EMAGE mouse gene expression spatial database

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EMAGE:17647

Gdpd5 glycerophosphodiester phosphodiesterase domain containing 5 ( MGI:2686926)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005984. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005984_01	euxassay_005984_02	euxassay_005984_03	euxassay_005984_04

euxassay_005984_05	euxassay_005984_06	euxassay_005984_07	euxassay_005984_08	euxassay_005984_09

euxassay_005984_10	euxassay_005984_11	euxassay_005984_12	euxassay_005984_13	euxassay_005984_14

euxassay_005984_15	euxassay_005984_16	euxassay_005984_17	euxassay_005984_18	euxassay_005984_19

euxassay_005984_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17647_wholemount_strong.wlz

17647_wholemount_moderate.wlz

17647_wholemount_weak.wlz

17647_wholemount_possible.wlz

17647_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17647_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 14

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 15 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 14

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 14 15 16

trigeminal v nerve

strong

regional

strong expression: see section 09 15

spinal cord

strong

regional

strong expression: see section 05 06 07 08 09 10 11

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 12

cervical ganglion

strong

regional

strong expression: see section 06 07 14

thoracic ganglion

strong

regional

strong expression: see section 09 10 11

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 09 10 11 12 13

neural retina

strong

regional

strong expression: see section 03 04 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9552
Entity Detected:	Gdpd5, glycerophosphodiester phosphodiesterase domain containing 5 ( MGI:2686926)
Sequence:	sense strand is shown >T9552 CGCGAGGCTGAGCAAGCGGTGAGCGGGGAGCCGGTAAGAGGAGGAAGTGGAGCGGCGGCCCGGAGCTCTG GAGTCCGCTGCCGGCACAGGTCCGCCCAGATTTGCAGAGTGAGCGGCGTCGGGACCTGGGCAGACTTCTT CGCCTTGCTCCGGCCGCTGGCGCTCCGCCTGCCCTGCCCTGCCCTGCCCTGCTTGTCCGAGCCCCGCATT CCGCGCCTGGACTTTCCTAGCCCCGTGGTCTGGAGCTGCTGGGACGTTCGAGCCCCGGCCTTGATCCGGA GTTCCGAGACGGGACGTTCTCGGCCCCATCATACTGACCCATCTCTGCTGGAGTGTCCTCCGCCCCCTGG TCGGCGCAAGGTGGCCCCAGCTCTGGGACTTGAGTGTAGAGGAGCCGGGACGCCCACCGGCCTCCAGCTC CTGGAGAACCAAATCTTCAGCTGCCTTGGACCGAACTGCTCCGGACTTGAAACTGTACTGGCACCGCCCG GGTCCAGCGCACCGTGAGGGGTCCCAAGCAGGCAGAACAGTCGGACCGAACCCCTGGGCGAGTCGAAGCT GCCACCTTTCTCCGCCCTAGGTTAAGGATCTTGGGGTGCTTGCTCCCAGGGGAACACAGGGGCTTCTCCT GCCACAGTCTCCAGCGAGCGGAGGTTGCAGGGAGGTAGCATTACCACATTCTTCATCCACCGACAGGTGA CACCACTGGAGACCGTGGGCTGTGGAAGAAACACACGGAGCACAGCTCTTCGAGTGAAGTGACATCACCG TCTCCGTGGACAGGACACCCCTGGAGTGCAGCCCATAGCCGCCTGGACCTTCTTCCAGCACCAGCCAGGG GCCTCCATGGGAGCTTAAGGGCCAGGCAGCAGGGCTGCGGGCACGAGCATGGTGAGACACCAGCCCCTGC AATACTACGAGCCCCAACTGTGCCTCTCATGCCTCACAGGCATCTATGGCTGCCGCTGGAAGCGCTACCA ACGCTCGCACGATGACACCACGCCGTGGGAGCGCCTGTGGTTCCTGCTCCTGGTCTGCACCTTCAGCCTC