EMAGE mouse gene expression spatial database

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EMAGE:17620

Psd4 pleckstrin and Sec7 domain containing 4 ( MGI:2674093)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006060. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006060_01	euxassay_006060_02	euxassay_006060_03	euxassay_006060_04

euxassay_006060_05	euxassay_006060_06	euxassay_006060_07	euxassay_006060_08	euxassay_006060_09

euxassay_006060_10	euxassay_006060_11	euxassay_006060_12	euxassay_006060_13	euxassay_006060_14

euxassay_006060_15	euxassay_006060_16	euxassay_006060_17	euxassay_006060_18	euxassay_006060_19

euxassay_006060_20	euxassay_006060_21	euxassay_006060_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17620_wholemount_strong.wlz

17620_wholemount_moderate.wlz

17620_wholemount_weak.wlz

17620_wholemount_possible.wlz

17620_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17620_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 12 13 14 15 16

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

telencephalon mantle layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon marginal layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

olfactory cortex mantle layer

strong

homogeneous

strong expression: see section 10 11 12 15 16 17

olfactory cortex marginal layer

strong

homogeneous

strong expression: see section 10 11 12 15 16 17

hindbrain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

midbrain mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15

not examined

regional

not examined expression: see section 15

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07

spinal cord mantle layer

strong

single cell

strong expression: see section 04 05 06 07 08 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 12 13 moderate expression: see section 04 14

