EMAGE mouse gene expression spatial database

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EMAGE:17648

Fam70a family with sequence similarity 70, member A ( MGI:3045722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005979. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005979_01	euxassay_005979_02	euxassay_005979_03	euxassay_005979_04

euxassay_005979_05	euxassay_005979_06	euxassay_005979_07	euxassay_005979_08	euxassay_005979_09

euxassay_005979_10	euxassay_005979_11	euxassay_005979_12	euxassay_005979_13	euxassay_005979_14

euxassay_005979_15	euxassay_005979_16	euxassay_005979_17	euxassay_005979_18	euxassay_005979_19

euxassay_005979_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17648_wholemount_strong.wlz

17648_wholemount_moderate.wlz

17648_wholemount_weak.wlz

17648_wholemount_possible.wlz

17648_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17648_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9557
Entity Detected:	Fam70a, family with sequence similarity 70, member A ( MGI:3045722)
Sequence:	sense strand is shown >T9557 GAGAGGCAGCGGCAGAGTAGGATGCTCTGCGGGGCGCCCTGAGCCAGGGGCTCTTGAAGCAGCTTCGGGG ACAGGGGCAACTGGACAGCTTTCACCATGCATCAGTCCCTGACTCAGCAGCGGTCCAGCGACATGTCCCT TCCCGATTCCATGGGAGCCTTCAATAGGAGGAAGCGGAACTCCATCTACGTCACCGTTACTTTGCTTATT GTGTCCATGTTAATTCTCACGGTGGGCCTTGCTGCGACTACCAGGACCCAGAATGTGACCGTGGGAGGTT ATTACCCAGGAGTTATTCTTGGCTTTGGATCATTCCTTGGGATCATCGGGTCAAACCTGATTGAGAACAA GCGGCAGATGCTGGTGGCTTCTATCGTGTTTATCAGCTTTGGTGTGATTGCAGCATTTTGTTGTGCTATA GTTGATGGGGTCTTTGCCGCCAGACACATTGATTTGAAACCACTCTATGCTAACCGGTGCCACTATGTTC CCAAGACATCACAGAGGGAAGCTGAGGAGGTCATAACTTCCTCATCCAAAATTACTCCTTCTACGAGGGC TTTGAGGAACCTTACCCAGGCAGTTAAAGAGGTGAACTGCCCTCAACTTAGCCGTGGACTTTGCACACCT CGCATCCGGGGCAACACCTGCTTCTGCTGTGACCTCTACAACTGTGGCAACAGGGTGGAAATCACCGGCG GCTACTATGAGTACATTGATGTCAGCAGCTGCCAGGACATCATCCACCTGTACCACCTACTCTGGTCTGC CACCATCCTCAACATCGTTGGCCTGTTCCTGGGTATCATTACTGCTGCGGTCCTTGGGGGATTTAAGGAT ATGAACCCTACTCTTCCGGCACTGAACTGCTCTGTTGAAAATGCCCATCCAACTGTTTCTTACTATGCGC GTCCCCAAGTGGCATCGTACAATACCTACTACCATAGCCCTCCTCATCTACCACCGTATTCTGCTTATGA CTTTCAGCATTCCGGTGTCTTTCCATCCTCTCCTCCTTCTGGACTCTCCGATGAGCAGGAGCCCCAATCG CCCTCTCGTTCACCCAGCTACATGTGGTCCTCCAGTGCGCCACCCCGTTATTCTCCACCCTACTACCCAC CTTTTGAAAAGCCACCACCTTACAGTCCTTAAAGAGGAATACCTGTCGGCTATTGAGATTATTGTGGCTT TTGTATTTCTGCTCTAGTGGAAGTGTGTAGGTTACATCTAAAGTGTGACTCTTCTACATCAAGTTTTACA