EMAGE mouse gene expression spatial database

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EMAGE:17641

Med19 mediator of RNA polymerase II transcription, subunit 19 homolog (yeast) ( MGI:1914234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005973. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005973_01	euxassay_005973_02	euxassay_005973_03	euxassay_005973_04

euxassay_005973_05	euxassay_005973_06	euxassay_005973_07	euxassay_005973_08	euxassay_005973_09

euxassay_005973_10	euxassay_005973_11	euxassay_005973_12	euxassay_005973_13	euxassay_005973_14

euxassay_005973_15	euxassay_005973_16	euxassay_005973_17	euxassay_005973_18	euxassay_005973_19

euxassay_005973_20	euxassay_005973_21	euxassay_005973_22	euxassay_005973_23	euxassay_005973_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17641_wholemount_strong.wlz

17641_wholemount_moderate.wlz

17641_wholemount_weak.wlz

17641_wholemount_possible.wlz

17641_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17641_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9538
Entity Detected:	Med19, mediator of RNA polymerase II transcription, subunit 19 homolog (yeast) ( MGI:1914234)
Sequence:	sense strand is shown >T9538 AGAGTGCTATGGAGAACTTCACGGCACTGTTTGGAGCTCAGACTGACCCCCCACCGCCACCAAGCGCCCT AGGCTTTGGGCCTGGAAAGCCACCCCCGCCACCCCCTCCTCCTCCGGGAGGTGGTCCAGGCGCAGCCCCG CCCTCGACGGCAACCTCGGCCCCCGCAGGAGCGGATAAGTCGACGGCTGGAAGTGGCCCCTTCTACCTGA TGCGGGAATTGCCAGGCAGCACAGAGCTGACGGGCAGCACCAATTTAATCACACACTACAACCTGGAGCA GGCCTATAATAAGTTCTGTGGGAAGAAAGTGAAGGAGAAGCTAAGTAACTTCCTGCCTGACCTGCCAGGG ATGATTGATCTCCCTGGCTCTCATGACAACAGCAGCCTCCGATCCCTCATTGAGAAGCCTCCTATTCTTG GTGGCTCTTTTAATCCAATCACAGGGACCATGCTGTCTGGTTTCCGCCTCCACACTGGCCCGTTGCCAGA GCAGTGTCGTCTGATGCATATTCAGCCACCCAAGAAGAAGAATAAGCATAAGCACAAACAGAGCCGCACC CAGGATCCTGTTCCCCCAGAAACACCATCTGATTCAGATCACAAGAAGAAGAAAAAGAAGAAAGAGGAGG ATCCTGAACGAAAAAGGAAGAAGAAAGAAAAGAAGAAGAAGAAGAACCGACATAGTCCAGACCACCCAGG TATGGGCAGCTCTCAAGCCAGCAGCAGCAGCAGCCTCCGCTAATAGGACTACTGGACTTGGCCAAGGATG GCTTTCCTGTGATACTGAACCTGCTGATAAAAACTTGTCTTCCAGGCAGTTGGACCCTGTTGTGGGGGCA TCCAACAGCTGGAGAGGGGCCAGCCCCTGCTGTGCATGTGACTAATACACTTTAGGGAAGGACCATGCTT TTTTATGGTTATGTCCAATTGGCATTTTCATGGATTCCTTTTTACAAGAATCTGTTTTTCTTTTTTTTCC CTCTTTTGTGCAATTTGATCCTAGGACTTCAATGTTTTGTTTTAAAGCGTGTTTAACAAACTGACCGGTA TGGCTGCCTTTTAAGAGGCAAGTGCCATTCTATTTGAATCAAAGTTTAGGACAAGGCATTAAGATGCAAG AGATTTTGGATCTTGCCATCACATTATTTCCTTAGCAGGCTGGAACTGGAAAATCAAAATGCGAATAGAG AACATGGTCTGAAAACAGTAACTTGCTACTATTTTCCTACCATAGACACAAAATACTCGCTGATTATGCT CAGAGCAGAGTGCATGGATGATCTTCACCTTTCACCTGGAAAGCTGGCCCAAATAAGGTTTATACCTGTG TTTATATACCACAGGGAACTGGTGAGCCACAATCACGGATATATTTTAGCAGGAAAAGCCAATTAGTATT TGTCTCATTATGCTCCCACCTTTTTCTGGAGCCTGACCCCTAAGACTTGTGAACACTAGGCAAGTTTTAT CTTTTCCTTTCCCTCTCTTGACTTTTTTTTCCCCCCTACATACTTTTTAAATGAACCTCTGGCAAGAAGC TTAATGCTGGATTTGGTTGTTTTGAAAAAAGTTTTGGTTTGGTTTTTGTAGGTTGTTTTTTGTTGTTGTT GTTTTTGACACATCACACTATGCAACCTTGGCTGGCTATGAACACTAGGCTGGCCCCAAGCTTGAGAGAC CCATCTGCCTCTACCCACCAAGTTCTGGGATCAAAGGCATACACCACCATGCTTAGCTGTTTTTACTTTC AAAAGGAAACAAAGGAATAGCAAGAGATTCAACAGAGAGGATGTATTTGTTATGAAAAGCTAGGTTTTTC TCACCCAGCAGGGCATTTAATTGGATAGCCAGAATAAAAACAGAACCAGAGAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:30606373 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30606373 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17643 same embryo
	EMAGE:17640 same embryo
	EMAGE:17642 same embryo
	EMAGE:17639 same embryo
	EurExpress:euxassay_005973 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source