EMAGE mouse gene expression spatial database

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EMAGE:17617

Cbln3 cerebellin 3 precursor protein ( MGI:1889286)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006050_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17617_wholemount_moderate.wlz

17617_wholemount_weak.wlz

17617_wholemount_possible.wlz

17617_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17617_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9506
Entity Detected:	Cbln3, cerebellin 3 precursor protein ( MGI:1889286)
Sequence:	sense strand is shown >T9506 AGCAGAGGGGGGAGAGCCAAGGGGACAGAAGACAGGAAAATCTGTGAGTTACAGAGGCACCTAGCAAGGA TGGGGACGGAATGGCACAAACCCAAGCTGTCCTTGGCCCTGGTGCTTCTGACCCTGGAGGCTGGGTGGGC TCAAGAAGGGTCAGAGCCTGTCCTACTGGAGGGGGAGTGCCTGGTGGTCTGTGAGCCAGGCCGACCTACT GCAGGAGGACCTGGGGGTGCAGCCCTGGGAGAGGCACCCCCAGGACGAGTGGCGTTTGCTGCAGTCCGAA GCCATCACCATGAACCAGCAGGAGAAACCGGCAACGGTACCAGTGGTGCCATCTACTTCGACCAGGTACT GGTCAATGAGGGCGAGGGCTTTGATCGGACCTCGGGCTGCTTCGTGGCCCCTGTCCGTGGAGTCTACAGC TTCCGATTCCACGTGGTCAAGGTGTACAACCGCCAAACTGTCCAGGTGAGTCTGATGCTGAACACATGGC CGGTCATCTCAGCCTTTGCAAATGACCCTGATGTGACCCGAGAGGCAGCCACAAGCTCTGTGCTGCTTCC CCTGGACCCGGGGGACCGTGTGTCCCTGCGCCTGCGTCGGGGGAATCTTCTGGGTGGCTGGAAGTATTCG AGCTTCTCAGGCTTCCTCATCTTCCCACTCTGAGGACCCAAGCCTTTAAACGGAGATGTCTAGCATCTTT CTGCCCTTCCCTGCCCCAGAAACAAAGTATCTTTGGAGCAAGAGAAAGGCCCCCTCCAGGCTGCCTACGG CCCACCTCCTTGCATGAGATCATAGTTAAACACCCAAGGCTAGGAGCAGAGCAGAGCTACGGAACCTCTA GACAAAACGCCTCACTCAGGTCGCCCTCCCAGTCTCCTGCTGCCCCTCCTGCCCCTCCGCAGCTCCAGTT TAAGGCAGGGTTTGCTAAGAGGGAAGCTTGCACTTCTTCACAGACTCCACTCCTCCTGTTCTCCACCCCA GCTTCCTGCTCAGCGCTATTTGGTGACAGGTGGCGCAGGTAAGCTTGACAGGCTCCCACAGGGGCCCAGA TGGACCAGCCTCATAGTACCTGCGGGCTCCATCCTGTGAGGAATGCCAGCAATGAGAGCTCTCGGCCAGC ACCCTGTGAACAGGGCTGACAGCTCAACCACAAAAAAGCAGTGTGGGGAAGGCCACAGAGCATCCGTAGC TGGAGAGACAGGATGGGGCCATGTGCCTACCCTGAACATGTAAACATTCTGCAGGTAAACTGGGACAGTC AGAAGGCAGGAATCCTGCCGTCCATCTGTGCCAGGTCCCAATGGACTCTGGCCCTCATTGCCTGACCAGA GGCTTGTGTGTGTGTGTGTGTGTGTGTTTGCTCTGGCTAATAGGGGAAACTAGAGCAGGTATACAGACCT GGAGGCAGAGTCAAAGGTCACGAATGAGTCATCAGTAACCACATGTCCTCTCCCTCAGCCACCTCCTAGA ACTGCCCCACCTTGGAAATCTGAATTTCAGTCACTCCCTACTCTTGATCGACATGTAGTTCCTCCAGCTG TCTCCCAGCTAATTTCCCTGTAGTCTGCTCCCCCCCCCAAACGCATCGTTCTTCCTCTACTTCTACTACT GTCTTACTCTTCTTTTAAGGTCTCCTGTTACCTGGGAGTCCATGACTCATCTCCCCCAACTAGTATCCCA CCAACATCAGAAACTCTTTTTACCCAGTTGTCCCAATGGTCCAGCATGAACGGACCTACCTAGAACAACT GGGGAGCTGCGGCTGTTAAAACAGTGACTTGTCCTGTTCGTGAGGGTGTGAAACAGGGACTGCAGGACTA CAGCATGCTGAAGAGAAATAAATTCAAAGCTGGGCATAGCAGTCAACGTTTTTAAAGTGCTATAATATAA AGTACTCGTGCTAAATAAATACCAGGGCAAGTTTGTGGTTTTTTGTTTTGGTTTGGTTTTTGGTTTTTTG AGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAAGCTGGCCTCGAACTC AGAAATCCGCCTGCCTCTGCCTCCTGAGTGCTGGGATTAAAGGCGTGTGCCACCACACCCGGCAAGTTTA TGGTTTTAAAATAAAAATCTAGGTGTTCATTCAGGAGACAGAGGCAGGTGGATCTCTGTGAGTTTCAGGC AAGCCATAGTAAAACCTGTCCAAAAGAAAGACAGAGAGACAGAGACAGAACAAAGAAAGAAATAAGGATG