EMAGE mouse gene expression spatial database

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EMAGE:17498

Dync1i2 dynein cytoplasmic 1 intermediate chain 2 ( MGI:107750)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005868. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005868_01	euxassay_005868_02	euxassay_005868_03	euxassay_005868_04

euxassay_005868_05	euxassay_005868_06	euxassay_005868_07	euxassay_005868_08	euxassay_005868_09

euxassay_005868_10	euxassay_005868_11	euxassay_005868_12	euxassay_005868_13	euxassay_005868_14

euxassay_005868_15	euxassay_005868_16	euxassay_005868_17	euxassay_005868_18	euxassay_005868_19

euxassay_005868_20	euxassay_005868_21	euxassay_005868_22	euxassay_005868_23	euxassay_005868_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17498_wholemount_strong.wlz

17498_wholemount_moderate.wlz

17498_wholemount_weak.wlz

17498_wholemount_possible.wlz

17498_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17498_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 04 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 19

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2905
Entity Detected:	Dync1i2, dynein cytoplasmic 1 intermediate chain 2 ( MGI:107750)
Sequence:	sense strand is shown >T2905 TGGCCTCGNAGGCCAGATTCGGCACGAGGCTTTCCCATCCACAGGATAGCATGGAGTTGGTTCATAAACA GTCAAAGGCAGTAGCTGTAACATCTATGTCTTTCCCTGNTGGAGACGTCAACAACTTTGTTGTGGGCAGT GAAGAAGGTTCTGTGTATACAGCATGCCGCCATGGCAGCAAAGCTGGAATCAGTGAGATGTTTGAGGGAC ACCAGGGACCCATCACTGGAATCCACTGTCACGCGGCTGTTGGAGCAGTAGACTTCTCACATCTGTTTGT CACTTCGTCTTTTGACTGGACAGTTAAACTTTGGACAACTAAGAATAACAAGCCTTTGTACTCATTTGAA GATAATTCAGACTATGTCTATGATGTTATGTGGTCACCCACCCACCCAGCCCTGTTTGCCTGTGTGGACG GCATGGNGAGACTGGATCTATGGAATCTCNACAATGACACAGAGGTACCAACTGCCAGCATTTCTGTGGA GGGCAACCCTGCTCTTAACCGTGTGAGATGGACCCACTCAGGAAGAGAAATTGCCGTGTGTGATTCTGAA TGACAGATTGNCATCTATGATGTGGGA
Notes:	The probe template was PCR amplified from IMAGE:1531233 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1531233 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17500 same embryo
	EMAGE:17499 same embryo
	EurExpress:euxassay_005868 same experiment
	MGI:4824429 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source