EMAGE mouse gene expression spatial database

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EMAGE:17578

Rab38 RAB38, member of RAS oncogene family ( MGI:1919683)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005916. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005916_01	euxassay_005916_02	euxassay_005916_03	euxassay_005916_04

euxassay_005916_05	euxassay_005916_06	euxassay_005916_07	euxassay_005916_08	euxassay_005916_09

euxassay_005916_10	euxassay_005916_11	euxassay_005916_12	euxassay_005916_13	euxassay_005916_14

euxassay_005916_15	euxassay_005916_16	euxassay_005916_17	euxassay_005916_18	euxassay_005916_19

euxassay_005916_20	euxassay_005916_21	euxassay_005916_22	euxassay_005916_23	euxassay_005916_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17578_wholemount_strong.wlz

17578_wholemount_moderate.wlz

17578_wholemount_weak.wlz

17578_wholemount_possible.wlz

17578_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17578_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18

skin

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 22 23 24 moderate expression: see section 02 03

anterior naris

moderate

regional

moderate expression: see section 13 14 16

external naris

moderate

regional

moderate expression: see section 12 13

naso-lacrimal duct

strong

regional

strong expression: see section 06 08 20 moderate expression: see section 05 07 17 18 19 21 22 23

esophagus epithelium

strong

regional

strong expression: see section 06 07 08 09 10

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

right lung

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

axial skeleton

strong

regional

strong expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10033
Entity Detected:	Rab38, RAB38, member of RAS oncogene family ( MGI:1919683)
Sequence:	sense strand is shown >T10033 ACATAGAGCTCCGGTCTCCGTCGGTGCCCAGCCCCGGCTGTGCTTCCCAGAGCAAGCTCCAGGCTCCGCA AGACCCGCGGGCCTCCAGGATGCAGACACCTCACAAGGAGCACCTGTACAAGCTGCTGGTGATCGGCGAC CTGGGTGTGGGCAAGACCAGCATTATCAAGCGCTATGTGCACCAAAACTTCTCCTCGCACTACCGGGCCA CCATTGGTGTGGACTTCGCGCTGAAGGTGCTCCACTGGGACCCAGAGACGGTGGTGCGCTTGCAGCTCTG GGACATTGCTGGTCAAGAAAGATTTGGAAACATGACAAGAGTTTATTACCGGGAAGCTATGGGGGCATTT ATTGTTTTTGATGTCACCAGACCAGCCACATTTGAAGCCGTGGCAAAGTGGAAAAATGATTTGGACTCAA AGTTAACGCTCCCTAATGGTAAGCCAGTGTCAGTGGTTCTGTTGGCCAACAAATGTGACCAAGGGAAGGA TGTGCTTATGAACAATGGACTCAAGATGGACCAGTTCTGCAAGGAGCATGGCTTCGTAGGATGGTTTGAA ACATCAGCCAAGGAAAACATAAACATTGATGAAGCCTCAAGATGCCTGGTCAAGCACATACTTGCAAATG AGTGTGACCTCCTAGAGTCTATAGAACCGGACATTGTGAAGCCCCATCTCACATCGCCCAAGGTTGTCAG CTGCTCTGGCTGTGCCAAATCCTAGAAGGCTCCTCTGCTGGCATATGACAGACAGAACCCGTGGCCCTCA TGAATCGTGCTTCAGTTTTTCCTTATTACCATTTTGGGTAAGCGTCAGGATAGGGAAGCACATGTGACAA GCCAAAGATACATGACTGTATGATTCCTGTCAAAGAGGAACAGCAAATGTTCTTTATGTGTTTTCCCACC CCATCAGCACAGTGTTTACAAGCTTTTAAAATATTAGTCTGTCACAATATGCTGTTTTATCATTGAGCAA AGCCACTCAGGGGCACAGACAGCCCTAATATTTGTTCCTTTAAATCAACAAAGGCTTCTGGTCTTCTTGA GAAGGGGAATAACAGAGCAAGGCAGAGGTCAAGCTAAGTGTGGGGATTTGTCTTGCCCTGGTGTGTCTTT GTTCAGGTATCAATTTGTTCCCGGGTGGTCTGATAGGTCTATTAAATAGAAACCATTCATGGTAGACCTA AGGGTTGTCTGTGATGTTTCTCTTCAGAGTCGTGTGCACAGGCAGCCTGGGCTTTTGTTGTCACTTGCTG TGCCCTGAATGCTGGTTTAACTGAAAACTGTATGGAAAGATCTGCTCCCTGTATGTGCCTTTCTTTCAGC TTCCTCTGACTCAAGCTGCAGGACTCTTCTGTATGTGGAAGATATATTATATATATTTTTCACAAGTGAA AAATAAAACATTAAAAATGCTGTTTCCCTGTTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6762755 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6762755 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17579 same embryo
	EMAGE:17581 same embryo
	EMAGE:17580 same embryo
	EMAGE:17582 same embryo
	EMAGE:17577 same embryo
	EurExpress:euxassay_005916 same experiment
	MGI:4827576 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source