EMAGE mouse gene expression spatial database

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EMAGE:17621

Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) ( MGI:1919352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005951. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005951_01	euxassay_005951_02	euxassay_005951_03	euxassay_005951_04

euxassay_005951_05	euxassay_005951_06	euxassay_005951_07	euxassay_005951_08	euxassay_005951_09

euxassay_005951_10	euxassay_005951_11	euxassay_005951_12	euxassay_005951_13	euxassay_005951_14

euxassay_005951_15	euxassay_005951_16	euxassay_005951_17	euxassay_005951_18	euxassay_005951_19

euxassay_005951_20	euxassay_005951_21	euxassay_005951_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17621_wholemount_strong.wlz

17621_wholemount_moderate.wlz

17621_wholemount_weak.wlz

17621_wholemount_possible.wlz

17621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9515
Entity Detected:	Dusp11, dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) ( MGI:1919352)
Sequence:	sense strand is shown >T9515 CCGCGGCCTCTTCTGGCCTCCCTGAAGTCGCCACTTTGGCCGCTCTGGCACGCCTGGCGTTGGGTGGGCG GCATCTCTGCGCCCACATGAACCAGCATTATGGCCGACATGGTCGTGGACGGGGCCGGGACTTTGCTGCC TGTGCCCCACCCAAGAAGAAGGGCAGAAACCACATCCCCGAAAGGTGGAAAGACTATCTCCCAGTTGGGC AGCGGATGCCTGGGACTCGTTTCATTGCTTTCAAAGTTCCTTTGCAAAAAAAATTTGAAGCAAAGCTTAT GCCAGAAGAATGCTTTTCCCCTTTGGATCTGTTTAATAAAATACAAGAACAAAATGAAGAACTTGGATTG ATAATCGATTTAACATATACTCAGCGCTATTATAAAGTAGAGGATTTGCCAGAAACTATTTCTTACATAA AAATATTTACAGTTGGTCATCAAATACCTGATAATGACACTATCTTTCAATTCAAATGTGCAGTTAAAGA ATTTTTAAAGAAAAATAAAAACAATGACAAACTTATCGGTGTCCACTGCACCCATGGCTTAAATAGGACT GGCTACCTCATTTGCAGATATTTGATTGATGTAGAAGGCATGAGGCCAGATGATGCAATTGAATTGTTCA ACAGCTGCCGGGGGCACTGCATAGAAAGGCAGAACTACATTGAAAACCTGCAGAAACGTCATGTCAGAAA GAACCGGAATGTTAGTGCACCTAGGACAGATGGCCTTGAAGACTCAGCAGATCCTACGGAGCAAGTCTAC ACCAACAACAAACCTGTGAAAAAAAAGCCTAGGAAAAACCGGCGTGGTGGTCATCTGGCACCTTCCCAGC ATTTCCAGCACCAGACGCAGAGCTCACCGTACTCACTCAGAAAATGGTCACAAAATCAGAGTGTTTACCA GAGAGGCCTTGTCCCTCCTCCTGGTCCAGCCGGAGAGGACTATTCACAAAGAAGATTCTTCTGGAGTGCG AGGCCAAATAAGTGGACTGCTGAGAGTTACCAGAGACCTTTCTACCCATACTACTGGGAATGGAACCTGT GAGGCACGCTGTGGCTGGACTGTCGTCAGGGCCACCTGAGACCAGAGCTGGACAGAAGACAGCTGAATGA ATTTAATAAAATCAGGTCAAAAGAATTTAGAAAACTTTCTGAACATTTTTAACTGTTCAAACTGTTGTAC TGTCCTGATATATGTTACACTGCTCTGTGCTGGCGGACGCGGTGGCTCCGGCAGTCTAGAGCACCTCCAT TCCAGGCCTCAACTTTTCTCAGAGTTCTGAATAGATAACAAATTCTAAATTGCCTTCTGTTTTCCCTGTA TATTTCCTTTGCTCATGCTTGTAATGTGTTGTGTAGGTGCAGAGATTCGTAGTGTTTTTACCACATGTTC TTTACTGACAATCTTTATGGGTGTGTTTTCCATGACTGTTCAAATTTTCTACTAAAACTCTAGCCAGGGG ACAGTTCTTTTCTTTGCACCTGTTCTTTGTCCTCGTGGGACTCTGATCAGCGTTTTTTCCTTGGTGGTCA GTGTTGGGATTTGACCAGCTTCTTGCTGACCTGCCAGCTAAGGCATCTAACACAGAGAAGGTTCAAACTT TAGGTACTGGTTGATTGCCAAGTTGGGTAACTGCCACCAACAGGGTAGAACTATCCAGGAGGCTAGAAAC CCTCTGTAGATTTTAAAGGTTGAACAGACATCCTTGGATATCTGGGAAAGCTGAGGGTTATACTGACTCC