EMAGE mouse gene expression spatial database

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EMAGE:17497

Irak1bp1 interleukin-1 receptor-associated kinase 1 binding protein 1 ( MGI:1929475)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003939. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003939_01	euxassay_003939_02	euxassay_003939_03	euxassay_003939_04

euxassay_003939_05	euxassay_003939_06	euxassay_003939_07	euxassay_003939_08	euxassay_003939_09

euxassay_003939_10	euxassay_003939_11	euxassay_003939_12	euxassay_003939_13	euxassay_003939_14

euxassay_003939_15	euxassay_003939_16	euxassay_003939_17	euxassay_003939_18	euxassay_003939_19

euxassay_003939_20	euxassay_003939_21	euxassay_003939_22	euxassay_003939_23	euxassay_003939_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17497_wholemount_strong.wlz

17497_wholemount_moderate.wlz

17497_wholemount_weak.wlz

17497_wholemount_possible.wlz

17497_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17497_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9758
Entity Detected:	Irak1bp1, interleukin-1 receptor-associated kinase 1 binding protein 1 ( MGI:1929475)
Sequence:	sense strand is shown >T9758 GCCCCAGCTATGTCGCTGCAACCCGCCCCTGCATCGCGGGTGTTCATGGAACTGGTTCCCTGGGCTGACC GGGGCCGGGAAAACCATCCGATCTCGGCCGCGGAGGCGCAGCCGATCGGCCGCCGACCCCACGTCGCGGA GGCCCACCCCGGAGCCCGTGAGGTCCACGTGAGCGGCGCGGCCGAGGTGTCCGCCAGCCCCGACCGAGCG CTGGTGACCGTGCGAGTGAGCAGCACCAAGGAGGTGTCGGCCGAGGCCAAGAAGAGTGTGTGTCGCCGCC TGGACTACATCACGCAGAGCCTCCAGCAGCAGGGGTTTCAGGCAGAAAATGTAACTGTGACAAAGAACAT TAGAAGAGTAGAAAATGCGTATCACATGGAAGCCGAGGTCTGCATTACATTTACTGAATTTGGAAAAATG CAAAATATTTGTAACTTTCTGGTTGAAAAACTAGATAGCTCTGTTGTCATCAGCCCACCTGAGTTCTATC ACACACCAGGTTCTGTTGAGAATCTTCGGCGGCAGGCCTGTCTGGTTGCTGTTGAGAATGCATGGCGCAA AGCTCAGGAAGTCTGTGACCTCGTTGGCCAAACTCTGGGAAAACCTTTACTAATCAAAGAAGAAGAAACA AAAGACTGGGAAGGCCAAACGGATGATCATCAGTTATCCAGACTGCCAGGCACATTAACTGTACAACAGA AAATCAAAAGTGCAACCATCCATGCAGCTTCGAAGGTGTTCATTACTTTTGAGGTAAAAGGAAAAGAGAA GAAAAAAAAGCATCTTTAAAATTCTAACTAAAACATGATGTGTCTGTACTTTTAAATCTGTTTTTATACA TTTTTACAGTGCTTCTTTCTGTCTCGGACATTCAACATATTGTATGGCATATTAAGTAAGCCTCAGTAAA ATCTGCCACTTTTTGAACATAATGCCACAGAATATATACGATTACTTTCTAGTTTCCAGAACACTCAGAG AAACGCTGTAAGAAATAAATGTATTAAAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6742251 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6742251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17495 same embryo
	EMAGE:17496 same embryo
	EurExpress:euxassay_003939 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source