not examined

regional

not examined expression: see section 01 02 03 21 22

neural retina

strong

spotted

strong expression: see section 01 02 03 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9507
Entity Detected:	Psd4, pleckstrin and Sec7 domain containing 4 ( MGI:2674093)
Sequence:	sense strand is shown >T9507 GGCAAAGCAGGCCCAGAACAGAGAATCCTTAAGGCTCTCCACACCCAGAGGCATTCATGAGTCCAGGCTA GATATGGATTTCCACCTTGGCTTGTGCACCTACTAATTTGTGAGATGGGATTGTGAAAACAGATGCTACC AAGGGCTTCTGCTCAACCATCACTCTGTGGATGATGGGGGACCACCTCAGACCCATGGAATGTCTCAATA TTTGCTTGGAAGACAACTTACAACCCTGTCCAGAAGCATATCCAAGTGAAATACATGGCCATCCAGGCCC TTCTGAACCTTGCCAGGAACATACCTGTCCGTTTGACCCCCCAGAATCTGCCAGACCTGATGCTCCTCAT GGTAACTCTGGTGTGGAGTCCACTCATCTAGAAAACTGCTTGGTGCAGGTACAGGCTAGGCAGGCCTCAG CATCACTGAGGTCACTGGAGGACAACAGCTCTTTGGGGAGTCCACAGCAAAACCAGAGCTCATCCACCCA GGTGGTATTCTGGGCTGGCATCCTGCAGGCCCAGATGTGTGTCCTGGACCTGGAGGAGGAGCTGGAGAAA ACAGAGGGACTCAGAGCAGAACTCAGGTGCTGCATCCCACCACCCTCTAAGGACCTCCTTGGAGATGAAG GTCTGAGCCCCAGCCGACCCGAGGAGGATGAAGACTCTGGAGATGACAGCAGTGGGCCTGAGGAGGAGAA CCAGACCTGGCCAAGGGAGAAAATTCCAGGCTCCTCCCTAGAGTGGGGTGCTGAGGAGGACAGTATCTTC TTTGATAATCCCCTCTTCCTGGAGAGCCCTTGCTCAGACACCAGCACTGAAGGAGAATGCTTTTCCTGGG GGTACCCGAATTCCCACCCAGACATGAAGACCTGGCATCAGAGCCCTCAAACTCTGGACTCTCCTCTCCA AAAAGGCACAGGGCTCTGGAGGCAAGAAAATGAGCTGGATTTGGGGAGCGACACTGCTGACCATGGTGGG TGTTCCACCCCTCCATTCCCTGTGCCTTCCTATAAGATGCACCCCTGCTTGGCCTTAGGATCCACTGAAG GGGATCCCACGGTACCTCCTGATCAGGAGGGTGAGACTTCTTGTGAAGATGATCTTGGCCATGGCTCACC TAAAGCACCCTTTGTAGACCATGAATTGATCCAAGAATCAGATAACTTTGAATTTGACCTTAGACCTGCC ACAACACATCCTGTACAGCCTTGGGGTTCACAGACATCCCAAAGCCTTTCTGACCTGACTCAACCCATAC TTGAGGACCTGCAGAGAGAAGATCCTTCCAGGTCCCAGGAAACTCTTATCTCCCAGAACAGAGGTGAGAG GGATGCTGGCTGTTTCCAGGAGCCCGTGTTCTGCACTTTGGCCCCCTGGGGGAGCCAAACCTCTTTACTG GAGCCTAACTGCCCAGAATCTGAGGGTAGAGGCTCTGGCCCCCAGCCCAGCCCAGTGTCTTCCCAGGATA GCAGCCCACGGGTTCTGCTGCACAGCCCCAAGTGGCCACAAGATGCCTCACACCTTCTCCAGAAGGATAG ATCAGAGTTGAGCTCCCTAAAGGAAGAGGAGACAGAGGAGGTCCCAAGCCTGAGGCAGGAGGCAGAATGT GAAGATACATCCAGAAGTGAAGATGCTTCAGCCAACCAGCACCATGTTCACTTGGCTTCTGCAGAAGGGC TTCCTGAGAGCCCCATGCCCCAAGCTCAATCTCCAGAGGAAGGCTGGAGGCCATCATCGTCTAGAGAGAA GTTGGCTAATGACATCAGAAATGACAAGGGAGCATGGAACCTAGCCCTGCGCCTCTATCAACTGAATGGT TTTCGGAAGTCTGAAGTAGCTGCCCACCTACGCAAGAACAATGACTTCAGCAGGGCTGTAGCCGAGGCCT ACTTGTCTTTCTTCCAGTTCGAAGGTCAGAGCCTGGACAGAGCCCTCCGTGGTTTCCTCCAGGCCCTGGT GCTCAGTGGAGAGACTCAGGAGCGGGAACGAATTCTCTACCAGTTTTCCAAACGTTTCCATTACTGCAAT CCTGGAGCCTTCCCCTCAGTAGATTCTGTTCACACGTTGACCTGTGCAATCATGCTCCTTAACACAGACC TACATGGGCAGAACATTGGCAAGAGCATGAGCTGTCAGGAATTTGTAACCAACTTGAATGGCCTGCAGGA TGGCAGGAACTTCCCCAAAGAGCTGCTGAAGGCCCTCTACTGGTCTATCCGCAGTGAGAAGCTCGAATGG GCTATAGATGAAGAAGATGCAGACAGACCCGAGAAGGACCAACCCTCTCCCTCAGCTGGCAAGATAAGCA GCCCCTTCCTGCAGATGGCTCAGGATCCCACGATGCCCACCTATAAGCAGGGCATTCTGGCTCGGAAGAT