ACACTCACCTGGCTCTACTTTTGGTGGGGTGTCCACAACGACTACGATGAGTTCAACTGGTACCTCTACA ACCGCATGGGCTACTGGAGCGACTGGTCCGTGCCCATCCTGGTGACCTCGGCTGCTGCTTTCACATACAT CGCAGGCCTCCTGGTCCTGGCACTATGTCACATCGCTGTGGGGCAGCAGTTGAACCTGCACTGGATACAC AAGATGGGGCTGGTGGTCATCCTGGCCTCCACGGTGGTGGCCATGTCTGCAGTGGCCCAGCTCTGGGAGG ATGAGTGGGAGGTGCTGCTGATTTCCCTGCAGGGCACGGCACCATTCCTGCATATAGGGGCCCTGGTGGC CATCACTGCACTCTCCTGGATCGTCGCTGGACAGTTTGCCCGTGCAGAGCGGTCCTCCTCTCAGCTCACC ATTCTTTGTACCTTCTTCGCCGTGGTCTTCACCTTCTACCTGATCCCTCTCACCATCTCCTCTCCCTGCA TCATGGAGAAAAAAGACCTGGGCCCCAAACCTGCCCTCATCGGCCACCGAGGGGCTCCCATGCTGGCTCC AGAACACACAGTGATGTCCTTCCGGAAGGCGCTGGAGCAGAGGCTGTATGGGCTGCAAGCTGACATCACC ATCAGCCTGGATGGTGTGCCCTTTCTCATGCATGACACTACCCTGAGGCGGACCACCAATGTGGAGCACC TGTTCCCGGAGCTTGCCCGCAGGCCTGCTGCCATGCTCAACTGGACTGTCCTGCAGAGGCTCAATGCCGG CCAGTGGTTCCTCAAGACCGACCCCTTCTGGACGGCCAGCTCCCTGTCACCCTCCGACCACAGGGAGGTT CAGAACCAGTCCATCTGCAGCCTGGCAGAGCTGCTGGAGCTGGCCAAGGGCAATGCCTCGCTGCTGCTCA ACCTTCGGGACCCGCCCCGTGATCACCCCTACCGTGGCAGCTTCCTCAACGTCACACTGGAGGCCGTGCT GCGTTCAGGCTTCCCCCAGCACCAGGTCATGTGGCTATTTAACAGGCAGAGGCCTTTGGTACGGAAGATG GCTCCTGGCTTCCAGCAAACATCTGGATCCAAAGAAGCCATCGCTAACTTACGGAAAGGTCACATCCAGA AGCTGAACCTCCGCTACACTCAGGTGTCCCACCAGGAGCTCAGGGACTATGCATCGTGGAACCTGAGTGT GAACCTGTACACTGTCAATGCACCGTGGCTCTTCTCCCTGCTGTGGTGTGCTGGGGTCCCCTCCGTCACT TCCGACAACTCCCATACCCTTTCTCGGGTGCCTTCTCCACTCTGGATCATGCCCCCAGACGAGTACTGCC TCATGTGGGTCACTGCCGACCTGATCTCCTTCAGCCTCATCATCGGCATCTTCGTGCTCCAGAAGTGGCG CCTGGGTGGTATACGGAGTTACAACCCAGAGCAGATCATGCTGAGCGCCGCTGTACGCCGGACCAGCCGG GACGTCAGCATCATGAAAGAAAAACTCATCTTCTCAGAGATCAGTGATGGTGTGGAAGTCTCCGATGAGC TCTCCGTATGTTCAGACAGCAGTTACGACACCTATGCTAATGCCAACAGCACAGCCACCCCCGTGGGCCC TCGAAATGCTGGCAGCCGTGCCAAGACCGTCACAGAGCAGAGTGGGCATTAGCTGAAGATCTGTCTGCCC AACTTGTACCCAAGGTAGAACCTAGAAGAGAGGAAGGTCTGATGAAAAGAAGCTGCACCTAGAGGGCCCG GGAGCTGGCTCCTCAGCCTACTTATGTGCTCCAAGCTCCTTTGTCTGTTCCAGTCTCTCTTGCATGAGTG GGGGGGTGACCAAAGGGGCCCTCTCCGCTGAGGCTCAGCTGGACCAGGACTCCAGCCTCTAGATGCCCCT GCAGGCCTGGGGCTTTTCATCCAGGAAGCGAAGGCCTGATCTCCTGGTCCACTCTCATAGTCATCCTTGG CCTCCCAGCCTGGAAGGGTTGGTGAGTCACTGGGCTGTGCCGTGTGCCACAAGCAAGTGGAGGACGTGGA TGTCTCCCTATGCCCACACCTCTCTATTTATCTGTCTGTGCTGTGAGATGACTAATGTTCTCACCCCTGC CTTGAGGACGTTGGCTGGGCCTCCCTCTTGGGGGGGGGGGGGTGTTTCTACGCTCTGCACCCATCTCAAA GCACAAGGAAGTTCATCTCAGGAGAAGAAAGCAGTGGCTGTGGTGGAGATGCGTTCCTCCCAACCAGCTC CCTTCTACCACTGACCTCTCTGTCCCTGAGAAGCAGCTGGAGAGGACCGGAATCACAGGCTCAGGGCTGT CCTGCCTCACACCCAACTGTCCAGCAGGCCTCTGGGGCAGAAGAAGCCTTGAGGACCAGGTGAACGACAG ACTGATCTGACCTCAGGTTCCCACAGCAGCGGCCACAGGGCAATGGGCCACCTGGGCGAAGCACATTGGG CTCAGTCAAGCATGGGCCTCTGGCCAAAGTTGCCTACAGTAGAAGGAACCCAAGGGCAAAGACAATTAAA GCTGCCATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30608279 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30608279 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17646 same embryo
	EMAGE:17648 same embryo
	EurExpress:euxassay_005984 same experiment
	MGI:4825040 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source