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EMAGE:1754

Pax5 paired box gene 5 ( MGI:97489)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1754
0267b.jpg This embryo was treated with ProteinaseK for 30min.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1754Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1754_wholemount_strong_3D_1.wlz
1754_wholemount_moderate_3D_1.wlz
1754_wholemount_weak_3D_1.wlz
1754_wholemount_possible_3D_1.wlz
1754_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1754_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
Expression was detected in dorsal and ventral mesencephalon.
future hindbrain
detected detected
Expression was detected in dorsal and ventral rhombencephalon.
future spinal cord
detected detected
regionalExpression was detected in ventral spinal cord.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506123
Entity Detected:Pax5, paired box gene 5 ( MGI:97489)
Sequence:sense strand is shown

>MGI:3506123
CCCAGATGTAGTCCGCCAAAGGATAGTGGAACTTGCCCATCAAGGTGTCAGGCCCTGCGACATCTCCAGG
CAGCTTCGGGTCAGCCATGGTTGTGTCAGCAAAATTCTTGGCAGGTATTATGAGACAGGAAGCATCAAGC
CGGGGGTGATTGGAGGATCCAAACCAAAGGTTGCCACTCCCAAAGTGGTGGAAAAAATCGCTGAGTACAA
ACGCCAAAACCCTACCATGTTTGCCTGGGAGATCAGGGACCGGCTGTTGGCAGAGCGAGTCTGTGACAAT
GACACTGTGCCCAGCGTCAGCTCCATCAACAGGATCATTCGGACAAAAGTACAGCAGCCCCCCAATCAGC
CGGTCCCAGCTTCCAGTCACAGCATAGTGTCTACAGGCTCCGTGACGCAGGTGTCATCGGTGAGCACCGA
CTCCGCGGGCTCCTCATACTCCATCAGTGGCATCCTGGGCATCACGTCCCCCAGTGCCGACACCAACAAA
CGCAAGAGGGATGAAGGTATTCAGGAGTCTCCAGTGCCGAATGGCCACTCACTTCCGGGCCGGGACTTCC
TCCGGAAGCAGATGCGGGGAGACCTGTTCACACAGCAGCAGCTGGAGGTGCTGGACCGCGTGTTTGAGAG
ACAGCACTACTCTGACATCTTCACCACCACGGAACCCATCAAGCCAGAACAGACCACAGAGTATTCAGCC
ATGGCTTCACTGGCTGGAGGCCTGGATGACATG
nt 131 - nt 863 of NM_008782.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: ccggaattcccagatgtagtccgccaaagg 3'primer: cccaagcttcatgtcatccaggcctccagcc
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508829 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE