EMAGE mouse gene expression spatial database

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EMAGE:17606

Papss2 3'-phosphoadenosine 5'-phosphosulfate synthase 2 ( MGI:1330223)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005940. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005940_01	euxassay_005940_02	euxassay_005940_03	euxassay_005940_04

euxassay_005940_05	euxassay_005940_06	euxassay_005940_07	euxassay_005940_08	euxassay_005940_09

euxassay_005940_10	euxassay_005940_11	euxassay_005940_12	euxassay_005940_13	euxassay_005940_14

euxassay_005940_15	euxassay_005940_16	euxassay_005940_17	euxassay_005940_18	euxassay_005940_19

euxassay_005940_20	euxassay_005940_21	euxassay_005940_22	euxassay_005940_23	euxassay_005940_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:17606

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17606_wholemount_strong.wlz

17606_wholemount_moderate.wlz

17606_wholemount_weak.wlz

17606_wholemount_possible.wlz

17606_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17606_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

humerus

strong

regional

strong expression: see section 04 05 20 21 22 23 24

hand

strong

regional

strong expression: see section 23 24

forelimb digit 1

strong

regional

strong expression: see section 01 02 03 04 05

forelimb digit 2

strong

regional

strong expression: see section 01 02 03 04 05

forelimb digit 3

strong

regional

strong expression: see section 01 02 03 04 05

forelimb digit 4

strong

regional

strong expression: see section 01 02 03 04 05

forelimb digit 5

strong

regional

strong expression: see section 01 02 03 04 05

foot

strong

regional

strong expression: see section 05

hindlimb digit 1

strong

regional

strong expression: see section 06 07 08 09

hindlimb digit 2

strong

regional

strong expression: see section 06 07 08 09

hindlimb digit 3

strong

regional

strong expression: see section 06 07 08 09

hindlimb digit 4

strong

regional

strong expression: see section 06 07 08 09

hindlimb digit 5

strong

regional

strong expression: see section 06 07 08 09

fibula

strong

regional

strong expression: see section 01 02 03 04

tibia

strong

regional

strong expression: see section 01 02 03 04

femur

strong

regional

strong expression: see section 04 07 08 09 18 19 20 21 22 23 24

naris

strong

regional

strong expression: see section 12 13 14 15 16 17

nasal septum

moderate

regional

moderate expression: see section 12 14 15

viscerocranium

strong

regional

Expression in the turbinate bone.

heart valve

strong

regional

strong expression: see section 11 12 13 14

hyoid

strong

regional

strong expression: see section 09 10 11 12 13 14

meckel's cartilage

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

axial skeleton

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

basioccipital bone

strong

regional

strong expression: see section 01 02 03 04 19 20 21 23 24 not examined expression: see section 09 10 11 12 13 14

basisphenoid bone

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 22 23 24 not examined expression: see section 01 02 03 04 05 06 07 08 19 20 21

