EMAGE mouse gene expression spatial database

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EMAGE:17551

Anxa3 annexin A3 ( MGI:1201378)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005881. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005881_01	euxassay_005881_02	euxassay_005881_03	euxassay_005881_04

euxassay_005881_05	euxassay_005881_06	euxassay_005881_07	euxassay_005881_08	euxassay_005881_09

euxassay_005881_10	euxassay_005881_11	euxassay_005881_12	euxassay_005881_13	euxassay_005881_14

euxassay_005881_15	euxassay_005881_16	euxassay_005881_17	euxassay_005881_18	euxassay_005881_19

euxassay_005881_20	euxassay_005881_21	euxassay_005881_22	euxassay_005881_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17551_wholemount_strong.wlz

17551_wholemount_moderate.wlz

17551_wholemount_weak.wlz

17551_wholemount_possible.wlz

17551_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17551_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

interdigital region between hindlimb digits 1 and 2

weak

regional

weak expression: see section 08 09 22

interdigital region between hindlimb digits 2 and 3

weak

regional

weak expression: see section 08 09 21 22

interdigital region between hindlimb digits 3 and 4

weak

regional

weak expression: see section 08 09 22

interdigital region between hindlimb digits 4 and 5

weak

regional

weak expression: see section 08 09 21

diencephalon meninges

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

telencephalon meninges

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain meninges

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

rectum

moderate

regional

moderate expression: see section 12

meckel's cartilage

moderate

regional

moderate expression: see section 11 weak expression: see section 07 09 10 12 13 14 15 16 17 18 19

nucleus pulposus

weak

regional

weak expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2913
Entity Detected:	Anxa3, annexin A3 ( MGI:1201378)
Sequence:	sense strand is shown >T2913 GGCCTCGAGGCCAGATTCGGCACGAGGATCTCGCAGGCCTATTATACAGTGTATAAGAAGAGCCTCGGGG ATGACATTAGCTCTGAGACGTCTGGAGACTTCCGGAAAGCTCTGCTGACTCTGGCAGATGGTAGAAGAGA TGAAAGCCTCAAAGTGGATGAACATCTGGCCAAAAAGGATGCTCAGGTAGATCCTCTATAATGCTGGTGA GAACAAATGGGGCACAGACGAAGACAAATTCACCGAGGTCTTGTGTCTACGGAGCTTCCCGCAGCTGAAA CTAACATTTGATGAGTACAGAAATATTAGCCAGAAGGACATTGAGGACAGCATTAAAGGAGAATTATCTG GACATTTTGAAGACCTGCTGCTGGCCATAGTTCATTGTGCGAGGAACACTCCAGCGTTTTTGGCAGAAAG ACTTCATCAGGCTTTGAAGGGTGCTGGAACAGATGAATTCACTCTGAACAGAATAATGGTTTCCAGATCA GAGATTGATCTTTTGGACATCAGACATGAGTTCAAGAAGCACTATGGCTACTCTCTATACTCGGCCATCC AATCAGATACTTCTGGAGACTACAGAACCG
Notes:	The probe template was PCR amplified from IMAGE:1531374 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1531374 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17549 same embryo
	EMAGE:17547 same embryo
	EMAGE:17550 same embryo
	EMAGE:17548 same embryo
	EurExpress:euxassay_005881 same experiment
	MGI:4823152 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source