EMAGE mouse gene expression spatial database

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EMAGE:17650

Dqx1 DEAQ RNA-dependent ATPase ( MGI:2136388)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005983. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005983_01	euxassay_005983_02	euxassay_005983_03	euxassay_005983_04

euxassay_005983_05	euxassay_005983_06	euxassay_005983_07	euxassay_005983_08	euxassay_005983_09

euxassay_005983_10	euxassay_005983_11	euxassay_005983_12	euxassay_005983_13	euxassay_005983_14

euxassay_005983_15	euxassay_005983_16	euxassay_005983_17	euxassay_005983_18	euxassay_005983_19

euxassay_005983_20	euxassay_005983_21	euxassay_005983_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17650_wholemount_strong.wlz

17650_wholemount_moderate.wlz

17650_wholemount_weak.wlz

17650_wholemount_possible.wlz

17650_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17650_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9559
Entity Detected:	Dqx1, DEAQ RNA-dependent ATPase ( MGI:2136388)
Sequence:	sense strand is shown >T9559 CACTCCAAAACTACAAGTCCCAGAATGCATTTCATGACTATAGTCATATAGGCTCAGTTATACAAGCTCA GGGTGGCATCAGGAACCTGCTTTCCATAGGTTCCTAGCTAAGCTGACCAAGGTGGAGCAAAAGGCCCCTC CCCCCAAAATAAAGCTCTGGGATTGGAGTTTGAGCTCAGCCTCCCGGATGGGGCCGGAATTTACCTTTGT ACTCCAGGGAGGCCTGGGTTGGTGTGCCTGGCTGGGTGTGTCCAGAACTGGCTGCCATCCTGACCGTGTC CCAGAGCTGCTGGCAGGTTTGAATTCAGTATCAGTCACCAGACCCAAGGCCTTACTGGCTGCCCTGCTTT TCGTAGGTTCTATGAGCAGCCACCATGGCCTCTGGGCTGGCAGAAGAGTCTGAACTGAGTCCTGGGGAGT CTGAGCTGGCTGTGAACCCCTTTGATGGGCTCCCCTTCTCCTCCTGCTACTATGAGCTGCTTGAGCAGCG TAGAGCCCTGCCCATCTGGGCTGCTCGCTTCCTGTTCTTGGAGCATTTGGAGAGTAGCCCCACTGGAGTG GTGCTGGTATCTGGGGACCCTGGCTCTGGCAAGAGCACCCAGATTCCTCAGTGGTGTGCAGAGTTTGCGC TGGCCAGAGGATTCCAGACAGGACAGGTTACAGTCACTCAGCCCTACCCTCTGGCAGCCATGAGCCTGGC TTCAAGGGTGGCTGATGAGATGGACCTGACCCTGGGTCATGAGATTGGATACAGCATCCCTCAGGAAGAC TGTACTGGGCCCAACACCATGCTCAGGTTTTGCTGGGACAGGCTATTTCTGCAGGAAGTAGCCTCAACCC GGGGCCCTGGAGCCTGGAGTGTGCTGATACTGGATGAGGCTCAGGAGCGGTCAGTGGCCTCAGATTTACT ACAGGGGCTCTTGAGAGATACCAGACTGAGAAACCTGCCAGGGGATCCCAGAGTGGTTGTAGTTACCGAC CCAGCGCTTGAACCAAAATTCCAGGCTTTCTGGGGCAATTCTCCCATTGTGCGTGTGCCCAGGGAGCCTG GTGGAGATCCCACCCTTGCCTACAAGGACACTGTCCCTACCGACCTGGTGGAAGCTGCCTGCCAAGCTGT GCTTGAGCTGTGTCAGCAGGAAGAGGCACCAGGAGATGTGCTGGTGTATCTGCCCAGCGAAGAGGAAATT