GCATCACATTGCTGATGGCAAGAAGACTCCATGGGGCAAACGAGGCTGGAAGATGTTTCACACCTTACTG AGAGGGATGGTCCTCTACTTCCTGAAGGGAGAGGGCCAATGGCTGGATGGAGAGAGTTTGGTAGGACATA TGGTGGATGAGCCTGTGGGAGTACACCACTCACTAGCCAGCCCAGCCACCCACTACACCAAGAAGCCACA TGTCTTCCAGCTTCGGACTGCAGACTGGCGTCTCTACCTCTTCCAGGCACCTACTGCCAAAGAAATGGCT TCCTGGATTGCGCGAATCAACCTGGCGGCTGCCACCCACTCAGCACCTCCTTTTCCGGCTGCAGTGGGCT CCCAACGCAGATTCGTGAGGCCCATCCTGCCCATGAGCCCGGTGCAGAGCTCCCTGGAGGAGCAGCATCG ATCCCACGAGAACTGCCTGGATGCTGCCTCAGATGACCTGTTGGATTTGCAGAGGAACCTGCCGGAGCGA CGGGGCCGCAGCCGCGAGCTGGAGGAATACCGCTTACGGAAGGAGTATCTGGAGCACGAGAAAACTCGAT ATGAGACCTACGTACAGCTTCTGGTGGCCCGCCTACACTTCCCTTTGGGGGACCTGGCTCTGTGGGAAGA TCAGCTTGGGAAGGAAACTGACGGTTCTCAGGAGCCCAGGCCAAGCCTGAAGAAGTCCCACTCAAGCCCA TCACTGCACCAGGAGGAGGCCCCCACCACAGCCAAGGTGAAACGCAACATCTCAGAGCGCAGAACCTACC GGAAGATCATTCCCAAGAGGAACCGCAATCAGCTGTGAAGCTGTTACCCTCTCAGACACTGACCACTGCT CCGGGGTGGACCTGAGATGCAGCCTTATGTGCCAGGACCATGGTGAAGGATAAGGCATCTCTGATTCTTT GCTGAAGGACGGGGCTGATTTCTCTGTGTCCCCGTTCCCTGGCTCTTTAGAACTGCCTTAATCTTGCCAT ACTTGCTTCTGTCCCCCATGGTGGTTCCTCCATCACCCTAACCCTGATCACTCAAGAGCAAGGTTGAGTG AGAAATTGGCTCCACTTGCGGAATTTGTCCCCCATGCACTCTCTCCTCTGAAACACGGGGCCTGGGTTGC TGGGAGGTCGCTTGAGGAAGTCTAGCTAGGACTGTTCACTGGGAACAGTCTTACTTTGGGAATTAGGTGA CCTAGCAGGAGATGGTCCCAGAAGCATAGGGAAGGACCTCTTGGGAATTCCTAAGCACCATCTTGTAAGC AGGGTTACTCTGGCACTGCCCAGTAGAGGACAGCTTCTAACAAGAGTCCTTGGTAAGTTCAGGACTCAGG GATGCTGTGGGGCTCCAAGCTTCAGATCTTCTGACTGCTTTATCCTACAACCAGCCAAACAAAGCCCTTC CCGGGACAGGGAGTACATGAAAAGGCTGCGTGCCTAATTGTTGTTAATCAACAGAGCAGAGCTTGTGGCC TGAGCCAGGGTCAATAGGAGTGGGGGCCTCTAGAGCTCTATGGAGAACCCTTGGAACAAGGAGCCTTTGT TTCCTGAGAGTAATCAGGGTAGCTTTTCTAGGGGGTGAAGGTGGTGACAGTTCTCCTTGGGAGTCTGCCT GGCAGTGGGAGTGGAGATTGGAGGCCTGGGTTCTCACTGCTTTCTGTAGTCACCCCCATCCCACCTCCAC CTTCAATATCCCTCAAAGCTGCAGTCCTGAGGGTTGACAGGACCTATTTCTCATTCCTGGGGCAGCTCCC AGGCTCTTGCTGCCCTGCCTTGGGAAATCCCCTTGCACTTCTTTTTGGCTTCTTCCCAGGTATGCCTGTT CTCCAGGGGAGGTTCTGGAGACTCGGAGACTTAGGGCTGTGTCTGTGAGGAGGAGGCTGGCCTGAGGGAG CAGCCACATTGTCTCTGCTCAGCCATGTGTGCAAAGAGGTTGCTGGCCAAATGGAAGCCTCTGCTGCTCA CCCTTCAGCTGGCACATTGCCTCTCCTGCCTTCCTGCTGGGCCACACATCCTCCCTTCCAATAATGCTTA GAGCACCACAAAGCGGAGCTGGTCCTTAGGGCCAGGGGACTCTCTGGCCAGGGCCTGGGGCTGGCCTTTG CACCCAATGGGTGTGCAATAAATAGAACCTACCACAGAAGTGGGTGAGTGTGAGTGTGTGTGTGTGTAAG GGGGAGTCAGTCAGGTAGCCGTGGCCACCTGCAGTGTGTCAGGCTATAGCTTCCTTTGCCCCCTTTGTGA TAGTATGTCTGTGTCTGTGTCCTGCATGTTCATGTGTGTACCCCAACATACAATTACAAGCTAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30361859 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30361859 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17618 same embryo
	EMAGE:17619 same embryo
	EMAGE:17621 same embryo
	EMAGE:17617 same embryo
	EurExpress:euxassay_006060 same experiment
	MGI:4827466 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source