exoccipital bone

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23

vault of skull

strong

regional

strong expression: see section 01 02

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 18 19 20 21

clavicle

strong

regional

strong expression: see section 09 16

scapula

strong

regional

strong expression: see section 20

pelvic girdle skeleton

strong

regional

strong expression: see section 10 11 16 17 18

axial skeleton tail region

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10059
Entity Detected:	Papss2, 3'-phosphoadenosine 5'-phosphosulfate synthase 2 ( MGI:1330223)
Sequence:	sense strand is shown >T10059 ATTCCCATTCACACCCACCTCACAGACCTTCACAGACTCTGCAGCTCATTCATTCACCCCAATGGCCAGC AAAGCCAGTTTGTAACCGAGTATTCTCAACATCAGATATCATGTCTTGGAGGAAGTTACCTAAACTCTGA AGAATTATCATGTCTGCAAATTTCAAAATGAACCATAAAAGAGACCAGCAAAAATCCACCAATGTGGTCT ACCAGGCCCATCATGTGAGCAGGAACAAGAGAGGACAAGTGGTTGGAACCAGGGGAGGATTCCGAGGATG TACCGTGTGGCTAACAGGTCTCTCTGGTGCTGGGAAAACAACCATAAGCTTTGCTTTGGAAGAGTACCTT GTATCTCACGCCATCCCATGTTACTCTCTGGATGGGGACAATGTCCGTCATGGCCTTAATAAGAACCTGG GATTCTCTGCGGGGGACCGAGAAGAGAATATCCGCCGGATCGCGGAGGTGGCCAGGCTCTTTGCCGACGC CGGCCTGGTTTGCATCACCAGCTTTATCTCTCCTTTTGCAAAGGATCGTGAGAATGCCCGAAAAATCCAC GAATCAGCAGGACTCCCATTCTTTGAGATCTTTGTAGATGCGCCTTTAAATATCTGTGAAAGCCGAGACG TAAAAGGACTCTACAAACGAGCCCGAGCAGGAGAGATTAAAGGGTTTACAGGCATCGATTCTGACTATGA GAAACCTGAAACTCCAGAGTGTGTGCTGAAGACCAACCTGTCTTCAGTAAGCGACTGTGTGCAACAGGTG GTGGAACTTTTGCAGGAGCAGAACATTGTACCCCACACCACCATCAAAGGCATCCACGAACTCTTTGTGC CAGAAAACAAAGTCGATCAAATCCGAGCTGAGGCAGAGACTCTCCCATCACTACCAATTACCAAGCTGGA TCTGCAGTGGGTGCAGATTCTGAGTGAAGGCTGGGCCACTCCCCTCAAAGGCTTTATGCGGGAGAAGGAG TACTTGCAAACTCTACACTTCGACACTCTACTGGACGGCGTGGTTCCCCGTGATGGAGTCATCAACATGA GTATTCCCATTGTATTGCCCGTTTCTGCGGATGACAAGGCACGGCTCGAAGGGTGCAGCAAATTTGCCTT GATGTACGAAGGTCGGAGGGTCGCTCTATTACAGGACCCTGAATTCTATGAGCATAGGAAAGAGGAGCGC TGTTCTCGTGTGTGGGGAACAGCCACTGCAAAGCACCCCCATATCAAAATGGTGATGGAAAGTGGGGACT GGCTTGTTGGTGGAGACCTACAGGTGCTAGAGAGAATAAGGTGGGACGATGGGCTGGACCAATACCGCCT TACGCCTCTGGAGCTCAAACAGAAGTGTAAAGACATGAATGCTGATGCCGTGTTTGCATTCCAGTTGCGC AATCCTGTCCACAATGGTCATGCCCTCCTGATGCAGGACACCCGCCGCAGGCTCCTGGAGAGGGGTTACA AGCACCCAGTCCTCCTGCTCCACCCTCTTGGGGGCTGGACCAAGGACGATGACGTACCTCTGGAATGGAG GATGAAACAGCATGCAGCTGTACTGGAGGAAAGGGTCCTGGATCCCAAGTCAACTATTGTTGCCATCTTT CCATCTCCTATGTTATATGCTGGTCCCACAGAGGTCCAGTGGCATTGCAGATGCCGGATGATTGCAGGAG