GAGGTGTCACTCAGAGGCAGACGCTAATACGCATGAAGCACTGGGTTTGAACCCCAGCACTGGGGTAGGC GGGTAGGGAGTAGACAAGAGGGGGAGGGGCAGCACTTTCCTTCAGCATTTGTCCTTACTTTGTGGCCAGG AAGAATAGTTTAAACAAGATAAGATAATATTTCTGTCTCACATGGAAGAGTCTGCAGCTGGGGAGTTCAG GATAAACAGCTGCTTAGAACCACAGAGCCCAGTCTTCCCTTTTGGGATGCCTCTCTGGCATGCCGCCTCA TGGCCCAAGATGGCTTCGTGCAGGTCAGCATTACATCCCAGCAGCGCTGGAAAAAGGGAGAGAAGAAAGA ACACAGCTCCTTCCTTGAAAGGTTTTCCGACCGCTGACAGCTGCCTACACACATTTCTGATTGCATCTCA TCGCCAGAATGGAGTCACACTTGGCCACACCAAGCCAGGACAGGCAAGGAAACAATCTCACAATCTTACC TTTTTGTCATATTTTCCATGTGCATGCACCTGGGACGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGA GAGAGAGAGAGAGAGGGGGGACAACTTTGGGTGTTGGACCCCACCTCCCACTATGTTTGAGACAGGGACT CTAATTTTTCATTGCGTACACCAGGCTAGCTGGAACCTTCTGGAATTCTGCCTTCTCCTTAAAGTACAGG ATTACAAATGCATGGACTATGGCTGGCTGGAATATGAACTCAGGCCAACAGGTGTGCACAGCAAGTGTTC TAACCACTGGACCACCTCCAAAGCCCGTAGACTTTATTCCAGGCCATCACATCCAAAATCAGGATCTTAT AGTAAGGAAGAAGAGTAATTGGACATTGGAAGTTTTTCAGAATACAATAAACTAGATCAGCCCGTTAACA CCGGGAGGTACACGATGCTCTTTATTTATTTATTGCACCTTCTATATGTCAACAGTCTGGAAAAGCGTGA CTATTCTCTGATAAAGTTTCACAACGCTGAAAATAAGGTGGCAGGTGGTACCCTTCCTTCCCAGAAGGAA AGAGAGAAGAAGACACTTCCAAATTCATTTAGTGCTCAATCCCTTAGAGCTGTAGTGCTGATATCCTCAG GAGCTTCTGTTTTCCAGCTAAAACACACACAGCACAGCCCCGCCCTCTGACTGGCTCCAGCCTAAACCTG CCACATCTTTCTGATGTCCATAGGAAAAGTTCCTGTAGGTTGAAAATTCGATGCTTAGACTGGACCCACC GAGAATATACAGAGCAATCTTTCTATTTTAAGGTCCCCACCAGCAGTGTATTTGCAAAGTCCCCATGCCA TGTAAACGCATATTGAGATATCTTGGTGATTAGAGTATAGACTCTGAGATATCTGCATAGCACAGACTTA AAATCTCAAGATGATGTTTTGAGAAAACACTTGGCACAATTCACCCACTGAGATACTCAAGACAAAGAAG ATCAACATAGGCTTTGGTTTTGGTTTGTTTTACTTTTTAGTTTATTTTTATTTTATAGTGTTTTGCCTGT GCACCACATACATGCAGTTCCCTTGGAGGCCAGAGGGGGTGCCTCAGATCCCTTGAGACTGAACTGCAGT GGCAGTGAGCCACCTTATGGATGCTGAGAGTGTAAGCTGGATCCTCTACAAGAGCAGCCAGAGCTCTTAT CCATTACTCCATCTCTCCAGCTCGCTCCTCCACTCACACACACTCTGGTTTTAATCATTTTAGAAGGTAA ATCAGTTCCTACCCAAACAAGAATTCATTGGTTGATAGTCCAAGACTTCCTTGCCATGAAGAAAATGCCC CTCTTTTCTTTTGACCCAGAAACAGATGTCTTGTCTTTAGGCCACATAGCCGTGTGGCCCTCTTTTTTAC ATTTGAAGACCAAATCCAAACTTGGTCTGAGTGAGATAGTATCCACTTCATAACTACAAATTCATTTGTA TGATAGTGAGAGTGGAGGTTTAAAATTGGACAATGGATGTCCCATACCTTTAATCCCAGCCCTCGGGAGA CAGAGGCAGGTGGTTATCTGTGAGTTCAAGGCTAGCCTGCTCTGTAGAGTGAGTTCCAGGATAGCCAGGG CTTGTCTTGACACAAAATAAAACTGAGCAATCCAGCCTAGTGTGATGGTACACACCTGTAAACTTGGCAC TTAAAAGACAGAAGCAGGAAGATCTGGAGTTGAAGGCTAGCCTGGGCTACATAAACTCTGTCACACATAC ACACACAAAACTTGTATAATGCTGTAGTGATGTGTTTGCTTTATCAAAATGCTCAAAGCTTTTAAAAGTG CTGACCTATTAGACTTGCAATTCCATTTAAATGTTCAGGAGAATTTAATTGAATTTGTAATCGGTGTTTA AGTGATTTGACTTGTAAAATTTGTTGTAAAAATTGTTGATTTGTTGGCTATGTGCTTTTGTGTTCCATTT TGCTTAAGTAGGTTTCTATTAAATACTGAAGAAAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30536445 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30536445 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17618 same embryo
	EMAGE:17619 same embryo
	EMAGE:17621 same embryo
	EMAGE:17620 same embryo
	EurExpress:euxassay_006050 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source