAGCAGCAGCCCC TCGGGCTCCTTTGTTTTGTTTTGTTTTGTTAATATGTTGGAGTTAATTGAACTGATTTTATTGAAGTGTG TGTTGCTGTTGCATTAAAAGGTTTTCTTCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4187356 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4187356 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17465 same embryo
	EurExpress:euxassay_003916 same experiment
	MGI:4828868 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source