EMAGE mouse gene expression spatial database

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EMAGE:17663

Tinagl1 tubulointerstitial nephritis antigen-like 1 ( MGI:2137617)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006003. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006003_01	euxassay_006003_02	euxassay_006003_03	euxassay_006003_04

euxassay_006003_05	euxassay_006003_06	euxassay_006003_07	euxassay_006003_08	euxassay_006003_09

euxassay_006003_10	euxassay_006003_11	euxassay_006003_12	euxassay_006003_13	euxassay_006003_14

euxassay_006003_15	euxassay_006003_16	euxassay_006003_17	euxassay_006003_18	euxassay_006003_19

euxassay_006003_20	euxassay_006003_21	euxassay_006003_22	euxassay_006003_23	euxassay_006003_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17663_wholemount_strong.wlz

17663_wholemount_moderate.wlz

17663_wholemount_weak.wlz

17663_wholemount_possible.wlz

17663_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17663_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 08 09 10 17 18 19

submandibular gland primordium

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 06 07 08 09 10

lateral ventricle choroid plexus

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 06 07 08 09 10 11 13 16

4th ventricle lateral recess

strong

regional

strong expression: see section 17 18 19 20 21 moderate expression: see section 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T111
Entity Detected:	Tinagl1, tubulointerstitial nephritis antigen-like 1 ( MGI:2137617)
Sequence:	sense strand is shown >T111 GGATTCCACCCCCCTTCCCTCCCGTCCAAGGGTGCATGCATGGGGGCCGGATCTACCCAGTCTTCGGAAC CTACTGGGACAACTGCAATCGATGCACCTGCCATGAGGGAGGGCATTGGGAGTGCGACCAGGAGCCGTGT CTAGTGGACCCAGACATGATTAAAGCCATCAACCGAGGCAACTACGGGTGGCAGGCTGGGAACCACAGTG CCTTCTGGGGCATGACCCTGGATGAGGGCATTCGCTACCGCCTGGGCACAATCCGCCCATCCTCCACTGT CATGAATATGAATGAGATTTATACGGTGCTGGGCCAAGGGGAAGTGCTACCCACTGCCTTTGAAGCTTCA GAGAAGTGGCCCAACCTGATCCACGAGCCATTGGACCAGGGCAATTGTGCAGGTTCCTGGGCTTTCTCCA CAGCAGCTGTCGCATCTGATCGCGTCTCTATCCATTCTTTGGGACACATGACACCCATCCTATCACCGCA AAACCTGCTGTCCTGTGATACCCACCACCAGCAGGGCTGCCGAGGTGGGCG
Notes:	The probe template was PCR amplified from IMAGE:600866 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:600866 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17664 same embryo
	EMAGE:17662 same embryo
	EMAGE:17661 same embryo
	EurExpress:euxassay_006003 same experiment
	MGI:4828726 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source