EMAGE mouse gene expression spatial database

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EMAGE:17653

1200009I06Rik RIKEN cDNA 1200009I06 gene ( MGI:1921363)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006004. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006004_01	euxassay_006004_02	euxassay_006004_03	euxassay_006004_04

euxassay_006004_05	euxassay_006004_06	euxassay_006004_07	euxassay_006004_08	euxassay_006004_09

euxassay_006004_10	euxassay_006004_11	euxassay_006004_12	euxassay_006004_13	euxassay_006004_14

euxassay_006004_15	euxassay_006004_16	euxassay_006004_17	euxassay_006004_18	euxassay_006004_19

euxassay_006004_20	euxassay_006004_21	euxassay_006004_22	euxassay_006004_23	euxassay_006004_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17653_wholemount_strong.wlz

17653_wholemount_moderate.wlz

17653_wholemount_weak.wlz

17653_wholemount_possible.wlz

17653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9567
Entity Detected:	1200009I06Rik, RIKEN cDNA 1200009I06 gene ( MGI:1921363)
Sequence:	sense strand is shown >T9567 AGAGAGTGGGTCAGGGCGGTCCTGTGGCTGGGAAGTCCCAGCTGTTGGTCCACCAGTTGGGCTGTGCTGG CAGCCATCTCTCTCCTGGACCTGGTCTCAGGATCCTGCTTGGCTGGCTTGCTTAGGACCTCTGCTGCCTG GGTGCAGTTGTCTGCGTGCCTATCAGATCCCAGGCTCCGGCCCCAGAAGGGAAAGCCGCTTAAGAAGCTT CCGGTTTGAGCAGCCAAGATCCTGCCTTTCGAGCTGAAGGGCTCTCCTGCTGCCAAGATGCCTTTGCCTC AGACAGGGGCCCCTGGGCCAGAGGTAAAAAGCCCCAGGGAGCCTAGGAAGTCACAGACCCTCCCTGTGAC CACCTGGAAGTCCAACAGTATGAAAGAGCAGAGCGTGCACCACGGGGGCAGCTTGAGACCCAGCCTGGGC ATGCTGAAGCAGACCCTCTTCCGGACCAGCCTGAGAACTTCAACCCACAAGCCCAAGGAGGACCCAGGCC TGTTCAGGCGCAGCTCCCGTTTCCTGTTCAGGTCCTTGAGACGGGCCATAGATGAAGGCTTAACTGCTGG GCATCCCCAAGGCCCTGCGGTACCAGAGAAGCCCTCCAAAGTCACAGATGGCGTCAGCAGGCAGGCAGCC ACTGGGACAGAGGCTGAGGATCTGGAACCCCAGGCAGAAAGTAAATCCGTGGCAGACCTCATCACTGAGC GGCAGCTAGTGAAGGCCTTTGAACAATTGCGGTACCTGGAGACGCAGCTGGTAGCCGACAAAACTTCCCG CACCTTTACACAGGACCCCACCGCCTATGCTCGGCGCGCTATGGACCTTTGTCTACATTACGACGGAATG GCTGCGGAGATCGGAGCCATTGTGCGCGAGGCGCTGAGTTCCGAAGGCGTGGATCGAGACGCTCTAGCGG AACTGGCCCAAGTGGTACACTTGGAAGAGGAGGCCCATCAGACCTCCCAGGCCGAGGGTGATTTCTTGAG TACACCCCGCCACTGGCGTATGCATTGGGAGGACGCGGTGCGTCTCAGCGCTCAGGAGAGCGTACAGCAG GCAGGCGCAAAAGTCATCCCCGGGGCTGCGGAGGGCTCATCCGACCTAGCCCAGCTTCTGGCTGAGCTCG GCGGTGTGGTTCGCCACGACCTGCAGAAGGTGCGCTTAGAGATGCAGCCCGCTTACGAAGCCACCGACTT CCCAGTGTGGGAGACCTACTTGCGTGCCTTCCACAGCGCTGTGGCCCAGCGTCTACAGGAGCTGGCGCGA GACGCCCGCGGCTGTGAGCAGCTCTATGTGCTGTTAGACTGGGCCGCTAATGTCTATGGCAGTCCTGACT TCCTGGGCGCCCCAGACTTGGCGCTGCCCACCGAGCCGCTGCCCCCACTTCTAGAGCCTGCTCTGTGGGC TCGACTGGAAAGTGACTACACTAGCTTCCTAGAGACCAAGATCACAAGCTGTTTTGACAGCATCTTGCAG CTGGAACAGAATCGCTGGGAAGCCGACGAGGACCGGGAAGTACTGCAGGGCCTCTACCACGCGCCCCTGT CCATCGATGTGCACATGCTGGTAGCTGAGCATGTGAAGGCAGCTGGCGCTATCTCCGCGGAGCTGGAGGC CACTACCCTGCAGATCTGCGCGCGTGCGCTCTGTCTCTTTGTGCCCAGGTTTGAAAAGGCTTTTCTGGCG TCGAAGGCAGTGAGCGAATGGTACCTGGGTGCTTACATTAACGCCTGCGTGGAGCTGAGAACCAGTCTTC TGGCCAGGTTCCCAGGAACCATAAAGGAACTGGAGAAACCCCTAGTGGCTGCCACCAATAGCTTCCAGAA ACATTTGCTCCAGATTGTACAGCAAGACATGCAGCCTCTATTTAAGGTGCTATATACCAAGAGCTGGCTC ACACAGGACACACTTCGTCCCCTCATGGACAAAGTGGTGGACTTTGCACATCATCTTGAGCATGTGACCC CACCACTGGCACAGGAGACACTGCAGGAGGTGCACCGTTTTGTGGTCCGCGAATACCTGGGGCAGGTGCT GAGGCCCCACGAGAGGTTCAGTGGCCAGGATCGACTGAAGGGCTCTAATAAGATGAACCTGGATGCCCAG GCCATTAGCAACACCTTCCAGGGCTTGGGCTCTGAAGCCAAGTGGCTGGACCAAGCCATCCTGTCTGTGG CTGAGATCCTGGGCGAGACATACAAAGACGACATCCGGCGGCACCTGGAAACACTTATACGGAGCTACCC GGACATCAGGCGAGACCACATTCTGGCCATTCTGGCACTACGCAGACTCGGCCGCCGCCGGAACCACAAT CTCTTGCAACACACCCAAGACTTGCTGAGGGCTGCCCACGAGACCAGGCTCCCCTCACACCACGTGCTCT TCGAGGAGATCGAAGTGCCCACCTCTGTGGACGTGCTAATCACCTGCATCTAGGGCTGAAGGGCGAGCAG GAGCCTCGTTGGCCGAGGATCTGTCACCCTCTCCATGACTGACCCCAAGTTGGGAGCCCAGTGAGTCACT CTGGCCCTACCCCCAGCACTGAAGCCTGGGGACATGGAATTTTTGGTCTCAGCTGCCTGGCTGCTCAGCC AGAGGAGCCAGGAGATGAGGCATTCCGGGCATTTGTGGGGGAGTCTTTTGGGGCTGCGGAGTCTCCTTTC AGGACTTCAGGCCAGGGAAGGCTTCCCTTTCTAACTGCTCAGCACATCCACACTGTAGTGCGAAGCTGAG GATTCTAAAAATAAATGTGAATTAAAATAGTGTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30604610 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30604610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17655 same embryo
	EMAGE:17651 same embryo
	EMAGE:17654 same embryo
	EMAGE:17652 same embryo
	EurExpress:euxassay_006004 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source