EMAGE mouse gene expression spatial database

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EMAGE:17639

A430105I19Rik RIKEN cDNA A430105I19 gene ( MGI:2685199)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005980. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005980_01	euxassay_005980_02	euxassay_005980_03	euxassay_005980_04

euxassay_005980_05	euxassay_005980_06	euxassay_005980_07	euxassay_005980_08	euxassay_005980_09

euxassay_005980_10	euxassay_005980_11	euxassay_005980_12	euxassay_005980_13	euxassay_005980_14

euxassay_005980_15	euxassay_005980_16	euxassay_005980_17	euxassay_005980_18	euxassay_005980_19

euxassay_005980_20	euxassay_005980_21	euxassay_005980_22	euxassay_005980_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17639_wholemount_strong.wlz

17639_wholemount_moderate.wlz

17639_wholemount_weak.wlz

17639_wholemount_possible.wlz

17639_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17639_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9541
Entity Detected:	A430105I19Rik, RIKEN cDNA A430105I19 gene ( MGI:2685199)
Sequence:	sense strand is shown >T9541 GGCCTTACTACGAAGGTACCAGGAGATCGAGGAAGATCGTCGGCAGGCGGAGCAGGGGGGCATGGCTGTG ACCACTCCAGGGCTCCTCCAACCCGACAGCCTCACGGTCACCATCAGCCAGGTTCCCGGTGAGAAGAGGG TGGTCAGCAGAAACTGGGCCAGGGCGCCCCTTTCACCTGGAGTGACTACCACACTGACAGATGATGAAGA TGCTGCAGACGCACCGGGTACTTTCAGCATGGGGCACCGGGTGGAACTTGCTGTCACCATGGAAAACAAA GCCAAGGCCAAAAGGATAGTAAGTGAGAAACCCACGACCCAAGCGCGGAGCCCAAGGGCAAAGGGGACAT CTGGAGGAGGCCGGAGCTGGCGTGCACCCTTGCAGATGACTTCCAGCTCAGATTCAGCAGGGAAGGGTGT TCTGGAGCCCCGGAGTCCAGGCGTGGTCTCAAGTCCACCTCCCCAACAGTCCTTGGGGCTGTCCCCTGAG GCCAGCTGGGACTACACGCAGTGGAAACAAGAGAGAGAGCAGATTGACCTGGCCCGCCTTGCCCGGCACA GAAACCCACAGGGTGACTGGAGCCGCCCGTGGGACCTGGACAAAGCCAAGTCCATGCCACAGAACTGCCA CAAGCCTAGGGACAGGGACCTGGCCATGGGCAGCAGGAAGGGTCCTAGGACCCACCAGAAGCCTCAGCAC CCACCATCATCCCTGGATGGAAAATGTCGGGGTGGACAATCTGGAAGACCCTCGATGACATCCACCATAG GCAGCAACGCCCAGGGGAAGGAGAGGCTAACTGGCAGGGCCCGAAGGTATGACATGAAAGAAGATGAGAT GCTGCAGAGCCAGGAGGGAAGCCAGAGCAGCACGAAGACTCCCAGTGCAGAGGAAGAATTCGTACAGAAG CGGAATGAAAGGGAGCCAGACAGACAAGAGACCGTGCCTGCAACCAGCCCAATTCCAGCCTCCCCAGAGG GGCTAAAAGGAGAGTCGGGGACCTCTACAGCCAGTTTGGCCCCAGACTCTCCACAACATTCTGACTTGGC TCCCCTCGACCTCTCTGTGGGAGGAGCTAGCAGCCGCAAGCCTGGAAAAAGTACATGTATACTCGGTCCA AAGGCTGGGGCTCAGCAAAGTCCAGTGTCATGGCCAAATGGTTCTGAGCAGCAGACCCTGGGGTGCACTG ACCACCAGCCTGGGCTGGAAGTCCACAGTTGCACTGAGTCACAGAGGAAAGCACAGACCCCAGAGCCCAT GGAGGACAGGGCTGGCAAGGCTGGGGCCCAAGAGAATCTGACACCAAGAAGCAGGCCCCCCAGAGGAATT GGCCCAAGGGCAAGAGGCACAAGTAAAAGAAGCAGGGCAGGAGGTCCTGGCCCTGCAGGAAGATGCTGAG TAGAGCTCAGGAGAGTTGGGGAGCTAGGCTGCGAAGGGGAGAACTCAGTCAAGAGTCTTCTGTGTGGTGG GAGTTACGGCTGCTGGTGGGAGTCTGGTACCTTTCTGGTTGAGCAATGCAGCAAAATTCCACAGGGACAC CATGTAAGGGCCTCTTATGCATACCTCTACACTAGTGGGTTTCTACATGTAGTCAAGGCTCTTGGTGTAT TGGCCAGGCTGGGATCTCTTGTTCAAGGCTGGTATCCCTGTCCTGGTCTTACCTAGGCCATAGGCATTTC CCACCAAGCAAGATTTGGTAGGAGCCCAATACATGGATCTCCTGGCTCCTTATAGTTCGTCATTGCCAAC TCTCCCTCTCCAGTCACAAGCCTTCAGGCTCCTGAGCTCTAGGCAGTCTCAAAGCTGGCATGGCCAGCCT