GAGGACCTCAATTCAGGATCTCTGTCCACTGGCCTCCCACACTAGAATGGTGCTCTTTCTCTAGGACACA CTTCAGGCTACATGGAGGTGCTAATTACTTGTCCTTAGAACTCACTCAGGAAGTTGCTCCTCATAACACA TCAAGTCCACAGCAAGTTTTTGCTATGCACAGTAGTTTTCTCTCTCATGTATGAACATGTGGAGGGAACT TTTCCCTAATGAAGTCCCGGCAGATCATGTATTCCAGCTTGCAGCTAATTTTGCCCAAACTTTAGTCAAG TTGCTCTGCACTCCAGTGATGGCTTTTTCTCTACCCATCACATTAACAGATTCAAAGTGCTTCTTGTTGT GAGACCCCCCCACTCCACCCCCGAGGAATTCTTCCCCAGAATATACTGGGTCAGTGTATTGTCCAGTGTA AGAAACTGGAACAGACAGTTAGGAACCAAAAGAGGACAAAGCTGGGACAGGTACAGAATGCACCCAGTCT GGACGGTGCAAAGAACACATTGACAAGCAGCCTCTGGAGGACACTGCCAGGTAGGGTCACTGCGTGAGTT CTGCCACGGTTAGGAGTTGTTCCTGCCACCTGCCCTGTTCCTCTACCAGGAATTGGATTGCCAACACCTA GTGAAAACCACCTTTATCATTGGATAACTCATAACTAACTTTCATTCTTACACACTCGTCTGGCTTCTGT CCATGTCACACAATACAAAGGCCTAGTGGAAGGACACTTCTTTTTTTAAAGTTTCTAGTGGTCTATTTAG TATTCTTAAGTTATATTTTACTTTAGAAACATACATGAATATTTTCTCAGGTAGCTTTCCTGAAATTCCC ATGGAAAGGCACCCTGCATGTTTCTTGATTGTCAGTCTCAGAGGTAGCCCGCCTAAGGATTTCTTATGCT ATGTATTTTCCTCCTTTTTTTTTTTTTAAATACTCTTACAGATCACCATTGCTTCATTTTCCAATTTATG ATAACATGCGTTGATGAATTCTTCCTTTGGCAAATGAGGATTTAGCTCTCAGCCTACCCACTCACTCCAC AGCCCTTTGCAGCCTTTGATGAAGTTACTAGATAAGTGTGGTCATCACGCGCGTCGTGTTCATGGCTGTG GGTTCTTTCTGGCCTTCCTCTTTCCTTAACGTTTTTGCCTCATTCATTTGGCCATTTTCTGTTGATTATG TCACAAATTCATCTTCTCCATCTGATAGCATTATAAACCGTCTCAGTGAATGATGCGCCCTTTGATTTGA CTCCTTTTCTGGAGCTTCTTTAAATGCCCCAGTCAGGTGCCAGGTCTCGTTGGTAGGGCTGCCATGCTAG AGTACCACAGACTGAGGGAGAACATCTATAGGAACTTTTTGTGTCTCATAGTTTTAGAAGCTGGGAAGGA TAGAGTCAGCAAGAATTTCTTCTTGTAGATGACTGGTACTTCACTGTCTTCTTGCGGTCTTCTGTGTCTT AACATCTCCTCCACTCGTCCCTCCCCCCCTTTCCTTTTTTCCCTTATGATACGCTGAGATGCATCGCCAG CTCTGTCTTCTCAAAGATGCCAGGTCATTAGACTCAGACTCACTGTGGAGACCTCATTTGCCTCTTGAAA GACTCCCCAAATGCGGTGATATTTCAGATACTGGGGCTTAGAACTTTACCAGCAATTTGAGGAAACACAA CTGTCCATAGGAGGCAAACTGGCTGTTCCTTACACGTGGGGCAAAGCTCCCATCTGAGCTCTCTTCCCTC TTGTCATTTATTTCTTAGAACCTGGGTCCCAGGGTTCTACTTCCATGACTATAATTTCCCATTTGCTCAT TATGTCCTTCAGCAGCCTCTCAGGGCTGGGAGGGTGTAACAGCCGAGCCACAGAAGTGGATCTGTTTGCT TCTCTGCTCCTGTCCTTTGATGAGCAGTAGGTAGGCACTTTTCAGAGTCCTAGTTCATGGTGTTCAGACG GTTGCTGTTGGCTGTTGAAACCCTGTCTCCTAATCTCAGATCTTTGGGACCTGTAATTCTTCTCTTGAAG TCTTAGGGTCTTTATCTCACATGTCATAGTGTATTTTCTATCCACCATGACATATACCAGCTGAGTCCTT TCAATATGGCAAATTGTAAGAAGTTTTTATTTAATGCTATTATATTAGCATTAATCCCTAACCCTAGTGC AGCTTCTAGTAGGATGTTGGACTTCTTTCCTGAAATTACATCTGATTTAATTATCTGTTTTTATTTTTAT TTAACCTTTTCGTGTTACTATCTAGCACTTTTCTTCTGATACATTGGGGTTGTAGATGCTGGGATTGACC AGGGCCTTGAGCATGCCAGGCATGTACTCTGTAATTGAGCTGTGCCTCCAGCCTTCTTCAGACCTATATA TGTCATTTGTATAAACATAATAAAAATACAAAAGATAAAGCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30364073 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30364073 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17618 same embryo
	EMAGE:17619 same embryo
	EMAGE:17617 same embryo
	EMAGE:17620 same embryo
	EurExpress:euxassay_005951 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source