ACAGCTTGCCAAGCTAGGGAAAGATAGGGATGAAGCTTACTCATTATCAAGGAAGGACAGGATTGGCTAG GCCAAACCTAGTACTTCGAAGAAAGCAAGTTCACTAAGCAGGGCTGGGGAGCCAGTCCAGCAAGAAGCAT GGCTTCTTCAGATACGCATTTCCTGATGCTCCGCCTCTATACAGTACAAGTGTGGTTGCCATCTGTTTCA CTTTAAGTAAACGACAGCAGTCCTCGGGCCCTTGGTAGATGACTATGATAGCACCTTGGTTGAATCACCT GGTCTATAGGCTCTATCTTTCCCTACAATTGATGTCACTCTTAGGAAAATTTCGTAGCTGATTGGGCTAC ACAGTAGTTCCAATACAGAAAGTTGTGGCTTTTTGTTTGCTCGTGTCTGGATATTGAAAAAAAAAAGCTG CCAATCTCTTTACTCTGTTCTCCAAGTCGTGTAAAAATGGCTGTTGTGATCATTCAGCTTTTGTGGCTGG TACCTCCTAAGGGGAAAAGTGCAAGAGTCCCTTCTCCTGAGTGGTGAGAGACCATGACCGGCTGATGGAG AAGCACGGAAATACAGAGCAACATGTGTGCTGTTTTCGGTAGATCTCAGCATACATCTGGCAACTTAGGA GACAGTGTTCCTACTATATGTTGCATTATTACAAAACACATTTGCTACTGGAGATAGACATCTTACAGTT GAAATTCAGAGTTTAAAAATGCTAGATTAGATTGGATCGTGGTATGAATTGTTACTTATCTTTGTGACTA TTTAATCTTCAGCTATTGTGCTTTCACCCCAGCAAACCGCACGGTCCTGCACCCCACCACTGAAAACTCA TCTGTATTTTAATGCCACTGCTCTTATTGGTCCCTTTTTTCTGTTGAGATCAATTGTGACAGCATTCAAT TCAAGATTATGAAAATGCTACAATGCCATTTCAAGTCTTCAAGACCTCAAATATAGCCAACTTGACAAAC TCTTAAGTGTTACGCATTTAAAAGTCCACCGGGCATAGTGTGGTAGTTATATAGTTGTTTTAATGATACA GAGCTTTAAACCATCAAACTGTTGAGACTCTAGCACCCATGCCTTCACTCCAGCATGAACACATTCACTG GGATTTTATTTTAACCTGAGAAATGGTTTAAAAGAAGATTGATAATCCTACACTTGTATTATGTAAAAGT ACAGGAGCTACAGAGGACGCCTAATTAGACAGTTCTGCAAACACAAGAACACACACTGGAAACATTTCCA GCCCATTTCGCTGCCTCCCTTCTTTGATGGTTGGAGGCAGCAGACCAATGCTGGTGCTCCCATCTACCTT GTAGACACCTGGTCATCAAGAATCACCAAGGCACAACAAGTGCACTCACTGTTACGGTAAATGTTTGCAA AACATTCAGTTTAACTTTCAATGTCTCTTATCCAGTCTTCAAATCCAAAAGACTACAATCCTTTTATGTT ACACAAAATTACTGTTATTTTTTCAGTTCTGAACATATTAAAATTCTATTGGGCTTCAAAAAGGACTAAT AATCAATTGACTTATAGAGATACCTAAGGGTTTTTTCTTCTCACTTACACTTTACCCCTTGCTGTAGGGA ATGTGTGTATTTTAAAGGCACCATTACAAAGTATATCTTTTATGATCACATTTCTTAAAGTGTTAAGATT CTATGACTCATTTCTATGTATTTTTTTTACTTTACAAAATAACTTGAAACAGTATAGATTTTGTAACACT TAATTTGAGCAGCTTTTTTTTTAATCACATTGAATTATATCAAGTGCATGCTACCTTAGGAAAAATTATA TTTGCTGCTTCACTCTTCTGCAAAACATTTGCTGTAATGAAGGAATTTGTATTTCCAATGTATCTTGACG GCATTTTATAACATTTACTGCTTTCTTCCTAATTCTGCTCTAAAGTATTGAACTTGGCATGAAATATTAA AATATTAATTCAAAACCTGTATAAAATAGATGTTGCTTAAAATTTGTATCACTGCCATGTTGGAAACCCA GACAGCTTTTGTGATGTTTCAAATTAATAAAATTATCTTCCCTATCCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30550174 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30550174 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17646 same embryo
	EMAGE:17647 same embryo
	EurExpress:euxassay_005979 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source