EMAGE mouse gene expression spatial database

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EMAGE:17651

Pdcd11 programmed cell death 11 ( MGI:1341788)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005987. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005987_01	euxassay_005987_02	euxassay_005987_03	euxassay_005987_04

euxassay_005987_05	euxassay_005987_06	euxassay_005987_07	euxassay_005987_08	euxassay_005987_09

euxassay_005987_10	euxassay_005987_11	euxassay_005987_12	euxassay_005987_13	euxassay_005987_14

euxassay_005987_15	euxassay_005987_16	euxassay_005987_17	euxassay_005987_18	euxassay_005987_19

euxassay_005987_20	euxassay_005987_21	euxassay_005987_22	euxassay_005987_23	euxassay_005987_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17651_wholemount_strong.wlz

17651_wholemount_moderate.wlz

17651_wholemount_weak.wlz

17651_wholemount_possible.wlz

17651_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17651_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9583
Entity Detected:	Pdcd11, programmed cell death 11 ( MGI:1341788)
Sequence:	sense strand is shown >T9583 CCCTGGTCTTACCTACGCTCGTGGACGAGTCGCGTGGAGCTGTCGGCTCCCTGTTCTGCGCGTGCGATTG CGTTGAGACGCTCCGTGAGCTGCAGAAGACCCCAACATGGCAAACCTGGAGGAAAGCTTCCCCCGAGGGG GGACACGGAAACTCCACAAATCAGAGAAGTCTTCCCAGCAGGTGGTTGAACAAGACAACTTATTTGATGT TTCCACAGAAGAAGGTCCCATTAAAAGGAAAAAGAGCCAGAAAGGGCCAGCAAAAACAAAAAAATTGAAA ATTGAAAAGAGAAAATCCATCAAATCTATAAAAGAGAAGTTTGAAATTCTTAGTCTTGAGTCCCTATGTG AGGGGATGCGGATTTTGGGCTGTGTGAAGGAGGTGAGTGAGCTGGAGCTGGTGGTCAGCCTTCCCAACGG CCTCCAAGGGTTTGTGCAAGTGACCGAAGTCTGTGACGCCTACACTCAGAAGCTGAATGAGCAGGTGGCA CAAGAAGAACCTCTGGAGGACCTGCTCCGCCTGCCTGAGCTCTTCTCCCCTGGAATGTTGGTGAGGTGTG TGGTGAGCAGCCTGGATGTCACAGAGAGTGGCAAGAAGAGCGTCAAGCTTTCTGTGAACCCCAAACGTGT CAACAAAGTGCTGAGTGCTGATGCCCTGAGGCCTGGCATGCTGCTGACAGGCACAGTGTCAAGCCTGGAA GACCATGGCTACCTAGTGGACATTGGCGTTGGTGGGACCAGAGCTTTTCTGTCACTTAAGAAGGCACAAG AGTACATCCGGCAGAAGAACAAAGGTGCTAAGTTCAAAGTCGGCCAGTACCTGACCTGTGTGGTTGAAGA GGTGAAAAGCAACGGAGGAGTTGTGAGTCTCTCTGTAGAGCACTCCGAGGTTTCCTCTGCCTTCGCAACT GAGGAGCAAAGCTGGAACCTGAATAACTTACTACCAGGGCTGCTGGTCAAAGCCCAGGTGCAGAAGGTAA CTCAGTTTGGACTCCAGCTAAATTTCCTTACGTTCTTCAAGGGCCTGGTTGACTTCATGCATCTGGAGCC CAAGAAAATGGGATCTTATTCTTCAAATCAAACGGTAAAAGCCTGCATCCTCTGCGTCCATCCTCGCACC AGAGTTGTGCGACTGAGCCTGCGCCCCATCTTCCTGCATCCTGGGCGTCCACTCACCCGCATCTCTTACC AGCAGCTAGGGGCTGTGCTAGATGATGTCCCTGTTCAGGGTTTTTTTAAGAATGCGGGGGCCATCTTCAG GCTGAAGGATGGGGTACTGGCCTACGCCCGGGTCAGCCATCTCTCTGATTCTAAGAAAGCATTCAACGCT GAGGCCTTCAAACCGGGGAGCACTCACAAGTGTAGAATCATTGACTACAGCCAGATGGATGAGCTGGCCT