TCTCTGTGCTGTGAATCCTTGTCTGGGGAGATGGGAACGCTGGCTGTCCCAGGGCCTCCACCGCGGGTGC TACCCCTTCACCCTGGCTGTGCTCAAGCCATCCAGACAGTGTATGAAGACACAGACGTGAGTGTTCGCAA AATCGTAGTCACTCATTGGCTGGCCGATTTCTCCTTCTCCCTTCCTTCCATCCAACACGTCATTGACTCA GGACTGGAACTTCGTAGTGTTTACAATCCTCGGATCCGGGCAGAATCCCAGGTGTTGAGACCAATTAGCA AGTGCCAGGCAGAGGCAAGACTGCTTCGGGCAAAGGGGTTCCCACCAGGATCCTGCCTCCGCCTGTATTC TAAATCCACCCTAGAACTAGAGGCTCCCCCACTGCCCCACCCCAAAGTGTGTGAAGAGAATCTGAGCTCT CTGGTGTTGCTACTAATGAGGAAACAGATTGCAGAGCCAGGGGAATGCCACTTCCTGGACCGGCCTGCAC CAGAAGCACTGATGCGGGCCCTGGAAGACTTAGACTATCTGGCTGCTCTGAATGATGACGGGGAGCTGTC GGACCTCGGAGTGATCCTGTCGGAGTTTCCCCTGCCCCCGGAGCTGGCCAAAGCCCTGCTGGCCTCCTGC GAGTTTAACTGTGTGGATGAAATGCTCACTCTGGCTGCCATGCTCACTGCTGCTCCTGGGTTTACCCGGC CTCCGCTGAGTGCGGGAGAAGCTGCCTTGCGCCGGGCTCTGGAACACGCAGACGGTGATCACAGCTCTCT GATCCAGGTCTATGAAGCCTTTGTACAAAGTGGAGCAGATGAAGCTTGGTGTCAGGCTCGGGGTCTAAAC TGGGAGTCATTATGTCAAGCCCGTAAACTCCGAGCAGAGCTTGTGGAACTCATGCAACGGATTGAGCTTC CTTTGTCCCAGCCAGCCTTTGGCTCTGAGCAGAATCGCAGAGACCTTCAGAAAGCACTGCTGTCAGGATA CTTCCTCAAGGTGGCCCGAGACACAGATGGGACAGGAAATTATCTGCTTCTCACCCATAAACACGTAGCC CAGCTGTCTCCATACTGCAGCTACCGAAACCGCAGGACTCCAGCCCAGCCACCCACGTGGGTGCTGTACC ACAGCTTCTCCATCTCGAAAGACAACTGCCTTTGCATTGTCTCTGAGATCCAGCCTGAGATGCTGGTGGA GCTCGCCCCTCCATACTTCCTGAGTAACTTGCCCCCCAGTGAGAGCAGAGACCTCTTGAACCAGCTGAGA GAAGGAACAGCAGAGCCACCAGCAGCAGCGACTGAAACTTCCTCTCCCCAGGAGTATGGAGATGGCTGTG TTCTGCAGTGAGCTAACTGCCTAAGGAATGGAGCCAAACCTCATCAGAGAGCGCCAAGGCAGTCAGACTT AGAGACCCAAAGTGTAGGGTCAGATATACTCCTGGAACCCAAGAGCCAAGAACTGTTAGGAGTGGGGGAA GAGAGCGCCACAGTTCAGAACAAGCATACTTTTCTTCTCTGTCCATGGACATGGGAGCAGATGCCACTCA CCCCACCCATGCGGCGCCTCACACAACCTTGTCTCCTTGGTGAGACTATAAAAGACTTTGGTGGGGGGTG GAGAGATGGCTCAGGGGTTAAGAGCACTCACTGACTGCTCTTCCAGAGGTCATGAGTTCAATTCCCAGCA ACCACATGGTGGCTCATGACCATCTGTAATTGGATCCAGTACCCTCTTCTGGTGTGTCAGCTACAGTGTG CTCATATACATTAAATAAATAAATCTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTCGAGACAGGGTTTCT CTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGTTAGCCTCGAACTCAGAAATTCACCTG CCTCTGCCTCCCAAGTGCTGGGATTAAAGGCATGTGCCACCATGCCCATCTAAATAAATCTTTTTTTTAA AAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30612145 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30612145 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17649 same embryo
	EurExpress:euxassay_005983 same experiment
	MGI:4824392 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source