EMAGE mouse gene expression spatial database

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EMAGE:17572

Serpind1 serine (or cysteine) peptidase inhibitor, clade D, member 1 ( MGI:96051)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005919_01	euxassay_005919_02	euxassay_005919_03	euxassay_005919_04	euxassay_005919_05

euxassay_005919_06	euxassay_005919_07	euxassay_005919_08	euxassay_005919_09	euxassay_005919_10

euxassay_005919_11	euxassay_005919_12	euxassay_005919_13	euxassay_005919_14	euxassay_005919_15

euxassay_005919_16	euxassay_005919_17	euxassay_005919_18	euxassay_005919_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17572_wholemount_strong.wlz

17572_wholemount_moderate.wlz

17572_wholemount_weak.wlz

17572_wholemount_possible.wlz

17572_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17572_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10041
Entity Detected:	Serpind1, serine (or cysteine) peptidase inhibitor, clade D, member 1 ( MGI:96051)
Sequence:	sense strand is shown >T10041 ATCTCTGCCACACCACTGCTCAGAGGTACAGAGATAGCTGGTGTGAGCATTGGTGCTGTGAAGGCTTCTT GCTGTGTTAAGAGAATCTGCTGTCTGCACATTCCAGTGGGGGTGGTGTCAGCCAGGCTCCCTGCCATTCC TTGTTGTCTGTTTTTCACAGCAGCTTTAGCTCTGCAGAAATGAAACATCCACTCTGCACTCTTCTTTCTC TCATCACATTTATGTGTATTGGGAGCAAAGGCCTTGCTGAGCAGCTAACTAATGAAAACCTGACCACGTC CTTCCTGCCTGCTAACTTCCACAAAGAAAACACAGTCACCAATGACTGGATCCCAGAGGGGGAAGAGGAT GAGGACTACCTGGACCTGGAGAAGCTGCTTGGTGAAGATGACGACTACATTTATATCATCGATGCCGTTT CCCCGACAGACTCAGAATCAAGTGCTGGCAACATCCTGCAGCTTTTCCAAGGCAAGAGCCGGATCCAGCG TCTTAATATCCTCAATGCAAAGTTTGCCTTCAACCTTTACCGAGTCCTGAAGGACCAGGCTACCACATCT GATAACCTCTTCATAGCACCTGTTGGCATTTCTACTGCCATGGGGATGATCTCCTTAGGTCTGAGGGGAG AGACCCATGAAGAAGTACACTCAGTTCTACACTTCAGAGACTTTGTCAATGCTAGCAGCAAGTACGAGGT TACCACCATTCACAATCTCTTTCGAAAGCTGACCCATCGTCTATTCAGGAGGAATTTTGGGTACACACTT CGGTCAGTTAATGGCCTTTATATTCAGAAGCAGCTTCCCATACGGGAGGACTTCAAAGCTGCTATGAGAG AGTTTTACTTTGCTGAGGCCCAGGAGGCTAACTTCCCAGATCCTGCCTTCATATCAAAGGCAAACAACCA CATTCTGAAACTCACCAAGGGCCTTATAAAAGAAGCTCTGGAGAATATAGATCCTGCTACTCAGATGCTG ATTCTGAACTGCATCTACTTCAAAGGAACTTGGGTGAATAAATTCCCAGTAGAAATGACGCACAACCACA ACTTCCGGCTGAATGAGAGAGAAGTAGTCAAAGTCTCCATGATGCAAACTAAAGGGAATTTCCTTGCGGC AAATGACCAGGAGCTAGACTGTGACATTCTCCAGCTGGAGTACGTAGGGGGCATCAGCATGCTTATTGTA GTTCCACGGAAGCTATCGGGAATGAAGACTCTTGAAGCACAGCTTACACCCCAGGTGGTGGAGAGATGGC AAAAAAGCATGACAAACAGAACTCGAGAGGTACTTCTGCCAAAGTTTAAGCTTGAGAAGAATTACAACCT GGTGGAGGTCCTTAAGTCCATGGGAATCACAAAGCTCTTCAACAAGAATGGCAATATGTCAGGCATCTCA GACCAGAGGATCGCCATTGACCTGTTCAAGCACCAAAGTACCATCACAGTGAATGAAGAGGGTACCCAAG CTGCAGCTGTAACTACAGTGGGGTTCATGCCGCTGTCTACCCAAGTCCGATTCACTGTCGACCGTCCCTT CCTGTTCCTAGTGTATGAGCACCGAACCAGCTGTCTGCTCTTCATGGGAAAAGTGACCAACCCTGCCAAG TCTTAATGGCAGTGGTCTGGGCACCTGATGTAGTTTGGGGACACTCTGTATTCCTGTTTTCATTCTAACA AAGAGGACAGAGCTATTTTGCTATGATAATCTCTGTGGTTTATGATAATAATCTGAACAGCAAACAGTGC CATTAAGTGCACAGATGTTAATGCAACTAATGGTTGTGCCAGCTATTCCCTAGAGGTCCTAGACACACAG ACTGCCTTAGCCCCCACACCTCTGTCCCTAGGAGAATGAATGGGCAGAGTGGAGCTGACACTCTCTCCTC AGGCAGCCTCCTCAGCCTTTATTCGTCAACGGCCTCTGCTCAGCCAGGCCCTTCCAGACAAAAGGCACTG AGGAACTTGCACCCCTTCCAGGGAGGGTGTCCAAGTAGCACCGTTCCTGCCCAGTGGGAAAGCAATCCCA ACATGTCTGAGATGTTCGGACTTTTAAATTCATGTAAATATCTCCTCTTCTGCTGTGTGTACATAGGACT ACATAGTCTATCCTAGAGATAAATAAACAACCATATTTACCATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6768311 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6768311 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827998 same experiment
	EurExpress:euxassay_005919 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source