TGCTCTCTCTGCGGAAATCAATTATTGCAGCTCCATTCCTTAGATATCATGACATCAAAATAGGGACAGT AGTAAAGGGCACAGTGCTGGCCATAAAGCCATTTGGGATCCTGGTGAAGGTGGGTGAGCAGATAAAAGGC CTGGTGCCCTCCATGCACCTGGCTGACATCATGATGAAGAACCCAGAGAAGAAGTACAGCCCAGGGGATG AGGTCAAGTGCAGGGTTTTGCTCTGTGACCCTGAAGCCAAGAAGCTAATTATGACCCTGAAAAAAACCCT TGTCACATCCAAACTGTCTCTCATCACCTGCTATGAGGGTGCCAAGCCTGGCCTGCAGACGCACGGTGTT ATCATACGGGTCAAGGACTATGGCTGCATTGTGAAGTTCTACAACGATGTGCAGGGGCTAGTGCCTAAGC ATGAGCTTAGCACCCAGCACATCCCGGACCCAGAGACGGTCTTTTACACTGGCCAGGTGGTAAAGGTGGC AGTGCTGAGCTGTGAGCCTTCCAAGGAGAGGATGCTCCTGTCCTTCAGACTGTTGAGTGACTCGCGGCCA AAGGATCCGGGTGTTGAGAGCAGTCAGAAGAAAACAGGAGCTGTGCGCATCGGGCAGTTGGTGGATGTGA AGGTCCTGGAGAAGACCAAAACCGGGCTGGAGGTGGCCATCCTGCCCCATAACACCCCTGCTTTCCTCCC CACGCCACACCTGTCCGACCACGCTGCCAATGGCCCACTGTTGCACCACTGGCTCCAGACAGGTGACACC CTTCACCGAGTTCTGTGTTTGAGCCAGAGTGAGAGACACATTCTTCTTTGCAGGAAACCAGCTTTGGTCT CCACAGTAGAAGGTGGCCAGGACCCCAAGAGCCTCTCAGAAATCCAGCCCGGAATGTTGCTCATTGGTTT TGTGAAGTGCATCAAGGAGTATGGCGTGTTCGTCCAGTTCCCCTCAGGTCTCAGTGGACTGTCCCCCAAA ACTATCATGAGTGACAAGTTCGTAACCACTCCAAGTGAACACTTTGTGGAGGGTCAGACAGTCGTTGCTA AGGTGACCAATGTGGATGAATCGAAGCAGAGGATGCTGCTGTCTCTGCGGCTGTCAGACTGCAGTCTGGG GGATTCGGCCAGCACCAGCTTCCTCCTCCTGTGCCAGTGCCTGGAAGAGCTGCAGGGCATTCGCAGCCTC ATGAGCAACCAAGACTCTGTATTGATCCAGACACTTGCTGACATGACCCCGGGGATGGTCCTTGATGCCG TGGTCCATGAGGTGTTAGAAGATGGTTCAGTGGTGTTCAGTAGTGATCCTGTGCCTGACCTGGTCCTGAG AGCCAGCAGATACCACCGGGCAGGGCAGGAGGTGGAGCCTGGACAGAAGAAGAAGGTCGTGGTTCTACAC GTGGACATGTTGAAGTTGGAGGTCCACGTCTCCCTTCACCAGGACTTGGTGAACAGGAAAACGAGAAAGC TGAGAAAAAGCAGTAGACACCAGGGAATCGTACAGCATCTGGAAGAGTCCTTTGCCGTGGCATCCTTGGT GGAGACTGGCCACCTTGTGGCTTTCTCTCTGATCTCTCACCTCAATGATACCTTCCACTTTGACTCTGAG AAGCTGCGTGTGGGGCAGGGTGTCTGCCTTACCCTCAAGACCACAGAACCGGGAGTGACCGGTCTGATTT TGGCTGTGGAAGGGCCAGCTTCTAAGAGGACCAGGATGCCAGTTCAGAGGGATTCAGAGACAGTTGATGA CAAAGGAGAGGAGAAAGAGGAGGAGGAGGAGGAGGAGGAGAAGGAAGAAGAGAACTTGACTGTGAAGTCT AAGAAAAGGCATAGCCTGGCTATTGGGGACAAAGTCACTGGGACCATTAAGGCTGTGAAGGCCACACATG TTGTTGTAACCCTAGCTGATGGCTTTGTTGGCTGTATCCATGCCTCCCGCATCTTAGATGATGTCCCAGT GGGCACCTCGCCTACGACCACACTGAAAGCCGGGAAGAAAGTCACTGCGAGAGTGATCGGTGGGCGAGAT GTGAAGACATCTAAGTTTCTCCCAATAAGTCACCCCAGATTTGTTCTGACCATCCTGGAGCTGAGTGTTC GGCCAAGTGAGTTGAAGGGTAGCTACAGTGCTCTTAATACTCACTCTGAGAGCCCCGTGGAGAAGATCAG GCAGTACCAGGCTGGGCAGACTGTGACCTGCTTCTTCAAGAAGTACAACGTGATGAAGAAGTGGCTTGAA GTGGACATCGGACCGGACATCCGAGGGAGAATTCCCTTGTTGCTCACTTCCCTCAGCTTCAAGGTTCTGA AACACCCAGACAAGAAGTTCCAGGTTGGGCAGGCCATCGAGGCCACCGTTGTTGACCCAGACGTCCCCAG GGCTTTCTTATGTCTGTCACTCATAGGACCTTACAGACTTGAAGAAGGTGAAGTGGCCATGGGCCGAGTG ATGAAGGTTGTACCCAACAGGGGACTGACCGTCTCCTTCCCTTTTGGGAAGATAGGGAAAGTCAGTATGT