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EMAGE:17532

Eif4ebp3 eukaryotic translation initiation factor 4E binding protein 3 ( MGI:1270847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17532
euxassay_005869_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17532Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17532_wholemount_strong.wlz
17532_wholemount_moderate.wlz
17532_wholemount_weak.wlz
17532_wholemount_possible.wlz
17532_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17532_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9781
Entity Detected:Eif4ebp3, eukaryotic translation initiation factor 4E binding protein 3 ( MGI:1270847)
Sequence:sense strand is shown

>T9781
AGGTGGGTGGAGCCCTCAAGTTGACTGGCCAGTGGCCTGCCCCGGGTGTAACATATTCTAGTTGTTGCCT
GCTGCGACTGCCTACACCTCAGAGCTGTTTTCCACGCTTTCAAGGCACCAGCTCTTGCCTTCCTGCTGCT
ATGTCCTCGTCCACGAGTTGCCCAATTCCAGGGTGCCGGGACCAGCTGCCAGACGGCTACAGCACCACGC
CTGGCGGCACACTATACGCCACTACCCCGGGAGGCACCAGGATCATCTACGACAGAAAGTTCCTGCTGGA
GTGCAAGAACTCACCCATTGCCCGGACACCTCCCTGCTGCCTCCCTCAGATTCCCGGGGTCACAACTCTC
CCAGCAGTACCACCCTCCAAGTTGGAGTTGCTGAAGGAACAGAAGCAGACAGAGGTAGAAATAACCGATG
ACGAACAGTTTGAAATGGACATGTAATCTAGTGCAAAATGACCTGTATGGAGTAATGGAAGGAGCTCTCA
TCCTACTATTGCCATCACCTCTTCCTGGGTATCCAAGGGCCAGCTGGCCTCATCTAATCCAGAAAAGAAT
GACTTGTTGGTTCTGGGCTTCCTTTAGTTGAGGCAGCTAGACCAGGGATAGCAGCGGGCAACCTGCCAAG
CCTTTAGCTTTCTCTGGTCTATACATATTCCTCCCAAACCTCTTCCCCTCAGGGCTGGCACTGGGGAACA
GAGTATGTCACCTTCTGGCTGCTTAGTAAACTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6743910 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6743910 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17534 same embryo
 EMAGE:17533 same embryo
 EMAGE:17530 same embryo
 EMAGE:17531 same embryo
 EMAGE:17529 same embryo
 EurExpress:euxassay_005869 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS