EMAGE mouse gene expression spatial database

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EMAGE:17569

Tmed6 transmembrane emp24 protein transport domain containing 6 ( MGI:1913519)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005905. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005905_01	euxassay_005905_02	euxassay_005905_03	euxassay_005905_04

euxassay_005905_05	euxassay_005905_06	euxassay_005905_07	euxassay_005905_08	euxassay_005905_09

euxassay_005905_10	euxassay_005905_11	euxassay_005905_12	euxassay_005905_13	euxassay_005905_14

euxassay_005905_15	euxassay_005905_16	euxassay_005905_17	euxassay_005905_18	euxassay_005905_19

euxassay_005905_20	euxassay_005905_21	euxassay_005905_22	euxassay_005905_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17569_wholemount_strong.wlz

17569_wholemount_moderate.wlz

17569_wholemount_weak.wlz

17569_wholemount_possible.wlz

17569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10030
Entity Detected:	Tmed6, transmembrane emp24 protein transport domain containing 6 ( MGI:1913519)
Sequence:	sense strand is shown >T10030 AGCTCACCTGCAGAAGAACCCACAGAAGGCAGCATGTTCCCTTTGCTCCTCGTGGCTGAGCTGGTAGTTC TGAGCCTCGTGACTTCTGTTAAGAGCCAGGAGACAGATCCCCTACACGGCTCCAAGGACCAGCCACTCTT CCGTGGAGCTGATCGGAATGACTTTGCCATCGTGGTCTCTCCAGGAGCCATCGAATGTTTCTGGCAATTT GCTGACCAGATGGGATACCTCTACTTCAGCTACGAGGTTCAGCGCATTCTCGGGATGTCACATGACCGCC ACATTGTTGCCACGGCACATACCCCGCAGGGTTTCCTTATTGACACTTCCCAGGATGTAAGGGGCCAGAT TAACTTTGCTACACAAGAAACAGGCTTCTATCAGCTTTGTCTAAAAAATGAGCAGAATCGCTTCAGTTCT ATACAAGTGTATCTCAACTTCGGAGTCTTCTATGAGGGGCCCGAGGTGGATCACAAGCAGAGTCAAAGGA AGCAACTGAACGACACTCTGGATGCGATTAAGGACAGCACACAAAGGGTGGAGAACCAAGTTTTCCACAT GTGGCGCTTCTACAACTATGCCAGGATGAGAAAAGTGGCTGACTTCTTCCTCCTGCAGTCTAACTACACC TATGTGAACTGGTGGTCCACAGCTCAGAGCCTTGCTATTGTTCTTTCCGGGGCCCTCCAGCTCTATTTCT TGAAAAGACTTTTTACTGCTTCAACTACTGACACAAAGAAGCCAAGATGCTAACATATAGCCTATTTGTC AAAGCTTTGATCAAATGGGCTTTGGAAAGTTATTGGGGGGGGGGTCAACTTATGTCCCCTTTCTCTTGTG TCCACCAATAAAGGCAAATGTTTAACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6760985 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6760985 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17568 same embryo
	EMAGE:17571 same embryo
	EMAGE:17567 same embryo
	EMAGE:17570 same embryo
	EurExpress:euxassay_005905 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source