TTCACCTGAGCGACTCGTACTCCGAGGCACCCCTGGAGGACTTCTGCCCCCAGAAGATTGTCAGATGTTA CATCCTGTCCACAGCACACCGTGTGCTGGCTTTGTCACTCCGGTCATCCAGGACAAACAGAGAGACAAAA AACAGAATAGAAGATCCAGAAATCAACTCCATTGAGGACGTGAAGGAAGGGCAGCTCCTGCGGGGCTATG TGAAATGTGTCCTGCCAAGCAGTGTGATCATAGGCCTTGGACCTTCAGTTCTGGGTTTGGCCAAGTATTC ACATGTCTCTGAGTGTGTCCCACCAGAGAAAGAGCTTTATAACGGATGCCTCCCTGAGGGAAAGCTGGTC ACTGCCAAGGTCCTGCGCGTGAACCCCATGAAGAACCTGATAGAGCTGTCGCTCCTTCCCAGCGACACTG GGAGGCCAGACGTGTTCTCTCCTGCTCCAGAACCAAAACAAGAGGAGAGGAGCGGAGGGGCTGAGGAGGG CCAGAAAAGGAAAGAGAAGAACCAGAAGAGACGGGAGGAGAAGGAAGAGCCCCAGAAGTCACAGAGGGGT GGGCGAGGCAAGCGGGAGCGCCAGGAGTCTGAGAGCGAGCAGGAACTAGTGAACAAGAGGCCAAAGAAGT CTGGAGCCGCAGAAGAAGACGACAGTGGTGTGGAAGTGTACTACCGGGAAGGAGAGGATGAGGTGGGGGA GCCGAAGCTGCCACCCAGGGGGAAGCAGACAAAATCCACAGAGGTGCCCCGGCTGCATCTCTCTTCAGGC TTCCTTTGGGATGTGGGGCTTGACTCTCTGACTCCAGCCTTGCCACTCCGAGAAGAGAGCTCAGACAGCG AGGATGAGCAGCCGCACCAAGCTAAGAAGAAGAAGGGCAAGAAGGAGAGGGAGCTGGAGAAGCAGAAGGC AGAGAAGGAGCTGTCGCGCATTGAGGAGGCACTCATGGACCCTGGGCGACAGCCAGAGTCTGCGGATGAC TTTGACCGGCTGGTGCTAAGCTCTCCCAACAGCTCCATCCTTTGGCTGCAGTACATGGCTTTCCACCTAC AGGCCACGGAGATCGAGAAGGCCCGGGCTGTGGCTGAGAGGGCCCTGAAGACCATCTCATTCAGAGAGGA GCAGGAGAAGCTGAACGTGTGGGTGGCCCTGCTGAACCTGGAAAACATGTACGGCTCTCAGGAGTCCCTG ACTAAGGTCTTCGAGCGAGCTGTGCAGTACAACGAGCCTCTCAAGGTTTTCCTGCATCTGGCTGACATCT ACACCAAGTCCGAGAAATACAAGGAAGCTGGTGAGCTGTACAACCGGATGCTGAAACGGTTCCGGCAGGA GAAGGCTGTCTGGATCAAATATGGTGCCTTTGTTTTGGGGAGGAGTCAGGCTGGGGCCAGTCACCGAGTG CTGCAGCGAGCCCTGGAGTGCCTGCCCGCCAAGGAACATGTGGATGTGATCGTCAAGTTTGCCCAGCTGG AGTTCCAGCTCGGGGACGTGGAGCGCGCTAAAGCCATTTTTGAAAACACACTGAGCACCTACCCCAAACG CACAGACGTCTGGTCAGTCTACATCGACATGACCATCAAGCACGGCAGCCAGACAGCAGTCAGGGACATC TTTGAGCGGGTCATTCACCTGAGCCTGGCGCCTAAGAGGATGAAGTTCTTTTTCAAGCGCTACCTGGACT ATGAGAAGCAGCACGGCACGGAGAAGGACGTGCAGGCGGTCAAGGCCAAGGCCTTGGATTATGTGGAGGC CAAGAGCTCGGCGCTGGAGGACTAGGAGCACAGGCTCCGCCGGACGGACAGACAGCGGAGGCAGCAGGCA GCCTAACCCATAGCCGGGCGGGTGCTGGGGCTGTCCCAGGACTGCTTTAGTTGCTGTTGTTTCTGCAGCA TGCCTGTGGGCTGTGGTGCTCACAGAAGGCAGACTTGGCTCACTAAGGCTGAGTTCCTCATCTTGTCAGA GTAAAAAAAAACTGTCAAAAGATTGTTTTAAAATCTTTTGAGACAGGGTTTGTATTATGGTCTTGGCTGG CCTGGAACCTGCTATGTAGACTCAGCCAGCCTTGAACTTAGAAGGGATTAAAGGCACATACGATCACTTT TTACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30628812 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30628812 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17655 same embryo
	EMAGE:17654 same embryo
	EMAGE:17653 same embryo
	EMAGE:17652 same embryo
	EurExpress:euxassay_005987 same experiment
	MGI:4827111 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source