CCAATTTCTACATTGTGGGTAGGGATCCCGCAGGAATGCCCCATCCTGAGACAAAGAAAGACCTATATGA ACCCACCCACGGGGGCAAGGTCTTGAGTATGGCCCCTGGCCTTACCTCTGTGGAAATAATTCCGTTCCGA GTGGCTGCCTACAATAAAATTAAAAAGGCCATGGACTTTTATGATCCAGCAAGGCACGAGGAGTTTGACT TCATCTCAGGAACTCGCATGAGGAAGCTCGCCCGGGAAGGAGAAGATCCCCCAGATGGCTTCATGGCCCC GAAAGCGTGGAAAGTGTTGACAGATTACTACAGGTCTCTGGAGAAGACCAACTAGGTGCTCCTGGCTCTG GCTTCTTCCTCAAGTGCTCTCTGACGATTTTTTTTTTCTATTTTTGTGATTTAGCTGCTCTGTATCCAAT TGCTTCTCGGTGACTTTTTAAAGCTAGTATTTTTGCAATGAAGTAAAAAGCTGTAACCATAATTTAAAAC CAAGTTCATTATGTCTATGAAGCTCACACTGGAGACTGAGTCTTAGGTGAAAGCAATATCGTTGTACGTT CCAGAATGAAAGCATTTGCACTGTACACTTCATCTCAGCCACTATGTCTCAGATTTCTTAAAAATGTAAC TGTGTGGCTAGCTGACTAGCCCAGAACAGAATCATACTTTCCAGCTTACTTGTGCCAGCCTGGAATCTCA ATGTCACTTACCAAAAGAAAACACACACACACACACACACACACACATACATACACACACACACACACAC ATATACATACACACACACTGTCGCTTTGAAGGAAATGTGTTATATCAGGACTCTTACTTCATAACTACAC ATACCATCACTGCCCAATGTACGAGCTGAAGATGCAACTGGAAGAAAAATTCATGTGAAGATGTAAATTA AGTCTTAAAGAACAATGTAATTTATGTCTGCCATGAAAATGTCATCATCCAATAGAGAGAGACGCTCTAA TGGCCTTCTATGAATAGTTACTCTAGTTTGGCTGGCACTTTTCAAAAGCATGGTATGCTTGGATCAAAAC AACCCCCCCCCCAGACTAAACCACCAGGGTATATTGTAGACTACTTTCCCCACACTTTGCTGTGGCCATA GTTATTATAGAAGCACCTAAGTGATCGCTGTAATCAAAACCAACCCACATTATTAATGAGAAGCTAAAAC TTGATTTCTGTAGCAAAGATAAAATCCAAACATCATCCTAGTGAAGATATATACACATAAGCTTTGAGAG CTGCTGAGCACTGTAATATACAAAGTTGACATTAGTGAATACTGCCGACACTGAAAGACTGACCATCCTC CTAAGCTAGGGCCCTGCCTACCCTAGACAACACAGGAAGAGGTTCCGATTTCCTGAAGCTACTTAGTGAA GCTCTCCTTGAGGATGCTAAGAAGCAACCACCTTGGAGACCGAAGGTTTAGGGTATCCCAAACTCTTGAT GTTATAAGTTTCCAGAATGAACCTGTTGTGAGATGTCCTTTGATGGTTTATTTGCTAAGAGGGAATTTTG GTATTTTATGCATGGTTCATTGTTGCCAAGAAATACATAAGAGAAAAGGCATCTTTACATACAGGTTTCT TTGGAAGAGACCACAGACTGAAAAGAGAAACTGATTTTTTTCATACTGCCACGTTTTTTAAAAATGCTAG GAAAAGCTCTACTGAGAGTAAGTTATATACTGTGACCACAGCAAAGTGAGTCTTCGACTGTATGGCGCCT GTGCTCACTTACATCAGAGCTATTTATTCACTGCTGCCTTTAAAACTATGTCTTCACTGAAAACGTGACA GGGTTGTAGTGCCTTCTTAGCATATGAAGTTGTGATTTTAAGACAGTAGAGTGCCTTTCTGCTGTAGCAC ACAGCATAACTGTTTCAGTGTCCCGCTGGTACCTGTTCTTATTCAATAAATCTCTCAAAAGACCAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30121331 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30121331 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17603 same embryo
	EMAGE:17605 same embryo
	EMAGE:17604 same embryo
	EurExpress:euxassay_005940 same experiment
	MGI:4827044 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source