EMAGE mouse gene expression spatial database

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EMAGE:17534

Tuba3b tubulin, alpha 3B ( MGI:1095408)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005855. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005855_01	euxassay_005855_02	euxassay_005855_03	euxassay_005855_04

euxassay_005855_05	euxassay_005855_06	euxassay_005855_07	euxassay_005855_08	euxassay_005855_09

euxassay_005855_10	euxassay_005855_11	euxassay_005855_12	euxassay_005855_13	euxassay_005855_14

euxassay_005855_15	euxassay_005855_16	euxassay_005855_17	euxassay_005855_18	euxassay_005855_19

euxassay_005855_20	euxassay_005855_21	euxassay_005855_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17534_wholemount_strong.wlz

17534_wholemount_moderate.wlz

17534_wholemount_weak.wlz

17534_wholemount_possible.wlz

17534_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17534_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 05 14

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 16

trigeminal v nerve

strong

regional

strong expression: see section 08 15

spinal cord

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 12

cervical ganglion

strong

regional

strong expression: see section 06

thoracic ganglion

strong

regional

strong expression: see section 08 10

dorsal root ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

not examined

homogeneous

not examined expression: see section 01 02 03 21 22

neural retina

strong

regional

strong expression: see section 01 02 03 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9798
Entity Detected:	Tuba3b, tubulin, alpha 3B ( MGI:1095408)
Sequence:	sense strand is shown >T9798 GAGGAGCAGCTCTGAGGATCGGCTGAGGTGCTCTGCTAGGAGTTCAGCATGCGTGAGTGTATCTCTATCC ACGTGGGGCAGGCAGGTGTCCAGATTGGCAATGCCTGCTGGGAGCTGTACTGCCTTGAACATGGTATTCA GCCTGACGGTCAGATGCCAAGCGACAAAACCATTGGCGGCGGGGACGACTCATTCAACACATTCTTCAGT GAGACTGGAGCCGGCAAGCACGTGCCCAGGGCAGTGTTTGTGGACCTGGAGCCCACTGTGGTCGATGAAG TACGCACCGGAACCTACCGGCAGCTTTTCCACCCAGAGCAGCTCATCACTGGGAAGGAAGATGCAGCCAA CAATTATGCCAGAGGTCATTATACCATTGGCAAGGAGATTGTTGACCTGGTCCTGGACCGGATCCGAAAA CTGGCCGATCTGTGCACGGGACTGCAGGGCTTCCTCATCTTCCACAGCTTTGGAGGTGGCACAGGGTCTG GGTTTGCATCGCTGCTGATGGAGCGGCTTTCAGTGGACTATGGCAAGAAGTCCAAGCTGGAGTTTGCCAT CTACCCAGCCCCCCAGGTTTCTACAGCGGTCGTGGAGCCTTACAACTCCATCCTGACCACGCACACCACC CTGGAGCATTCCGACTGTGCTTTCATGGTGGATAACGAAGCCATCTACGACATCTGCCGGCGCAACCTGG ATATTGAACGTCCCACATACACCAACCTCAACCGTCTGATTGGGCAGATTGTGTCATCCATCACAGCCTC CCTAAGGTTCGATGGCGCCCTGAATGTGGACTTAACAGAATTCCAGACCAACCTGGTGCCATACCCTCGC ATCCACTTCCCACTGGCGACCTATGCCCCAGTCATCTCTGCTGAGAAGGCATACCATGAGCAGCTGTCAG TAGCAGAGATCACCAATGCTTGCTTCGAGCCAGCCAATCAGATGGTCAAGTGTGACCCTCGCCATGGCAA ATACATGGCCTGCTGCATGTTGTACCGGGGGGATGTGGTTCCCAAAGATGTCAATGCGGCTATTGCCACC ATCAAGACCAAGCGCACCATCCAGTTTGTAGATTGGTGCCCGACTGGATTTAAGGTGGGTATTAACTACC AGCCTCCTACTGTGGTCCCTGGGGGAGACCTGGCCAAGGTGCAGCGGGCTGTGTGCATGCTGAGCAATAC CACGGCCATCGCAGAGGCCTGGGCCCGCCTGGACCACAAATTTGACCTCATGTACGCCAAGCGGGCCTTT GTGCATTGGTACGTGGGAGAAGGCATGGAGGAAGGGGAGTTCTCCGAGGCCCGGGAGGACCTGGCAGCGC TGGAGAAGGACTATGAAGAGGTGGGCGTGGATTCCGTGGAAGCAGAGGCAGAAGAAGGGGAGGAGTACTG AGCGCATGGGTCTGGCTGGCGGCCGTCCATTTACACCTTCCCCACCATTGGAAATAAAGGATATGTTTAC TTATTAAAGTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6771187 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6771187 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17533 same embryo
	EMAGE:17530 same embryo
	EMAGE:17532 same embryo
	EMAGE:17531 same embryo
	EMAGE:17529 same embryo
	EurExpress:euxassay_005855 same experiment
	MGI:4829005 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source