AGCCCCTTTCTGATAACCATGCCTTTCATCCAGGTGACTCACAGTTCTGCTCTTCTAAGATGGCCAAACC AGGATTTCAACAGTCGCTTGAGGCCTAGAGAAAGTGGTTTGGGGCTCATGGCTGACAGGAACACACATGG AGAGGAACAGAAAAACCCAAGGCTTCTTGGGGGGACAGGGCATCCAGCAGGGCTCTTTGAACTGTGCTAG GATTCTCCTTTTGCCACGGGAAAATGAGAGAGCTCACTAGTAGGTGCTGAGCCTTCCTCCGCATATCCAG ATCAGACATCGTGGGTGCCCTGAATAGACCCTGCAGCCCAGGGCCATTTCCTGCCCTCTCTTCCTTCTCC ACTTTCCCTCACCAAGGCCATGCAAAAGCAAGCACACAGTACAGTCAGAGGATTAGCAACCTCAGCTAAG TGCATCAGGATGTGTCTTTCCAACTCCATCCAGGCCCCTCCCATGAGGCCCACGTCTGGTGAATTATCCA GACTCTGCACAAGCTGTGGCTTCCTTTTGATACAACAAACATTTCCTGAGCATCTTCTCCCAGTAATCCA GCCAGTGGGCGAGGGTGACTCTGCCAGCCGGAGGGAGGGAAGACACTCAGTCATCCAACAGCTCAAGGCT GAATGGCAAGTGCTGTTCTCATCTGTAGAAGCCTGGTAGCCCCGGGTGTGATGAGGGTAGGTGCCTGAAG AGACCCACCCTGCCTCTGCTCCCCTCTTTTGATATGGCATGTGCCCTTTGCATCTTGGTGGCACCATGCA GCCCCCTCCTCCCTTCCTCTCTACAAGGGTAACTGGGTCTTCCATAACCCTCATCTTTACACGTGAGGCC TTCAACTTGGGTTCCTGCCTTGTTCTTCCTTCCTGCCTCTTGGTTCCATGTCTATTCCCTCTACTCAGGC AAGGGTGACTGACTCTTCAGCAAGTCAGGCTGTTTAGAGAACTCTTCTCATGTTTTTGTGTGATGTGTGG TACAGGTTGAACTCAGTGGGGCTGGGGAAGGCCTGAACGTTAGCATTTCTAACATGTCCCAGGTGAGGGA GCCATGGCCTCCTTTTTGGCCTGAAGAACCCTTTGAGGAGTCAGGTTCTGGATTCGAGTGTGAAGGGTTC AGATTAGAGCAGACTCTAGATCTAAAGCAAGTTTCCAGCTCTGCAAGAACTAGGGGGATGCTACCCTGCC AAGTGGAAGAGGTTTCCTGGCTTTAGACGTGCCCACAGGTGGTGGCTGAGAGGAAGTCCAGAAAGGGAAA AAAATAGGAGACACGCCTGGGCCCGAGTGTAGAATCCAAATTCACGTTTTCATTTTGCCTTCCAGTCTCA AGTATATTTTGACTTTGAAGTGTGACACAGGCCCTGGAGCTCATGACTCCTCAAGATGGGACTGGAACAG AGCATGTTCAGGCAGAGAGAGCTCTTGAGTCCACATGTTGCAAAGAAATGGCTGTGATCAGGAATCTGAA TCCTTGGCTGGGCCGTAGGTTGATAGAGTCCATTCTCAGCACGCATGAACTCATAGGTTCAGTCTTAGAC AGACAGACAGACAGACAGACAGACAGAGGAGATGAGAAGAAAAGAAAGGAAGAGAGAAGAAAAGGAGGGA GTAGGGAAGAGGGAGGGAGAGAGAAAAGAGAAGATTCAAAGTTTGCTTCCCACTTGATGTTGCCAACTGT GCAATGCGATCCGTCAGACAACACAGGGCAAGGCAAGTTTGACCACAGGTTGAGGACGTAAAAATCTGGC AGGGTTCTGGTTCAGTCTTTACAAATGAACCTAAGCAGGAGAAGGAGCTATTAGAGAGGACTAAGAGGAC GTGGGCTTCTCTTCATGGGCAACAGCCTTTAGGGTACTGAGGAAAGCTGTCCCGGGGATTGATGAGAATG GAAGCCAACACAGGACACCTTCATTCCAGAAGCCTGCAGTGCGGGCAACAGCAGGAAGCTCTTCCCGAGA AAGGGGAGCTCTTCTCCAGGAAAAGACACAGGTCACACCTCCCAACTCTCTGTCCAGGTTGTGCCATCCT GCTCTGTTAGAGTCAACTATGGAGCAGAAGAAACCCAGGTCAACCATTCCTCTTGAGGCCTCAAAGGGCC AGCCATGATTCTTCCTAGTTGCTTTGTTGAAAGGTCTGACCCAGAGTCTTCCTTCCCTTAGGAAACCCTC TTCCCTCCAACAAGTACACAAGACTACTGTTTACAAGTCGGAGTCGTCATTAAACACAAACAACTCTGGG AAACCAGATGCAGGGCTGGAAGGAGCCAAGCAAGGAGGGTACCCAGGAGGAGCTGGCAGGGCGAGAACAC CTGGAGATGAGGGGAGCAGAGTGTGTGGGTGGGGGGGGGGATGGAGAGCATCCTGTCAGACAGGTGCGAG TTGAGATCTCAGGCACCAGGTAGAGCGAAGCACACCTTGGAGTCTGAGAGACTAAACCCTGCACCTCTCA TCTACCTTGCTCAACGTTAACCTCTAAAAGCCTCAGTCCCCTTACCTGCAGAAGGAGGCTTGCTGTCCTG ACCTTGAAGGGTTGCTGAGAATGCTAGAGTGTGCAAACCATGCCTACACTAAGTAGACACTCTGGGAACA GTAGCCTGATGCTCTGAAAACATGAATAAATGAAGTGGGCGTTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30611649 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30611649 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17643 same embryo
	EMAGE:17640 same embryo
	EMAGE:17642 same embryo
	EMAGE:17641 same embryo
	EurExpress:euxassay_005980 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source